We narrowed to 397 results for: nam
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TypeCollection...Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825...
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Hot Plasmids: Spring 2025
TypeBlog Post...antibodies for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV viral...combined CRISPR / retrotransposon system with the pithy name "site-specific target-primed insertion through targeted...025-08877-4. Two powerhouse tools combine for dynamic protein labeling By Alyssa Shepard To fully understand... the genomic targeting power of dCas9 with the dynamic labeling power of miniTurboID, a proximity ligation... -
Rinehart Lab Phosphoprotein Reagents
TypeCollection...system ( 68292 ), they were able to demonstrate unambiguous site-specific incorporation of phosphoserine ... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...start by asking two questions: 1) Is the sample dynamic or static? 2) Is the sample thin (< 15 μm) or thick...day fluorescence microscopes (see Table 1). Thin dynamic samples Ex: monolayers of live cells with fluorescently...as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment, a Total ...in resolution, down to 10s of nanometers. Thick dynamic samples Ex: 3D cell cultures, small model embryos... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...control experiments are needed to establish the dynamic range of your assay. These experiments include ...values you can expect, thereby establishing the dynamic range of your FRET assay (Figure 2). These labeling...PMCID: PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements using improved...Titov, and Jin Zhang. "Analyzing protein kinase dynamics in living cells with FRET reporters." Methods ... -
Neurodegeneration Research Collection
TypeCollection...Methods. 2022 Mar 28. Follow APP processing and dynamics using APP and Bace1 plasmids . Capitini et al.... -
Your Top Requested Plasmid in 2016!
TypeBlog Post...PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features. ...CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though a little confusing to the uninitiated, contains... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...gRNA, facilitating the live study of chromatin dynamics at a particular locus. A limitation of this system...used to examine both inter- and intrachromosomal dynamics, and how they change in response to the cell cycle...the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human cells by... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post...backbone contains a kanamycin resistance cassette, you would plate the cells on kanamycin-containing agar..... Only cells carrying plasmids that contain a kanamycin resistance cassette will grow. However, often ... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...Cellosaurus is searchable by cell line name (recommended names and synonyms will all get you to the right...an 'h' or a 'b' written on the flask (and if the name was the correct one!) Fig. 1: HeLa cells... -
TALENs for Endogenous Zebrafish Genes
TypeCollection...nucleases to the zebrafish community. Target Gene Name TALENs Full Target Site (5' to 3'; half-sites in... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...microtubule-associated protein to regulate cell growth dynamics Article contributed by Klaus Wanisch Listen to...Torsten Wittmann’s lab at UCSF to modulate cellular dynamics with light by modifying EB1. EB1 normally sits...novel ways in manipulating and studying cellular dynamics. The photoinducible control of protein function... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...this single mRNA; you get a view of translational dynamics at the single molecule level. The coding sequence...methods increase our understanding of translation dynamics, but they come with a few caveats. In both cases...this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation of Single mRNA Molecules In Vivo... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...recent blog post. Fatima Enam: Biosensors for human milk oligosaccharides Fatima Enam is currently a graduate...Commemorative Travel Awardees: Xin Zhou and Fatima Enam. There were many excellent applicants but Xin and... -
5 Great Apps for Lab Life
TypeBlog Post...to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, or Z. Some...user can search by sequence, category of enzyme, or name to discover all the information about that restriction... -
15 Hot Plasmids from 2017
TypeBlog Post...precise genome modification. Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169 pJL-SaBE3... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...regions (excluding start and stop codons) with kanamycin cassettes and were carefully designed to allow... a Kruppel-like zinc finger protein (previously named ZBTB48) in regulating the upper-bound of telomere... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...provide timely feedback, leading to unproductive dynamics. I experienced this firsthand when it took me ...and online courses focused on leadership, team dynamics, and project management. Mentorship is another...or DISC assessments can help you navigate team dynamics effectively and foster a cohesive, productive ... -
Optogenetics AAV Preps
TypeCollection...AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI ... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post...official” name for the container to be used in cross-team communication A list of “AKA” names, or jargon...other relevant information Prescribing an official name to all of our plates, tubes, and racks meant we ... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...Fluorescent protein based FRET pairs with improved dynamic range for fluorescence lifetime measurements." ... monitor neuronal activity (voltage sensors), to name a few. Find Many FRET-Based Biosensors on Addgene's...FRET for understanding biomolecular structure and dynamics in live cells." Current opinion in structural ...