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Showing: 81 - 100 of 397 results
  1. Control AAV Preps

    Type
    Collection
    ...Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825...
  2. Hot Plasmids: Spring 2025

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    Blog Post
    ...antibodies for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV viral...combined CRISPR / retrotransposon system with the pithy name "site-specific target-primed insertion through targeted...025-08877-4.  Two powerhouse tools combine for dynamic protein labeling By Alyssa Shepard To fully understand... the genomic targeting power of dCas9 with the dynamic labeling power of miniTurboID, a proximity ligation...
  3. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...start by asking two questions: 1) Is the sample dynamic or static? 2) Is the sample thin (< 15 μm) or thick...day fluorescence microscopes (see Table 1). Thin dynamic samples Ex: monolayers of live cells with fluorescently...as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment, a Total ...in resolution, down to 10s of nanometers. Thick dynamic samples Ex: 3D cell cultures, small model embryos...
  4. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...control experiments are needed to establish the dynamic range of your assay. These experiments include ...values you can expect, thereby establishing the dynamic range of your FRET assay (Figure 2). These labeling...PMCID: PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements using improved...Titov, and Jin Zhang. "Analyzing protein kinase dynamics in living cells with FRET reporters." Methods ...
  5. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features. ...CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though a little confusing to the uninitiated, contains...
  6. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...gRNA, facilitating the live study of chromatin dynamics at a particular locus. A limitation of this system...used to examine both inter- and intrachromosomal dynamics, and how they change in response to the cell cycle...the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human cells by...
  7. Cellosaurus: A Cell Line Information Database

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    Blog Post
    ...Cellosaurus is searchable by cell line name (recommended names and synonyms will all get you to the right...an 'h' or a 'b' written on the flask (and if the name was the correct one!)    Fig. 1: HeLa cells...
  8. Visualizing Translation at the Single Molecule Level

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    Blog Post
    ...this single mRNA; you get a view of translational dynamics at the single molecule level. The coding sequence...methods increase our understanding of translation dynamics, but they come with a few caveats. In both cases...this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation of Single mRNA Molecules In Vivo...
  9. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, or Z. Some...user can search by sequence, category of enzyme, or name to discover all the information about that restriction...
  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...precise genome modification. Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169  pJL-SaBE3... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...regions (excluding start and stop codons) with kanamycin cassettes and were carefully designed to allow... a Kruppel-like zinc finger protein (previously named ZBTB48) in regulating the upper-bound of telomere...
  11. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...provide timely feedback, leading to unproductive dynamics. I experienced this firsthand when it took me ...and online courses focused on leadership, team dynamics, and project management. Mentorship is another...or DISC assessments can help you navigate team dynamics effectively and foster a cohesive, productive ...
  12. Optogenetics AAV Preps

    Type
    Collection
    ...AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI ...
  13. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...official” name for the container to be used in cross-team communication A list of “AKA” names, or jargon...other relevant information Prescribing an official name to all of our plates, tubes, and racks meant we ...
  14. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...Fluorescent protein based FRET pairs with improved dynamic range for fluorescence lifetime measurements." ... monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based Biosensors on Addgene's...FRET for understanding biomolecular structure and dynamics in live cells." Current opinion in structural ...
Showing: 81 - 100 of 397 results