We narrowed to 883 results for: prot

...abundance of the protein in the sample. More protein causes a thicker band, while less protein leads to a thinner...the presence or absence of a protein, but can also determine if proteins are being up- or downregulated...modifications, quantify protein levels relative to standards, detect the cellular location of proteins, and can be... western, the protein’s secondary and tertiary structures remain intact and the protein is separated through... For better protein separation, vary the acrylamide content of the gel. For small proteins, use a higher...mix of proteins with a broad size range to be separated on a single gel.  Immobilizing proteins on a membrane...albumin or other proteins. The sticky areas on the membrane will bind to the proteins in the buffer, blocking...

...tag elements (protease and degron) and the protein of interest, there is also a protease recognition site...specific protease inhibitor, asunaprevir, the viral protease can no longer self-cleave and the protease and...tagged protein. In this event, the degron domain shuttles the entire tagged protein to the proteasome for...polyubiquitinate the fusion protein. This will target the protein of interest to the proteasome for degradation. ...AID-tagged protein which will target it for proteasomal degradation. Since its first use as a protein regulation...expressed proteins. Here we will review what advantages degron tags can offer over other protein control...are a class of protein tag which can be added to either the N or C terminal of a protein and can inducibly...

... a single protein that can recognize the target protein (Wang et al., 2013). These proteins are also plasmid-derived... the presence of proteins from samples containing a mixture of proteins. The proteins are separated based...to study protein-protein interactions. Flow cytometry Flow cytometry is used to detect proteins on the ...scientists to target specific proteins they’re interested in. Once a protein is targeted with an antibody...can visualize the protein via fluorescence or chemiluminescence, precipitate the protein out of solution...entirety of the target protein: instead, it recognizes a small portion of the protein, known as an epitope... the target protein because the antibodies will recognize multiple epitopes on the protein. However, each...

...single protein? Oftentimes, they rely on protein purification. In this strategy a single protein can be...tag, on the protein. While a variety of methods can be used to successfully isolate proteins, this blog...a recombinant protein of interest. The tag was developed as a means to purify a protein based on the affinity...and a sample containing the protein of interest flowed through. The protein of interest binds to the metal...of wash steps removes excess proteins and other contaminants. The protein of interest is then eluted using...polyhistidine tagged protein of interest (purple) and several contaminating proteins (blue, green, orange...interaction of the contaminating protein but not that of the protein of interest. A high imidazole elution...

...works by bringing together an anchor protein and activator protein to drive transcription. The anchor consists...off all mRNA binding proteins as it travels along the mRNA to produce the protein product. The system,...RNA Imaging by Coat protein Knock-off, is made up of three components: a GFP protein that binds to the ...of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of the reporter...and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins that are bound...the nucleus (thanks to a NLS on these proteins). The RFP proteins, however, remain bound to the mRNA since...phage PP7 coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein binding sites ...

...method for identifying protein-protein associations. This method uses a bait protein fused to a biotin ligase...PrM N and C   Detecting protein-protein interactions with DULIP Proteins rarely act alone. Molecular...which “bait” proteins are tagged with Protein A-Renilla luciferase and the “prey” proteins are tagged with...ligase to biotinylate proximate proteins in the cell. These candidate proteins can be enriched via biotin ...of fusion proteins, requires less biotin, and shows enhanced labeling of proximate proteins. Overall BioID2...BioID2 improves the efficiency of screening for protein–protein interactions. The Roux lab has deposited HA..., post translational protein fusion would be advantageous when individual protein expression is preferred...

...perfect world, protein concentration in your sample would directly correlate to protein abundance on your...relationship between protein abundance and band intensity is an S-shaped curve. Sparse proteins may not be detected...choose. Housekeeping proteins as loading controls Ubiquitously expressed proteins like β-actin or GAPDH... the same blot.   Total protein measurements as loading controls Total protein measurements are an accurate...the β-actin (B) or total protein (C) on the membrane versus the amount of protein loaded on the gel (n=3...linear than the total protein quantification. This illustrates why total protein measurement is often ...of total protein stains as loading controls: An alternative to high-abundance single-protein controls ...

...interactions in the cell, including protein-protein, protein-DNA and protein-RNA interactions. The 1980s saw...screening protein-protein interactions. In later years, improvements to yeast transformation protocols also...probe for detecting protein-protein interactions in mammalian cells based on protein splicing. Anal Chem... including protein expression and correct folding, post-translational modification, protein degradation... expression of target proteins in heterologous systems (e.g. mammalian proteins in S. cerevisiae). ...configuration of your fusion proteins physically blocks the binding sites for the protein partner or the UAS/reporter...C-terminal fusions of these proteins. This way both ‘ends’ of your protein are screened for binding. Using...

...antibodies bind to your protein of interest.  The next step is to detect the protein-antibody pair. In a ...to detect a single protein within a mixed sample. However, in this case your protein of interest is pre-incubated...to your protein of interest) will bind to the competing antigen in the wells while the protein-antigen...purified protein allows you to characterize properties of an antibody that recognizes that protein, while...low-abundance proteins. Figure 4: Overview of using Indirect ELISA to quantitatively measure protein concentration... of proteins in an ELISA well As mentioned above, the measurement of the concentration of proteins in ... experiments that require scientists to detect proteins in their samples. One technique that relies heavily...

...isolate a protein, there must be a special feature about that protein that no other protein in the expression...affinity tags can make protein purification a cinch. Types of affinity tags, the proteins they work best attached...common tags available. Maltose binding protein Maltose binding protein (MBP) is a 45 kDa tag which can be ...solubility of the tagged protein (Fox and Waugh, 2002). The tag can also be proteolytically cleaved after purification...unlikely to perturb protein function. This makes it amenable to being left on your protein of interest, especially...Wingfield, P. T. (Eds.). (2001). Current Protocols in Protein Science. John Wiley & Sons, Inc. https:/...Maltose-Binding Protein as a Solubility Enhancer. In P. E. Vaillancourt, E. coli Gene Expression Protocols (Vol....

...tag endogenous proteins for studying protein expression, localization, and protein-protein interactions....fluorescently tagged proteins can lead to improper protein localization, protein aggregation, or disruption...used to track protein localization, cell structure, intracellular trafficking, and protein turnover rates...disruption of normal protein function, and ultimately misinterpretation of the protein’s cellular role. One...interest to better understand normal protein function. However, CRISPR protein tagging does have its limitations...donor plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP...for endogenous protein tagging, please visit our website CRISPR/Cas Plasmids - Protein Tagging. Beyond...

...of a responsive protein (e.g. calcium binding protein) fused to two fluorescent proteins. When the responsive...fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins to make ...biosensors. NanoLuc®-fluorescent protein fusions for imaging Fluorescent proteins are fantastic imaging tools...penetrative orange fluorescent protein fusion (OgNLuc). Both fluorescent proteins can be excited by Nluc emission... imaging multiple proteins at the same time in live cells using fluorescent proteins (FPs) can be problematic...responsive protein binds to calcium it changes conformation and brings the two fluorescent proteins together... energy from one fluorescent protein to a compatible fluorescent protein. FRET can be measured by exciting...

... the same protocol for generating lentivirus, but you use a different envelope glycoprotein instead of...of the wild-type glycoprotein. The pseudotyped protein is encoded on the envelope plasmid which contains...pseudotype with the SARS-CoV-2 spike protein, scientists encode the spike protein on the envelope plasmid. Image...used envelope protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric... trimeric protein that binds phosphatidylserine and low-density lipoprotein receptors (LDLRs) on a cell...Other envelope proteins for pseudotyping While VSV-G is the most commonly used envelope protein for pseudotyping...retroviral envelope proteins have been pseudotyped on lentiviral particles. Rabies glycoprotein The rabies glycoprotein...

...MitoTimer proteins are derived from the Timer protein - a mutant of the fluorescent protein dsRed that...groups to monitor protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a... synthesized (green) protein and organelles with older (yellow to orange) protein. Image courtesy of Roberta...1852-61. A quicker method for protein inactivation Your protein of interest does x, but it also participates...your endogenous protein of interest and co-expressing its replacement recombinant protein with a FKBP domain...outer membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP) ... fused to proteins of interest for EM imaging, or targeted to subcellular regions or protein complexes...

... the pocket of a folded protein, or blocked by something bound to the protein, the antibody can't bind... of the proteins to the microtiter walls for ELISAs and a more accurate separation of proteins by mass...working with native proteins instead of denatured ones - well, don’t relax just yet. Proteins often change shapes...looking for proteins that are interacting with one another, or if you’re looking for a protein of interest...you want to capture the protein bound to anything? Is it a transmembrane protein, or is it know to translocate...browse the collection, you'll likely find some proteins of interest for which you have several antibodies... are there so many options for a single target protein? Epitope Availability The answer is simple: epitope...

...during protein translation (Ermolenko et al., 2007). All that is required is that the proteins/nucleic...fluorescent donor to your protein is to hardwire the molecule directly into the protein’s peptide sequence. Thus...you are inserting about 20-25 kDa of protein mass into your protein and so structural changes due to this...target protein, but even these modifications can affect the structure or function of certain proteins. Rather...your protein of interest: you just don’t know it yet! The poly-histidine tag often used for protein purification...NTA-sepharose beads used for protein purification: the NTA binds to the His tag on your protein and the coupled Cy... the same protein and a negative control experiment conducted on donor-only labeled protein that is incubated...

...Fluorescent Protein (GFP) and other fluorescent proteins revolutionized live-cell protein detection, their...clinically-relevant proteins are also the hardest to study. One common strategy is protein overexpression ...capture true protein behavior. Traditional epitope knock-ins maintain the native protein context, but ... or unique surface epitopes for many proteins, but some proteins do not have such epitopes. These classical...tracking proteomic cargo flow via EVs. Find ADAR-HiBiT Fusion Vector at Addgene! Watching protein degradation...Studying proteins in their natural context is one of the biggest challenges in biology. From tumor suppressors...only snapshots rather than continuous views of protein activity. Together, these limitations create challenges...

...combinations of protein tags (His, Avi, and GFP) as well as protease sites to facilitate protein purification...plasmids contain peptides to facilitate protein folding and/or protease sites to remove the epitope tags. Different...the system Easing the Protein Purification Process with pCri Cell-free protein expression kit Cell-free.... The Cell-Free Protein Expression Kit has been used to produced recombinant proteins for in-vitro assays...binding proteins in as little as 3 days. Abil et al., 2014 show that you can fuse custom PUF proteins to a...Cloning Kit BIOFAB Plasmid Set pCri System Cell Free Protein Expression Kit FREQ Seq PUF Assembly Kit Below...of interest into both sequencing (pInitial) and protein production (a variety of pExpression vectors) plasmids...

...favorite protein in your hand? At 3D Molecular Designs, we create physical models of proteins using 3D...images of proteins. But they are still flat. To truly appreciate the 3D structure of these proteins, it is...physical models of protein structure? We have been creating physical models of proteins for researchers ...While a physical model of a protein might represent one aspect of the protein very well, it will almost ... of all proteins in general, and the amazing conformational flexibility that the Cas9 protein must have...Molecular Designs. Have you ever held your favorite protein in the palm of our hand? Well, actually – have ...to hold one. Why do we need physical models of protein structure? Structural biologists have always struggled...

...be isolated from the serum. Protein A/G purification Protein A or Protein G purification is one common...or not, while any other proteins pass through. However, protein A and protein G each have different affinities... containing the target protein, and any antibody recognizing the target protein remains bound to the column...immobilized serum protein from other species. Antibodies that don’t cross-react with the serum proteins pass through...target protein due to their ability to recognize many different epitopes of the target protein. While ... detect a protein in low abundance or whose conformational state may change. If your protein might become...mixture of many antibodies that recognize the same protein. In the immune system, antibodies are produced ...