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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 303 results for: rigi

Showing: 81 - 100 of 303 results

  1. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...The resulting chimeric antibody would have the original variable regions from the parental rat antibody...antibody retains the binding specificity to the original target and can be used with secondary antibodies...

  2. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...prevent the invading genome from “recovering” its original sequence. If you’re looking to disrupt gene function...

  5. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...continue to produce the specific antibody that the original B cell produced. Because of this, all of the antibodies...reproducible experimental results. When you are originally selecting a hybridoma, you can choose one that...

  6. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... lab, the vectors must contain a yeast-specific origin of replication (ORI) and a means of selection in...ORI and antibiotic selection markers.  The yeast origin of replication (Please note: This first section...

  7. All in a Twist: dsRNA

    Type
    Blog Post
    ...to distinguish between RNA of cellular vs. viral origin and respond appropriately. When these sensing systems...10.1101/gr.237362.118 Chen, G. Y., Hur, S. Cellular origins of dsRNA, their recognition and consequences. Nat...

  8. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...overhangs. Entry DNA overhangs may be present in the original plasmid (Option 1) or added using PCR-based amplification...containing the Type IIS enzyme and ligase. Although the original destination vector + insert may spontaneously ...

  9. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...number of important plasmid elements – promoters, origins of replication, protein tags, and antibiotic resistance...use in genome manipulation. Cre recombinase, originally named because it “causes recombination” (although...

  11. Design Tips for Prime Editing

    Type
    Blog Post
    ... Editing, and that meant rereading many of the original papers reporting various prime editing tools. ...your curiosity, we encourage you to check out the original papers for more details. You can find many software...

  12. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...about some other common plasmid features, such as origins of replication and promoters, check out our other...exciting molecular technologies!  This post was originally written by Jessica Welch in January 2017 and ...

  13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...Survival strain. For plasmids that contain the R6Kγ origin of replication, choose the Pir1 strain. If your...with scientists around the world. This post was originally written by Tyler Ford in March 2017 and updated...

  14. CRISPR in the Clinic

    Type
    Blog Post
    ...post. From Mice to Men When this article was originally written, the main focus was on editing animal... and targeted therapy possible. This post was originally written in 2014 by Kendall Morgan and updated...

  15. Tips for Technical Support Calls

    Type
    Blog Post
    ...majority of our plasmids and pooled libraries were originally described in a peer-reviewed scientific publication... our thousands of depositors. That means their original articles are your best bet to figure out how to...

  17. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...applications. Get More Cpf1 Background Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN ... What we still don't know about Cpf1 In their original paper characterizing Cpf1, Zetsche et al. suggested...

  18. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...a fisherman would reel in a big catch. It was originally suspected that Cas3 moved a couple of kilobases...mutations if the chromosome is repaired with all the original DNA. With Cas3, you don’t have to play these odds...

  19. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ... Protein & Cell. Update (August 3rd, 2017) THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN .../ssDNA complex in the cell. As proposed in the original article, to achieve greater efficacy, researchers...

  20. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...Copy number is partially specified by a plasmid’s origin of replication, but can also be affected by the... lysate volume limits, the copy number of some origins of replication (e.g. p15A, ColE1) can be increased...
Showing: 81 - 100 of 303 results