...including plasmids for use in CRISPR and Tol2 transposase induced transgenesis.  Xenopus Xenopus, or frogs...

... institute is located. For example, salaries in Kansas versus salaries in NYC will vary widely. Therefore...

...Last Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility...

...editing with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol 38:824–844...

...research setting, but these events could have disastrous health effects in the clinic. NGS-based methods...

...funding are critical to averting a potential climate disaster. However, policy makers use these two fingers ...

...5): p. 1174-1174 e1. PubMed PMID: 22632979. 14. Sasaki, K., et al., Robust In Vitro Induction of Human...

...GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp...Tn7-like transposases found in diverse species of microbes. However, synthetic dCas9-transposase fusions...recruits the transposase proteins to the target location in the genome, where the transposase then carries...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases..., and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems like piggyBac or Tn7 allow... elements into the genome. CRISPR-associated transposases ( CASTs , developed by the Feng Zhang lab ) ...) combine the large-scale capacity of transposases with the precise DNA-targeting of CRISPR-Cas systems...

...two most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein ...differentiate between proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here...Quantification methods, such as western blots or ELISAs, are used to quantify relative amounts of protein...standard curves to quantify exact amounts of proteins. ELISAs quantify a single protein per assay and typically...verified to contain your protein of interest (in ELISAs, the standard curve can serve as the positive control...

...VPR expression plasmid that uses the piggybac transposase to integrate dCas9-VPR into the genome of target...

...glucose phosphate isomerase AMF, GNPI, NLK, PGI, PHI, SA-36 GPR17 G protein-coupled receptor 17 DKFZp686M18273...