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Showing: 81 - 100 of 321 results

  1. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...recessive gene. Ma et al. claim that CRISPR would still be helpful in increasing viable embryos, especially... in terms of editing success rate. Would CRISPR still be worthwhile for a given disease if it increased...

  2. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...chemically modified to resist degradation while still effectively serving their function of guiding Cas9...molecule) you will not have that option, but you can still choose what dye to use  What to do once you’ve chosen...

  4. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...how close is close enough for the desired edit to still occur if the cut site is sub-optimal? As with everything...Nonetheless, in an AT-rich region, these Cas9s may still leave you choosing between efficiency and location...

  5. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...make sure yours counts. Dress professionally but still like yourself. It’s better to be overdressed rather...challenges, your interviewers might.  You’ll probably still be required to give a job talk for a remote interview...

  8. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...fluorescence all have room for improvement. These sensors still have many useful applications, though, and if you...this two-part fluorescent system, and rhodopsin still functioning as the voltage sensor. FP-rhodopsin ...

  9. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...(Figure 4). When recombination occurs, the gene still functions normally because one of its exons has ...are located in intronic regions, this gene will still be expressed. You'd first screen for this specific...

  10. Transferable Skills: Negotiation

    Type
    Blog Post
    ... related to the actual data collection task but still be beneficial to both.  Coming up with creative ...with the company so they know I’m excited while still being able to take my vacation? I want to learn ...

  12. Of Myc and Men

    Type
    Blog Post
    ...and monoclonal antibodies. However, it was (and still is) infeasible to generate highly specific antibodies...EQKLISEEDL) - larger than the substance P peptide, but still quite small. However, unlike the substance P tag...

  13. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...its first steps! However, retroviral vectors can still direct the translation of proteins because they ...and transduction by vectors (which we sometimes still call “infection” as a shorthand, although it is ...

  15. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...and well-documented history of racial bias, which still impacts and influences current research. Understanding...five-team response to address this issue, but it still points to a far larger and more complex problem ...

  16. Tips for arabidopsis transformation

    Type
    Blog Post
    ...too old to transform. This transformation could still work, but the efficiency will be low. Tips: Use...plants: If you transform early, your plants will still have plenty of time to produce seeds after you expose...

  17. DNA Purification Without a Kit

    Type
    Blog Post
    ...regeneration, trace amounts of DNA (3 femtograms/ul) could still be eluted from the column, as detected by qPCR, ...break-up with your DNA purification kit, you can still reduce the number of spin columns you need to complete...

  18. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions...instance, the DBD of the fusion protein shown is still able to bind to the upstream activation sequence...

  20. The Challenges of Cell Culture

    Type
    Blog Post
    ...technique is still used today to test for HeLa contamination. HeLa contamination is still a problem today...
Showing: 81 - 100 of 321 results