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This post explains how to strip and reprobe a western blot, which is useful for detecting multiple targets or for correcting errors.

When you have multiple antibodies to choose from, does isotype matter? Yes, and understanding how can help you pick the right antibodies for your experiment.

Find tips and best practices for producing lentivirus in your lab: from choosing a packaging system to optimizing transfection and improving titers.

Get tips on writing your next successful grant.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Five steps to executing a great panel discussion - from planning to moderator control to audience participation.

Heading to SfN23? So are we, and we're excited to see you there!

There are many ways to communicate your science - so here we're covering how to pick the best one for you.

Learn how to identify negotiation skills you already use in your lab and life and develop them further as a professional skill set.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Having trouble with your transductions? Find troubleshooting tips from Addgene scientist Leila Haery.

Colony PCR is a useful technique for quickly screening for successful constructs after a plasmid cloning experiment.

Tips to help you save time when using the the Basic Local Alignment Search Tool (BLAST) provided by NCBI to compare sequence results.

Our depositors don't just deposit great plasmids - they also do some incredible science! Check out some incredible depositor research stories from 2022.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Find tips on how to write your first review article - a useful experience for any scientist.

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Protocols for plasmid cloning by PCR.