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We narrowed to 322 results for: virus

Showing: 81 - 100 of 322 results

  1. AAV for Neuronal Tracing

    Type
    Collection
    ...for rabies virus-based monosynaptic tracing. These AAV can be used with modified rabies virus to perform...that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect any neighboring...envelope protein of (EnvA) of leukosis virus. EnvA can direct virus infection specifically into cells that...neuronal tracing, to be used with modified rabies virus. Viral...tracing. Note : Addgene does not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal Tracing ID...Can be used to complement deletion-mutant rabies virus AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...can be used to complement deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G...

  2. Fluorescence Titering Assay

    Type
    Protocol
    ...cells with fluorescence V V = Virus Volume, mL For example: If 15 µL of virus added to 150,000 cells resulted...Fluorescence Titering Assay for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols...the cells overnight. If using freshly collected virus, filter through a 0.45 μm polyethersulfone filter...freeze-thaw. If you are freezing and aliquoting virus, it is recommended that you titer from the frozen...titer associated with freeze-thaw. If using frozen virus, rapidly thaw the lentiviral aliquot on ice prior...the dilution factors (method 1) or the volume of virus (method 2): Method 1 Method 2 $$T = {N*F*D\over ...used in downstream applications. Safety Warnings Lentivirus is generally considered biosafety level 2+. Please...

  3. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...ultracentrifugation to get pure adeno-associated virus (AAV) preps for use in your research....Ultracentrifugation You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction This protocol...an isomolar density gradient medium suitable for virus purification and isolation of cells, organelles,... remove iodixanol prior to use of your purified virus. Nonetheless, we recommend performing a buffer exchange...fewer fractions once you have a good idea when the virus elutes from the gradients. In a biosafety cabinet..., or you will lose a significant amount of your virus. When the first fraction is collected, move the ... off the walls of the filter to recover as much virus as possible. Store at 4 °C for short term (2 weeks...

  4. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...labelled with an AAV1 Cre virus combined with a Cre-dependent AAV9 tdTomato virus in the mPFC.  The AAV9 ...

  5. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...they vastly outnumber the virus particles. Incubate the cells with the virus for 48–72 h. Gently aspirate...Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This protocol can be...downstream applications. If you’ve titered your virus beforehand, you can narrow this range according ...6-well plate will hold 5 dilutions plus one 'no virus' control well). Perform a "reverse transduction"...added to the wells that already contain 0.5 mL of virus solutions at various dilution. Make sure to use ...Transducing too many cells relative to the number of virus particles reduces the transduction efficiency, resulting...Typically, cells transduced with lower dilutions of the virus will have higher levels of expression. Consider ...

  6. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...brain, researchers inject genetically modified viruses encoding microbial rhodopsins into specific cerebral...and Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become increasingly popular for gene...cells? This can be achieved using Cre-dependent viruses, such as the optogenetic FLEx vectors in combination...cell-type promoter. Once injected into the brain, the viruses infect all the cells with an inactive opsin gene...References Abdallah, Khaled, et al. "Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary...

  7. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ...working with AAV or lentivirus in your research, you might want to use one of our virus backgrounds. Addgene...barcoding libraries, and more. Download the green virus background.           Maybe your viral research...well as transfer plasmids. Download the orange lentivirus background.         We also have ready-to-use...background for you, too! And we have a COVID-19 and coronavirus collection page, where you can find plasmid tools...

  8. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...components of one virus (such as HIV, murine leukemia virus, and vesicular stomatitis virus G) are packaged...the study of the live virus. Alternatively, it is possible to generate pseudovirus, where the structural...suitable for generating pseudotyped virus for SARS-CoV-2 and other coronaviruses using appropriate packaging ... Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses (184 KB) Addgene Blog Posts Viral ...-19 - Viral Pseudotyping SARS-CoV-2 Pseudotyped Virus Mechanisms of SARS-CoV-2 infection remain poorly...packaged as replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope...envelope surface. Pseudotyped virus particles can then be used to infect permissive cells that express ACE2...

  9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...introduced into bacterial or eukaryotic cells via a virus or viral vector. One example are bacteriophages ...can be done with lentiviral and Adeno Associated Viruses (AAV). Lentiviral and AAV can be used to create... basic molecular biology, plasmid cloning, and viruses. References Baltrus, David A., and Karen Guillemin...bacteria have gained antibiotic resistance and virulence. Scientists have studied how HGT occurs in nature...

  10. Clear and Accessible Writing

    Type
    Blog Post
    ...ultracentrifugation so that a scientist can extract virus from the correct layer.  Third, think about the ...at the interface of the 40% layer containing the virus and 60% iodixanol layer, with the bevel pointed ... the tube allows air to enter the column so the virus will flow out the bottom syringe."Feel free to share...

  11. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...which types of cells a virus can infect. The VSV-G envelope protein enables this virus to infect many different...lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled library screening...posts Read our viral vector blog posts Read our lentivirus guide Resources on the Addgene website Browse...

  12. Viral Vector Guides and Plasmids

    Type
    Collection
    ...-type virus propagation and use of viral plasmids to produce viral vectors. A) Wild-type viruses infect...BioRender.com. Wild-type viruses have one goal, which is to make more viruses. The viral genome contains...the host’s machinery to produce more viruses. In this way, viruses are excellent vehicles to deliver genetic...propagate in the same way that wild-type viruses do. For general virus biosafety guidelines, check out Addgene's...transduce dividing and non-dividing cells. These virusal vectors enable long-term stable gene expression...Vector Type Expression Genome Packaging Capacity Virus Size Cells Infected Host Genome Integration Immune...the necessary genes to achieve this goal. When a virus recognizes a host cell, it injects its viral genome...

  13. Adenovirus Plasmids

    Type
    Collection
    ...Plasmids Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors...101 eBook Adenoviruses are a class of medium-sized, non-enveloped, double-stranded DNA viruses known to...levels of transgene expression. Additionally, these viruses are able to target a broad range of dividing and... Bunz 16402 pShuttle Shuttle For production of viruses containing transgene under a chosen promoter Vogelstein...Vogelstein 16403 pShuttle-CMV Shuttle For production of viruses containing transgene under CMV promoter Vogelstein...pAdTrack Shuttle For production of GFP-trackable viruses containing transgene under a chosen promoter Vogelstein...pAdTrack-CMV Shuttle For production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein...

  14. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...that offer his constructs. Importantly, control viruses (not expressing the opsin) are also available. ... optogenetics experiment you may need to inject virus into 20 or more mice and the injections must be ... practice with die injection prior to using the virus. You may need to adjust coordinates based on your...co-localization immunofluorescence, cell-type specificity. Viruses that only express the fluorescent proteins are ...affects the length of time you may need to allow the virus to express. For example, if you inject into the ...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral therapies such that the virus is undetectable... undetectable in the blood. But the virus doesn’t completely disappear; it just hides in latently infected...infection, which would make it easier for an “escape” virus to propagate and infect adjacent cells. It will ...of viral DNA efficiently suppresses hepatitis B virus. Sci Rep. 2015 Jun 2;5:10833. PubMed PMID: 26035283...

  16. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...delivery vehicle that’s based on mouse leukemia virus (MLV) virus-like particles (VLPs). VLPs lack viral genomes...box.  Nanoblades are engineered murine leukemia virus (MLV)-like particles loaded with Cas9-gRNA ribonucleoproteins...with a mix of VSV-G and the baboon endogenous retrovirus Rless glycoprotein (BaEVRless) had high rates...

  17. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...of the upstream efficiency element of the simian virus 40 late polyadenylation signal by using in vitro...Donello JE, Trono D, Hope TJ. Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances ...H-G. Multiple copies of the Mason–Pfizer monkey virus constitutive RNA transport element lead to enhanced... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to...

  18. Lentivirus Production

    Type
    Protocol
    ...replace with fresh media. Day 3-4 (am): Harvest virus Equipment Class II, Type A2 Biological Safety Cabinet...DMEM Complete or OptiPro SFM. Incubate the cells. Virus can be harvested at 48, 72, and 96 h post transfection... Protocols Lentivirus Production Lentivirus Production You may also ...this protocol to generate lentivirus Protocols...Introduction This protocol can be used to produce lentivirus from a lentiviral vector transfected into 293T...lines or transfection reagents. Once produced, lentivirus can be used for a variety of downstream applications...

  19. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Adeno-associated viruses (AAV) are small viruses originally discovered as contaminants of adenovirus stocks. One... presence of another "helper" virus, such as adenovirus or herpes virus, in order to propagate. This is...Viral Plasmids Adeno-associated Virus (AAV) Plasmids Adeno-associated Virus (AAV) Plasmids Additional Resources... requirement has been circumvented with “helper-virus free systems,” which enable the production of infectious...infectious AAV particles without the use of a helper virus. Instead, specific gene products can be provided...infectious AAV particles, a helper plasmid (encoding adenovirus E4, E2A and VA) and a RepCap plasmid (encoding... Helper plasmid for AAV packaging, expressing adenovirus E4, E2A and VA James M. Wilson RepCap Plasmids...

  20. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...how a phage recognizes its host Bacteriophages (viruses that prey on bacteria) may be the most numerous...Simpson, Sacher, and Szymanski in January 2016 in Viruses (5). Please refer to the paper for more details...Function of Modified Bases in Bacteria and Their Viruses." Chemical Reviews (2016). PubMed PMID: 27319741...Receptor Binding Proteins from Bacteriophages." Viruses 8.1 (2016): 17. PubMed PMID: 26761028. PubMed Central...
Showing: 81 - 100 of 322 results