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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,019 results for: CAL;

Showing: 1001 - 1019 of 1019 results

  1. Kit Free RNA Extraction

    Type
    Protocol
    ...the pellet. Air-dry the pellet for 5-10 minutes. Critical It is important to not let the pellet get too ...

  7. Modular Cloning Guide

    Type
    Guide
    ...Johannes Stuttmann 95 additional plasmids for hierarchical assembly of plant multigene constructs. MoClo...characterized parts that can be used for bottom-up hierarchical assembly of single and multi-gene constructs...Assembly Kit Bacterial Expression Christopher A. O'Callaghan Toolkit for assembling large DNA fragments based...and Multisite Gateway cloning. This system was specifically designed for generating zebrafish transgenesis...

  8. Addgene's eBook Collection

    Type
    Guide
    ...2021 | Third Edition About This eBook covers practical experimental advice such as tips for planning ... on FRET, biosensors, and monitoring protein localization; an introduction to non-protein fluorophores...First Edition About Our newest eBook contains practical introductions and helpful tips for antibody-based...

  9. Guide to Using Pooled Libraries

    Type
    Guide
    ...Genome-Scale, Knockout Screening Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional...plasmid, think of each cell carrying it as being a biological replicate for that plasmid. Library screens can...

  10. Adenovirus Guide

    Type
    Guide
    ...be included between the ITRs is limited by the physical space available inside of the adenoviral capsid...contain E4. Download the Bert Vogelstein’s lab practical guide for using the AdEasy™ system . Find plasmids...packaging cell line. Recombinant adenoviral vectors typically do not integrate in the host genome and instead...

  11. Sequencing Primers

    Type
    Guide
    ...IRES, reverse primer L4440 AGCGAGTCAGTGAGCGAG (Caldwell lab) 5' of MCS in L4440 vector, forward primer...LacZ, reverse primer LexA CGTCAGCAGAGCTTCACCATTG (Caldwell lab) 3' end of LexA DNA binding domain, forward...

  12. Cloning

    Type
    Guide
    ...is done via a single recombination reaction, drastically simplifying the process and reducing the amount...TOPO Cloning ) Toposiomerase based cloning, often called TOPO® cloning or TA cloning, is a method that relies...

  13. DNA Quantification

    Type
    Protocol
    ...Calculate your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol....

  14. Protocol - How to Design Primers

    Type
    Protocol
    ... template region of DNA. They are synthesized chemically by joining nucleotides together. One must selectively...

  19. Science Guides

    Type
    Guide
    ...Chemogenetics Chemogenetics is a technique that uses genetically engineered receptors to selectively control and...
Showing: 1001 - 1019 of 1019 results