We narrowed to 1,023 results for: NTS

1. Viral Vectors 101: Chemogenetics

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   Blog Post

   ...your results are viable for translation to human patients. Picking receptors The receptor you choose is ...

2. Transferable Skills Guide: Managing a Team

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   Blog Post

   ...found this to be particularly challenging. As PhD students and postdocs we are used to doing (almost) everything...

3. Transferable Skills: Negotiation

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   ...hoping that your lab mate would have time to make comments on your manuscript. If you give her this slot,...

4. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

   ...cytometry has the ability to generate incredible amounts of data from a single experiment. Although there...

5. Pooled Library Amplifications

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   Blog Post

   ... Genome-wide Inhibition Library. DNA and cell amounts After deciding on a cell type, you will need to...

6. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...

7. Plasmids 101: Secondary Nanobody Toolbox

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   ...Nanobodies also simplify multicolor staining experiments because they don’t require using primary antibodies...

8. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   ... the challenges you face in your career.  Acknowledgements: I would like to thank fellow Addgenies Joanne...

9. Plasmids 101: Colony PCR

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   ...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

10. Plasmids 101: Protein tags

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    Blog Post

    .... You can get your bacteria to produce massive amounts of protein, but if it’s not folded correctly, there...

11. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ... fluorescent protein to use in your upcoming experiments. In the meantime, if you haven't checked out ...

12. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...due to both on- and off-target effects, but also presents a strategy to control for these effects. By testing...

13. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post

    ...appreciate the opportunity to share these invaluable reagents with the scientific community. Note: Cary Valley...

14. Viral Vectors 101: Pseudotyping

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    Blog Post

    ...King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...

15. Hot Plasmids: Fall 2024

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    Blog Post

    ...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...

16. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows for the ...

17. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post

    ...Although the DE3/T7 RNAP system works well for most experiments, the lac promoter can “leak,” meaning that a ...

18. Five Popular Model Organisms

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    Blog Post

    ...hermaphrodites making it easy to conduct large experiments over multiple generations. Similarly to other...

19. Quick Guide to Near-Infrared Fluorescent Proteins

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    Blog Post

    ...biosensors NIR reporters can tell us about cellular events via  fluorescence changes occurring due to protein...

20. Antibodies 101: Beyond Surface Labeling

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    Blog Post

    ...surface of the CD8+ T cells and gate on the positive events for your flow analysis. However, certain downstream...