We narrowed to 1,022 results for: NTS

...found this to be particularly challenging. As PhD students and postdocs we are used to doing (almost) everything...

...hoping that your lab mate would have time to make comments on your manuscript. If you give her this slot,...

...cytometry has the ability to generate incredible amounts of data from a single experiment. Although there...

... Genome-wide Inhibition Library. DNA and cell amounts After deciding on a cell type, you will need to...

... the challenges you face in your career.  Acknowledgements: I would like to thank fellow Addgenies Joanne...

... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...

...Nanobodies also simplify multicolor staining experiments because they don’t require using primary antibodies...

...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

.... You can get your bacteria to produce massive amounts of protein, but if it’s not folded correctly, there...

... fluorescent protein to use in your upcoming experiments. In the meantime, if you haven't checked out ...

...appreciate the opportunity to share these invaluable reagents with the scientific community. Note: Cary Valley...

...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...

...could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows for the ...

...due to both on- and off-target effects, but also presents a strategy to control for these effects. By testing...

...King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...

...Although the DE3/T7 RNAP system works well for most experiments, the lac promoter can “leak,” meaning that a ...

...surface of the CD8+ T cells and gate on the positive events for your flow analysis. However, certain downstream...

...biosensors NIR reporters can tell us about cellular events via  fluorescence changes occurring due to protein...

...hermaphrodites making it easy to conduct large experiments over multiple generations. Similarly to other...

...strains, especially mutations that make major improvements such as those that increase plasmid yield and...