We narrowed to 1,081 results for: ANT

... MoClo Plant Parts Kit Plant Expression Nicola Patron 95 standardized parts specific for plant synthetic...multi-gene constructs for Xanthobacter or Roseixanthobacter species. LbPlant Toolbox Kits Bacterial Expression...synthetic biology. MoClo Plant Parts II and Infrastructure Kit Plant Expression Johannes Stuttmann 95 additional...plasmids for hierarchical assembly of plant multigene constructs. MoClo Plant Parts III: Transformation & Genome...GreenGate Cloning System Plant Expression Jan Lohmann 56 plasmids to create plant expression vectors containing...generate multi-construct transgenic plants. GreenGate 2.0 Toolkit Plant Expression Alexis Maizel 68 plasmids... multi-gene constructs for plant transformation. GoldenBraid 2.0 Kit Plant Expression, CRISPR Diego Orzaez...

...binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9...LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter...PAM sequence often found in plants, expanding the targeting range for plant genome engineering. Read more...more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase the risk of off-target cutting...like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian...Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' ...Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...information on antibiotic resistance and additional antibiotics, see our blog post on antibiotic resistance... grow in or on media containing that antibiotic. These antibiotic resistance genes not only give the scientist... multiple generations. It is important to distinguish that the antibiotic resistance gene is under the...cultures with antibiotics, see Addgene’s protocol on inoculating bacterial cultures . Antibiotic Recommended...Origins of Molecular Genetics The Genetic Code Recombinant DNA Working with Plasmids Plasmid Elements Types...Molecular Cloning Common E. coli Strains Common Antibiotics DNA Sequencing Sanger Sequencing Next-generation...Additional Resources Plasmids are one of the most important tools in molecular biology. They allow scientists...

...wild-type opsin of each and some examples of relevant variants. This list is not exhaustive — please browse...Inhibitory (Hyperpolarizing) ChR Variants Alternatively, ChR variants that inhibit neurons have been created... these variants result in the hyperpolarization of neurons. Examples of anion channel variants from other...Mac and its variants allow for inhibition of neurons using blue-green light. Mac variants have been engineered... eMac3.0 Glossary of Microbial Opsin Variants Opsin Type Variant Description Peak Response Spectra (nm...Phobos Blue-shifted iC++ variant 467 Aurora Red-shifted anion-selective ReaChR variant 517 Halorhodopsins Jaws...to the membrane. Excitatory (Depolarizing) ChR Variants The optogenetic toolbox has been expanded by scientists...

...in a higher background of non-recombinant adenoviral plasmids. Recombinant adenoviral plasmids are then...Treatment Recombinant adenoviral vectors are also being used in cancer therapy to stimulate anti-tumor immune...specialized immune cells and antibody production that develops after exposure to an antigen. AdEasier-1 cells BJ5183...vectors for recombinant adenoviral vector production that do not contain GFP. Recombinant adenoviral vector...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type adenoviruses have...have been modified by researchers to create recombinant adenoviral (rAdV) vectors in order to deliver genetic...

...Guide . Recombinant AAV Vectors Wild-type AAVs have been modified by researchers to create recombinant adeno-associated...activation, both key advantages of rAAV vectors. AAV Types Serotypes AAV serotypes are AAV variants that differ...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...in order to deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the ...Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning...Created with BioRender.com. Genome Integration Recombinant AAVs are generally considered to not integrate...vectors for in vivo applications. Other Engineered Variants Hybrid capsids Hybrid capsids are engineered capsids...

... is important to confirm that there are no additional sites present within the fragments you want to assemble...Resources Molecular cloning, or the creation of recombinant DNA, is an essential process used in scientific... popular cloning methods for the creation of recombinant DNA. Restriction Enzyme Cloning Restriction enzyme... and ligated into your desired plasmid. It is important to note that restriction enzyme target sites can... fresh PCR product into a plasmid. The major disadvantage of TOPO cloning is that very few plasmid backbones...more commonly known as Gibson assembly, takes advantage of the properties of three common molecular biology...orientation at a time, and without the need for any unwanted sequence at the junctions (such as a restriction...

...(knockout)? Generate a specific mutant allele of a gene (point mutant)? Increase or decrease expression...of a selectable marker (antibiotic resistance or fluorophore) are also relevant. Delivery Using Plasmids... specific disease or process of interest. Do you want to: Generate complete and permanent loss of gene... window from the PAM sequence, and base editor variants often have different optimal editing windows. ...testing multiple pegRNA designs is particularly important for prime editing. CRISPR Intereference : Target...Predicted On-Target and Off-Target Activity Another important factor to keep in mind when designing your gRNA...In addition to off-target activity, it is also important to consider factors that maximize cleavage of ...

...release them into the supernatant. After an initial media change, the supernatant containing the viral ...gene, conferring antibiotic resistance to infected host cells. When these antibiotics are added to the...Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning...integration site preferences. A major cellular determinant is LEDGF (also known as PSIP1 or p75 ), a lentiviral...predict. In therapeutic applications, like chimeric antigen receptor T cell (CAR-T) therapy, the location of...the genetic information encoded by that genome. Antibiotic resistance is not the only type of selectable... selection marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is...

... release it into the supernatant. After an initial media change, the supernatant containing the viral ...gene, conferring antibiotic resistance to infected host cells. When these antibiotics are added to the...Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning...vectors are produced in just a few days. Another variant, Phoenix-gp, contains only gag-pol and enables ...the genetic information encoded by that genome. Antibiotic resistance is not the only type of selectable... selection marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is...immunodeficiency disorders. Delivery of chimeric antigen receptor T cell (CAR-T) therapy is a particularly...

...ligands can have high potency, which is potentially important for dosing in animal studies, made them attractive... N ‐oxide (CNO). CNO is a metabolite of the antipsychotic clozapine and seems to be pharmacologically ...however, it has off-target effects. The atypical antipsychotic drug olanzapine has also been shown to be an...domain was engineered to respond specifically to the anti-smoking drug varenicline. This PSAM carries mutations...things to consider: Activation or Inhibition? Do you want to turn ON or turn OFF the neurons in your experiment...potentially express the chemogenetic receptor in unwanted cell types. Generating a FLEx switch to control...common property of either typical or atypical antipsychotic drugs: is M1 agonism a pre-requisite for mimicking...

... below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced...system, antibodies are proteins capable of highly specific binding to other proteins. Antibody-based applications... often use antibodies conjugated to a signaling molecule, can be used to capture, quantify, and identify...

...can help you begin to understand what genes are important to a certain phenotype, and allow you to design...Both types of screen employ a selection method relevant to the phenotype being studied. Examples of published...lentiviral CRISPR libraries include: resistance to the anti-cancer drug vemurafenib , resistance to Clostridium...library to cells. The screens diverge at a few important points, including when to apply selection, when...

... Constitutive Plant Strong promoter from Cauliflower Mosaic Virus Ubi Constitutive Plant High-expression...corresponds to the mRNA transcript produced. The antisense strand is referred to as the template strand or...itself, which helps to explain how many physically distant DNA sequences can affect transcription of a given...

... Overlay Select Antibodies 101 New! October 2024 | First Edition About Antibodies 101 October 2024...contains practical introductions and helpful tips for antibody-based applications, from flow to IHC! Close About...

...your molecular biology research. Topics include: Antibiotic Concentrations for Bacterial Selection What is...

...information on molecular biology, plasmids, and recombinant DNA, please consult Addgene's Molecular Biology...

...right for appropriate antibiotic concentrations. Antibiotic Concentrations Antibiotic Recommended Stock Concentration...to be resistant to the antibiotic. On the second plate, streak out a strain that’s not resistant to the... be resistant to the antibiotic, (+) indicates that the tested strain is supposed to be resistant to the...that: The antibiotic broke down. You forgot to add the antibiotic. You added the antibiotic at too low... any antibiotic. Negative Result 3: Only the Non-resistant Strain Grows If only the non-resistant strain...one or more antibiotic resistance genes, which confer resistance to a specific antibiotic to the bacteria...resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic resistance gene. ...

...Immunocytochemistry You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Western Blot Protocol...Triton X-100 Primary antibody Secondary antibody Deionized water Microscope slide Anti-fade mounting medium...Secondary antibodies must match the host species of the primary antibody. For example, use an anti-mouse ... with a primary antibody against a target protein and a fluorescent secondary antibody. Protocols...Protocol Recombinant Antibody Purification Protocol Introduction Immunocytochemistry is a technique that...that uses antibodies to detect antigens in cells. Here we describe the basic steps for fixing and labeling... with a primary antibody against a target protein and a fluorescent secondary antibody. This protocol ...

...the primary antibody. For example, use an anti-mouse secondary antibody for primary antibodies raised in...Blot You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Immunocytochemistry...Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Introduction Western blot is a technique used...block, incubate with primary antibody Day 2: Incubate with secondary antibody Video Watch this instructional...Chemiluminescence substrate Plastic wrap Primary antibody Secondary antibody Deionized water Before Starting Refer...information specific to your antibody, such as ideal blocking buffer and optimal antibody concentrations. Consider...Consider titrating your antibody to determine the optimal dose. Secondary antibodies must match the host ...