...burdensome task of explaining the steps to a lab assistant. However, I recognized the need to shift my mindset...

...This table is by no means exhaustive, so you may want to consult the REBASE database for more detailed...

...patient mutations using SDM can help determine if a variant is causal for a given disease. CRISPR has made ...

...biology known as bioelectricity is revealing the important role of bioelectrical signaling mediated by ion-channels...

...tough assignments, the skills that Susan  finds important for her work include: “Listening. Evaluating evidence...

...Averaging ten panels each. (For this story, it is important to know that the student presenting right before...

...talk. Some video conferencing software allows participants to “raise” their hand to ask a question or type...

...function in the human body?” … and probably most importantly… "how do we know this?” Over the past fifteen...

...muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...

...simple to use, users should keep aware of a few important details. For transposons to be specifically recognized...

...cloning strain has failed so far. What is most important is the transformation efficiency of the strain...

...newly generated MCS. MXS-chaining also has the advantage of being directional, meaning that (in the previous...

...that company culture and enjoying your job were important parts of team buidling - they relieved some of...

...experimenting with open source assembly algorithms meant that we already had a clear idea of the necessary...

... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking...

...D.A., et al., Generation, purification and transplantation of photoreceptors derived from human induced...

...right for appropriate antibiotic concentrations. Antibiotic Concentrations Antibiotic Recommended Stock Concentration...to be resistant to the antibiotic. On the second plate, streak out a strain that’s not resistant to the... be resistant to the antibiotic, (+) indicates that the tested strain is supposed to be resistant to the...that: The antibiotic broke down. You forgot to add the antibiotic. You added the antibiotic at too low... any antibiotic. Negative Result 3: Only the Non-resistant Strain Grows If only the non-resistant strain...one or more antibiotic resistance genes, which confer resistance to a specific antibiotic to the bacteria...resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic resistance gene. ...

...Immunocytochemistry You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Western Blot Protocol...Triton X-100 Primary antibody Secondary antibody Deionized water Microscope slide Anti-fade mounting medium...Secondary antibodies must match the host species of the primary antibody. For example, use an anti-mouse ... with a primary antibody against a target protein and a fluorescent secondary antibody. Protocols...Protocol Recombinant Antibody Purification Protocol Introduction Immunocytochemistry is a technique that...that uses antibodies to detect antigens in cells. Here we describe the basic steps for fixing and labeling... with a primary antibody against a target protein and a fluorescent secondary antibody. This protocol ...

...the primary antibody. For example, use an anti-mouse secondary antibody for primary antibodies raised in...Blot You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Immunocytochemistry...Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Introduction Western blot is a technique used...block, incubate with primary antibody Day 2: Incubate with secondary antibody Video Watch this instructional...Chemiluminescence substrate Plastic wrap Primary antibody Secondary antibody Deionized water Before Starting Refer...information specific to your antibody, such as ideal blocking buffer and optimal antibody concentrations. Consider...Consider titrating your antibody to determine the optimal dose. Secondary antibodies must match the host ...

...mammalian cells to produce antibodies Antibody Purification Purify recombinant antibodies using Protein A or ...Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies Generating Stable ...Video! Immunocytochemistry Use antibodies to detect antigens in cells Antibody Validation Using the Indirect...testing your virus preparations. Antibodies Protocols for common antibody applications. Intro to the Lab...lab Watch the Video! Over-Agar Antibiotic Plating Quickly add antibiotic to a pre-poured plate Watch the...Stain Determine purity and concentration of recombinant antibodies Watch the Video! Western Blot Separate ...indirect ELISA against a purified antigen to demonstrate the expected antibody dose response....