We narrowed to 1,075 results for: TOR

1. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   ... you can start by paying attention to how your mentors reach solutions and ask them to explain their logic...

2. Antibodies 101: Polyclonal Antibodies

   Type

   Blog Post

   ...Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https...

3. Plasmids 101: Cre-lox

   Type

   Blog Post

   ...independently of any other accessory proteins or co-factors, thus allowing for broad applications in a variety...

4. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

   Type

   Protocol

   ...cut the vector, you will need to use a phosphatase to prevent re-circularization of the vector if you ...having difficulty finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut ...

5. Immunocytochemistry

   Type

   Protocol

   ...water Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes...paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of waste in the...remove the coverslip. Blot the coverslip with a laboratory wipe to remove excess liquid. Add 1 drop of anti-fade...

6. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   ...applied antibodies and need to dry the membrane for storage, but plan to reprobe it later, strip it thoroughly...

7. Mouse Modeling, Part 2: Breeding and Crossing Mice

   Type

   Blog Post

   ...the appropriate primers to detect your targeting vector or gene of interest. 2. Breed your parental mice...

8. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   ...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...

9. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   ...Mol Syst Biol. 2014 Galit Lahav EYFP Gateway vectors with EYFP split at 175 Arabidopsis homolog of the...

10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    ...quickly. Just make sure to incorporate the dilution factor in your calculation from OD to protein concentration...

11. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    ...specific, individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9...

12. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    ...impacts in the way we study and treat complex or multifactorial diseases, enabling faster diagnosis and treatment...

13. Using a Light Microscope Protocol

    Type

    Protocol

    ...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of... up your work area and microscope after use and store the microscope appropriately....

14. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    ...) For CrispyCrunch, we wanted to consider both factors, as a bench scientist would looking at a genome...

15. Plasmids 101: Degron Tags

    Type

    Blog Post

    ...usual. In the presence of a specific protease inhibitor, asunaprevir, the viral protease can no longer...

16. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    ...RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could be less desirable...

17. Pouring LB Agar Plates

    Type

    Protocol

    ...your plates are ready for use or storage at 4 ℃. You should not store your plates for longer than 1 month...material to reduce condensation. The plates are then stored at 4 ℃ until use. Once your plates have solidified...

18. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ...to RNA deaminase ADAR2 to create a specific RNA editor. Cas13 enzymes are advantageous because they do...

19. Lab to Office Culture Shock

    Type

    Blog Post

    ...industry, nonprofit organization, law office, journal editor, etc.--so everyone’s experience will be a little...

20. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    ...acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria. Nat Biotechnol...