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We narrowed to 1,106 results for: Ide

Showing: 1041 - 1060 of 1106 results

  6. Antibodies 101: Isotypes

    Type
    Blog Post
    ... isotypes, such as the number and location of disulfide bonds, the number of constant domains, and the...

  12. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Watch the Video! How to Design a Primer Key considerations when designing primers Watch the Video! Sequence...protocols that you can use for a wide range of applications, with videos for select protocols in the right-hand...right-hand column. You can find even more video content on the Addgene Video Page . We hope that you'll find these...BSL-2 Watch the Video! Water Baths Proper water bath maintenance and use Watch the Video! Pipetting Learn...Link to Video Making LB Agar Plates Create plates to culture bacteria in the lab Watch the Video! Over-... the Video! Streaking Bacteria Isolate single bacterial colonies on an agar plate Watch the Video! Inoculating... the Video! Polymerase Chain Reaction (PCR) Basic PCR protocol with tips and FAQ Watch the Video! Purifying...

  13. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...These lab safety guidelines provide steps to ensure you are working in BSL-1 and BSL-2 labs safely...., and exposures to hazardous materials. Video Watch the video to learn more about safety in the lab. Equipment...container Fire blanket Fire extinguisher Guidelines BSL-1 Guidelines Before You Work Right after entering ...chemical hygiene officer. BSL-2 Guidelines Remember, the BSL-1 laboratory guidelines above are expected to be...Intro to the Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction...There are four biosafety levels. This protocol provides information for both biosafety level 1 (BSL-1)...distinguish between different types of hazards and to provide protective measures for each level, as each level...

  14. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...Update: February 7, 2022 Protocol Video Watch this instructional video to learn how to affinity purify ...3216-03 5% Sodium Azide, 500 mL, VWR 7144.8-16 PBS, 1X pH 7.4, VWR 45000-446 Benzamide, Millipore Sigma...Plasmid Collection Antibody Blog Posts Antibody Guide Transfection for Recombinant Antibodies Immunocytochemistry...reproducibility in science. Safety Warnings Sodium azide is toxic. When handling, use proper personal protective...laboratory coat, laboratory goggles, and gloves. Sodium azide containing waste should be properly disposed of ...dibasic, Millipore Sigma S7907-500G 1 M Trizma hydrochloride solution pH 9.0, Millipore Sigma T2819-1L 250... capacity. If your sample exceeds the capacity, divide the sample among multiple columns. For information...

  15. Western Blot

    Type
    Protocol
    ...General Guidelines Acrylamide percentage varies in SDS-PAGE gels. Use lower percentage acrylamide when immunoblotting...Incubate with secondary antibody Video Watch this instructional video to learn how to use western blotting...antibody, such as ideal blocking buffer and optimal antibody concentrations. Consider titrating your antibody...Plasmid Collection Antibody Blog Posts Antibody Guide Immunocytochemistry Protocol Recombinant Antibody...molecular weight proteins and higher percentage acrylamide when immunoblotting low molecular weight proteins...but will generally be between 250–1000 µL . The ideal lysis buffer will vary depending on the cellular...amount of total protein for each sample. Pro-Tip The ideal total protein loaded will vary between samples and...

  16. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...contents of any literature. Literature are provided to you as a guide and on an “AS IS” “AS AVAILABLE” basis...Addgene 2006, All Rights Reserved. This protocol is provided for your convenience. See “warranty information...that can be accessed after a free registration. Guidelines for designing siRNAs with effective gene silencing...found, search for NAR(N 17 )YNN, where N is any nucleotide, R is a purine (A,G), and Y is a pyrimidine (...targeting introns. Avoid stretches of 4 or more nucleotide repeats, especially repeated Ts because polyT...polymerase III. Note that these were the prevailing guidelines when the pLKO.1 vector was created. Additional...program. Select sequences that have at least 3 nucleotide mismatches to all unrelated genes. TIP: Addgene...

  17. Protocol - How to Design Primers

    Type
    Protocol
    ...repeatedly the reactive groups on a nucleotide when adding a nucleotide one at a time. The main property ...more than one nucleotide at that position termed a mixed site. One can also use a nucleotide-based molecular...instead of a regular nucleotide for broader pairing capabilities. Taking into consideration the information...GCGGCG-restriction site-your sequence). Protocol Video Watch the protocol video below to learn how to design primers... Gel Electrophoresis Primer Design for PCR Oligonucleotide primers are necessary when running a PCR reaction.... They are synthesized chemically by joining nucleotides together. One must selectively block and unblock... the primer usually has stretches of several nucleotides. Also, both of the 3’ ends of the hybridized ...

  18. Using a Light Microscope Protocol

    Type
    Protocol
    .... The ocular lens also provides magnification and the power should be provided on the microscope; often...sample. Place your slide (or other sample type) on the microscope stage. If using a slide, you can secure... your focus slowly so that you don't accidentally hit your slide and risk damaging the lens or your sample...Intro to the Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction...which you can control using the focus knobs on the side of the microscope. You may be wondering why there...different magnifying powers. When you look at the side of an objective, you will see several pieces of ...often this lens provides 10x magnification. To determine the final magnification of your image, multiply...

  19. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ... Last Upload: May 18, 2018 Protocol Video Watch the protocol video below to learn how to analyze a restriction...Although this is never an ideal cloning strategy, sometimes it cannot be avoided. If you do have to do so...center (ideally around 1/3 of the way from one end), and also cuts in the backbone on one side of the ...recognize, restriction digests have become the most widely used method scientists employ to selectively move...including Addgene’s Sequence Analyzer , allow you to identify which restriction sites are present in a given...insert and the 5000bp backbone. Verifying Plasmid Identity by "Plasmid Fingerprinting" Often, it will be ...restriction enzymes for this approach, it is often a good idea to choose two different enzymes that will give you...

  20. Kit Free RNA Extraction

    Type
    Protocol
    ...precipitation, and resuspension. This protocol provides two options: using Solution D (see reagent section...are also included: using Isopropanol or Lithium Chloride. This protocol was adapted from (Link opens in...2006 and (Link opens in a new window) TRIzol® User Guide from ThermoFisher Scientific . Figure 1: A diagram...for precipitation step, Option A) 7.5 M Lithium Chloride (for precipitation step, Option B) Glycogen (Optional...phase without disturbing the interphase layer, consider using a lower volume pipettor like a p200 to collect...(s). You can use either Isopropanol or Lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...aqueous layer. Incubate at -20°C for 1 hour. Lithium Chloride (Option B): LiCl selectively precipitates RNA ...
Showing: 1041 - 1060 of 1106 results