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...given therapeutic approach brings to patients, doctors, and insurance providers, and is crucial to how...

...clone the PCR products into a pBluescript cloning vector and sequence the resulting plasmid. It can take...

...performing a single base pair edit with a prime editor. What next? When generating a knock-in mutation...

...currently a research scientist in the Department of Laboratory Medicine at the University of Washington. She...

...to drive cell type-specific delivery of a viral vector (Strebinger et al., 2023). All this flexibility...

...loop Bunsen burner (or other small flame source) Incubator Marker Reagents LB agar plate (with appropriate...resistance and your initials. Labeling within a laboratory setting is important for organization, and it...

...spreading of antibiotic makes it easy for an investigator to conveniently plate and select transformed...spreading Bunsen burner (or other small flame source) Incubator Reagents 6 cm diameter LB/agar plates without ...

... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to .... Water baths can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room...

...19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais ...

...a blank chart to your spreadsheet. In the Chart Editor , select the Chart Type drop down menu and select...calculate this for you as follows: In the Chart Editor , select the Customize tab. Select the Series drop...

...microcentrifuge tube. Use the lysate immediately or store at -80 °C until ready to use. Section 2: Determine...the Top Stack to one side and discard the white separator. Keep the Bottom Stack in the plastic tray. Place...

... to protein (260/280) is generally used as an indicator of the purity of DNA samples. These days, many...

...important for your experiments. There are a number of factors to consider when selecting sequencing primers, ...

... reagents for a stock mixture is an essential laboratory technique, as imprecise measurements can affect...

...especially useful for introducing promoters, terminators, and other short sequences into the assembly ...

... After all of the sample is unloaded, push the pipettor to the second stop and carefully raise the pipette...

...curve or dilute the unknown sample (don’t forget to factor in that dilution when calculating the original ...

...Viral Vectors Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...adenoviral (rAdV) vectors in order to deliver genetic cargo into cells. Most rAdV vectors are based on adenovirus...Generations First-generation rAdV vectors The first generation of rAdV vectors was developed by removing the...-generation rAdV vectors. Created with BioRender.com. Second-generation rAdV vectors Second-generation...Second-generation rAdV vectors contain the genomic deletions of first-generation rAdV vectors, as well as the deletion...

...Viral Vectors AAV Vector Guide Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production...vitro factories for the new viral particles in the laboratory. The cell line used for rAAV vector production...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...used as viral vectors for gene therapy applications. This guide contains many viral vector-specific terms...adenovirus, read our Adenoviral Vector Guide . Recombinant AAV Vectors Wild-type AAVs have been modified...Plasmids required for rAAV vector production. Created with BioRender.com. AAV Vector Production Cloning Cloning...protocols, see Addgene’s Viral Vector Protocols . Figure 3: Overview of rAAV vector production. Created with...

...Chemogenetic Receptors: RASSLs The first chemogenetic receptors were based on G-protein coupled receptors (GPCRs...References Chemogenetic tools are actuators for specific cellular pathways, receptors, or ion channels targeted...Specifically, Receptors Activated Solely by Synthetic Ligands (RASSLs) based on κ-opiod receptors (Coward et...intracellular calcium stores. There are currently three Gq DREADDs based on human muscarinic receptors: hM1Dq, hM3Dq...muscarinic receptors, hM2Di and hM4Di, and a Gi DREADD based on the human κ-opoid receptor, termed KORD...turkey erythrocyte β-adrenergic receptor with a rat M3 muscarinic receptor. This DREADD was shown to have...DREADDs and their activity in neurons DREADD Receptor Effector Ligand Effect Outcome (in neurons) Reference...