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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,052 results for: sta

Showing: 1041 - 1052 of 1052 results

  1. Lentivirus ddPCR Titration

    Type
    Protocol
    ...from the publication Wang et al. (2018) . Before Starting Thaw the master mix, primers/probe mixes and samples...Heat-inactivated FBS L-alanyl-L-glutamine (or alternative stable glutamine such as glutaGRO, Corning 25-015-CI) ...the dilutions. RPP30 copies should be relatively constant across samples. In the RRE example below, 2-fold...below, the concentration of RPP30 positive droplets stays relatively even across samples (green). To increase...

  2. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...DNA fragments based on size. The procedure starts with standard agarose gel electrophoresis, which separates...efficient with lower % agarose gels, so you will want to stay in the 0.7-0.8% range if possible. You will want...

  3. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ... agarose using a commercially available kit or standard protocol. Anneal oligos The oligos should be resuspended...tube. Place tube in a thermocycler programmed to start at 95°C for 2 minutes. Then, gradually cool to 25...ratios (vector:insert) between 4:3 and 1:6 in a standard ligation reaction (ex. to ligate an annealed oligo...

  4. Fluorescence Titering Assay

    Type
    Protocol
    ... 10-013-CV L-alanyl-L-glutamine (or alternative stable glutamine such as glutaGRO, Corning 25-015-CI) ...: 10% v/v FBS and 4 mM L-alanyl-L-glutamine (or stable alternative, such as glutaGRO) To a 500 mL bottle... to 56 °C for 30 min. Considerations Before You Start The health of the target cell line is critical for...

  5. Gibson Assembly Protocol

    Type
    Protocol
    ...Get started with Gibson Assembly Cloning!...plasmids by designing primers to split an antibiotic resistance gene to effectively create an extra part, one...contiguous DNA fragment. Pro-Tip Add Extreme Thermostable Single-Stranded DNA-Binding protein (ET SSB)...

  6. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...the SV40 origin of replication use a HEK293 line stably expressing the SV40 large T antigen. For antibody-expressing...Epstein-Barr virus origin of replication use a HEK293 line stable expressing Epstein Barr nuclear antigen (EBNA).... saline solution, Millipore Sigma A6279 Before Starting Warm the DNA and working stock of PEI-MAX to room...

  8. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...entire construct. Is the primer an appropriate distance from the target? The primer should be a minimum...and make sure they are justifiable peaks. For instance, in the trace file below, you can see that just...

  9. Centrifugation

    Type
    Protocol
    ... out more protocols and videos to help you get started in the lab! Introduction A centrifuge is a common...balanced. Using the centrifuge in an unbalanced state can damage the centrifuge and be dangerous for the...

  10. AAV ddPCR Titration

    Type
    Protocol
    .... (2014) (Link opens in a new window) . Before Starting To reduce the risk of contaminating reagents we...0.5 2 50 Annealing/Extension 60 1 2 50 Signal Stabilization 98 10 2 1 Hold 4 ∞ 2 1 After PCR is complete...

  11. Protocol - How to Design Primers

    Type
    Protocol
    ...properties: Length of 18-24 bases 40-60% G/C content Start and end with 1-2 G/C pairs Melting temperature (...
Showing: 1041 - 1052 of 1052 results