We narrowed to 955 results for: ARC;
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TypeBlog Post...requirements. To solve this problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo...efficiency. Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it ... 3. Swarts, Daan C., et al. “Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that...
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Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post... red light instead of blue, which will allow researchers to independently control the activity of two .... Understanding the brain through optogenetics research Addgene: What are some of the big problems that...Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed Boyden's lab Sign ... -
Celebrate National Book Month with Addgene
TypeBlog Post...Inferior: How Science Got Women Wrong - and the New Research That's Rewriting the Story by Angela Saini "This...in what happens when scientists bring biases to research and don't collaborate with others to bring diverse... series, there is always another secret." - Marcy Patrick, Associate Director of Quality Control Jonathan... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles have some advantages...isolation protocol by Maguire and colleagues, other researchers have tried to modify it to improve yields. For... viral protocols and videos Find AAVs for your research ... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...systems for mammalian screens, which enables researchers to maximize the chance of transducing a only ...downstream of the apoB gene. Using this reporter, the researchers explore many aspects of ApoB-LP biology, including...these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as the eFRET sensors, ... -
DAP arrays for multiplex gene editing
TypeBlog Post... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array Qichen Yuan is a PhD... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver. Most types of biological...enough to engineer it to suit our purposes? In search of simple biological machines R bodies can repeatedly...environment. Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department of... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...around 100-fold. These improvements will allow researchers to better fine-tune the level of output gene ... Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA. Nature. 2005 Nov 24;438(7067):441...Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Cell. 2009 Jun 26;... -
15 Hot Plasmids from 2017
TypeBlog Post...undoubtedly constitute a valuable tool for the research community. You can find the AAV vectors for infecting...imaging of two biosensors simultaneously Researchers in the labs of Michael Lin and Ryohei Yasuda ...sodium/potassium pump ATP1A1 in addition to a researcher’s locus of interest. NHEJ- or HDR-induced mutations...Cre recombination. Each knockout includes unique barcodes and universal priming sites to ensure the collection... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...mutational abilities of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell...flexibility to address many different types of research questions. Are you ready to start EvolvR-ing your...Perez-Pinera. "The continuously evolving CRISPR barcoding toolbox." Genome biology 19.1 (2018): 143. PubMed...