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  1. Components of CRISPR/Cas9

    Type
    Blog Post
    ... that while this post is intended to provide a general overview of CRISPR components, new Cas9 variants...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...

  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...TagRFP to give a brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will ...set of proteins the green and red proteins are generally the most detectable and so should be used to tag...

  3. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...studying up before you begin the hiring process. Read Genevive Bjorn's "Ready, Set, Hire" on Nature Jobs for ...Gilbert: “Very interdisciplinary, with emphasis on genetic engineering, sequencing, and translation of results...

  4. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...helpful changes are endless.    Fig. 2: Blugene moves reagents from left to right as they use them...documents/Western%20Blotting.pdf  More resources on Addgene’s blog A Guide to Getting Started in Undergrad ...

  5. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...stellar resume and cover letter can be found on the Addgene blog.   3. Personal statement brilliance In addition...co-founder of STEAMid.     Additional resources on the Addgene blog Check out other tools and resources for early...

  7. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...Framework Learning to think like an immunologist, a geneticist, a physicist, etc., is an immense task (it takes...accessible to your audience.  More resources on the Addgene blog Intro to our Summer SciComm Series...

  9. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...to plan ahead.     Resources Resources on the Addgene blog The Basics of Western Blotting Find other ...out other Antibody 101 articles Resources on addgene.org Western Blot Protocol Western Blot Protocol Video...

  10. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...Twitter into a paper-finding-machine. There are four general types of accounts you can follow: Scientists: ...scientific literature. Additional resources on the Addgene blog Learn why scientists should be on Twitter...

  12. Common Injection Routes in Mice

    Type
    Blog Post
    ...Resources and references Additional resources on the Addgene blog Mouse Modeling Part 1 Mouse Modeling Part ...Popular Model Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression...

  13. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...more people to quit the lab. Resources on the Addgene Blog Read Joanne Kamen's Blog Series on Mentoring...Browse All of Our Career Posts Resources on the Addgene.org Find Plasmids for Your Research Learn About ...

  14. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ... based on using a thin plane (sheet) of light generated by sunlight to observe single gold particles with...).  These viral plasmids are available through Addgene.  The combination of LSFM with multiple tissue ...

  15. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter...Internet "trolls"   More resources on the Addgene blog:  Summer SciComm Series: Cognitive LoadIntro...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ... conventional antibodies, chemical dyes, and genetically encoded fluorescent proteins (Gambarotto, et ....7554/elife.46249  Additional resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence...

  17. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...initiation proteins to start their own replication. In general, these plasmids tend to be low copy number. Conversely.../doi.org/10.1093/emboj/20.24.7323 Resources at Addgene Read our blog post on the Origin of Replication...

  18. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ... a common source of conflict. For instance, at Addgene, there are two teams whose primary role is customer... Jan. 3, 2022.     Additional Resources on the Addgene blog Management for ScientistsTransferable Skills...

  19. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...equipment that you use in your current lab first. Generally, major equipment can be defined as equipment with...Additional Resources Find other career posts on the Addgene Blog! Learn more about Experimental Designs Consulting...

  20. Finding Your Science Policy Path

    Type
    Blog Post
    ...fellowship is that you are often vetted for your general scientific expertise and then once selected, you...Twitter at @Steph_Guerra Additional resource on the Addgene blog: Browse our blog posts on science career ...
Showing: 1081 - 1100 of 1138 results