1,183 results

Use this protocol to transfect mammalian cells with your plasmid of interest.

Follow this protocol using Iodixanol gradient ultracentrifugation to get pure adeno-associated virus (AAV) preps for use in your research.

Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.

Protocol for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more.

Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands.

Learn how to transform E. coli with your plasmid of interest.

Protocol for creating a glycerol stock, including additional tips for long-term storage.

Protocol - How to Ligate Plasmid DNA

How to purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ

Calculate your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol.

Protocol for Inoculating a Bacterial Culture

Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands.

Learn how to streak bacteria on an LB agar plate to obtain single colonies.

Protocols for plasmid cloning by PCR.

Get started with Gibson Assembly Cloning!

Modify your plasmid MCS or add short sequences using oligo overlap cloning.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Protocol for Ligation Independent Cloning