1,183 results

Description of Polymerase Chain Reaction with protocol, tips and FAQ

Tips on designing primers for PCR

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Best practices for performing sequence analysis of a plasmid

Learn how to add antibiotic to a plate after it's already been poured and solidified.

A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos.

Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli cells.

Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration.

Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration.

Follow this protocol to perform RNA extraction without a kit.

In this protocol you will learn the basics of how to weigh reagents.

These guidelines summarize the proper personal protective equipment (PPE) for BSL-1 and BSL-2 labs.

These lab safety guidelines provide steps to ensure you are working in BSL-1 and BSL-2 labs safely.

Learn about selecting the correct pipettor and pipette tip, how to dispense liquids, and how to handle the pipette.

Find step-by-step instructions on how to use a water bath in the lab.

In this protocol you will learn the basics of how to use a light microscope.

This protocol describes the basic steps for fixing and staining cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody.

Lab protocol to determine purity and concentration of recombinant antibodies using ready-to-use biosafe Coomassie stain and ImageJ software.

This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using Protein A or Protein G columns.

This protocol describes the basic steps for lysing cells, determining total protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting.