1,183 results

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and common uses.

Learn about using Optogenetics plasmids in your lab, including microbial opsins and optical switch systems.

Learn all about plasmid pooled libraries, how to amplify and use them, and what types of experiments can be done with a pooled library.

Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and common uses.

In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and explain common applications.

Learn about the basics of molecular biology, including molecular genetics, plasmids, sequencing, and more.

Learn about bacterial and eukaryotic promoters, including transcription and the transcription complex.

Find a list of universal sequencing primers and other common sequencing primers.

Learn about different molecular cloning techniques, focusing on seven common cloning methods.

Learn about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit and the MoClo Plant Parts Kit.

Not sure where to start? Need a quick reference? Addgene's eBooks are here to help!

A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations.

Use this protocol to prepare LB agar plates with antibiotic in your lab.

Protocol, tips, and FAQ for how to perform a restriction digest of plasmid DNA

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

Use this protocol to generate lentivirus

This protocol can be used to titer lentiviral preparations that confer antibiotic resistance.

Protocol that can be used to titer lentiviral preparations carrying a fluorescence marker.