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We narrowed to 404 results for: pho;

Showing: 51 - 60 of 404 results

  1. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...brightness, photostability, and most importantly, a photoswitching ability. This photoswitching refers to...exciting the fluorophore at peak wavelength can also decrease the rate of photobleaching. Interaction ...function in live cells. Fluorophore properties: The specific properties of the fluorophore, such as excitation...fluorescence quantum yield, the ratio of photons absorbed over the photons emitted. For example, DAPI is much...Ghods, R. (n.d.). Comparison of the Photobleaching and Photostability Traits of Alexa Fluor 568-and Fluorescein...ligands Figure 1: Example structures of fluorophores, fluorescent dyes, and fluorescent ligands. ...refers to the emission of light by a substance (fluorophore) that has absorbed light or electromagnetic radiation...

  2. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...image can be acquired more quickly. 2-Photon Microscopy 2-Photon Microscopy attempts to solve two drawbacks... leads to more rapid photobleaching and a reduction in signal intensity. 2-Photon microscopes restrict...can lead to photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which...University. No matter whether you are a sports photographer at the Super Bowl, a medical technologist taking...background. In addition to this, the use of multiple fluorophores can add a second layer of contrast, color contrast...further exploit the unique characteristics of fluorophores to block out of focus fluorescence from reaching...large number of optical slices are acquired all fluorophores throughout the sample are constantly exposed...

  3. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...molecule is excited by a high-energy photon and re-emits a lower-energy photon; no chemical reaction takes place... light Chemical reaction High-energy photons Kinetics of photon generation Slower Faster Cofactors...Higher Lower Background Lower Higher Photobleaching/phototoxicity Not susceptible Susceptible Subcellular...of energy from an excited donor fluorophore to an acceptor fluorophore, which then emits it as light. ...Wikimedia Commons. The North American firefly, Photinus pyralis, catalyzes a chemical reaction in its ... luciferase reporters aren’t susceptible to photobleaching — they’ll keep emitting light as long as their...sea pansy (Renilla reniformis), click beetle (Pyrophorus plagiophthalamus), deep-sea copepod (Gaussia ...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...non-radiatively from an excited molecular fluorophore (the donor) to another fluorophore (the acceptor) by means of ...the two fluorophores used are in close vicinity. Thus, the distance between the two fluorophores and their...yield of the donor (number of emitted photons per absorbed photons) and the extinction coefficient of the... of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore colours...microscopy (FLIM) method. Acceptor photobleaching method The acceptor photobleaching method is simple but limited...dipole-dipole coupling." The emission of the acceptor fluorophore can be measured using microscopy techniques. ...interactions within living cells. By coupling fluorophores to proteins, pioneers of this technique were...

  5. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...donor fluorophore to the acceptor fluorophore. The typical steps in FRET are: 1. Donor fluorophore excitation...excited fluorophore, FRET involves a non-radiative transfer of energy (i.e. no emitted photons) from the...absorption of a photon 2. Energy transfer from the excited donor directly to the acceptor fluorophore--think of... of it as a virtual photon 3. Relaxation of the acceptor fluorophore back to its ground state by emission...acceptor fluorophores must be compatible, such that the emission spectrum of the donor fluorophore overlaps...emission of a photon with wavelength specific to the acceptor In order for FRET to occur several constraints...with the excitation spectrum of the acceptor fluorophore. Otherwise the energy transferred from the donor...

  6. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...to identify a fluorophore, spectral flow cytometers use all detectors for all fluorophores. This allows...samples stained with a single fluorophore. They’re required for every fluorophore you use in your panel, whether...conventional flow cytometry, light emitted by a fluorophore is guided to a single detector using a series...flow cytometry uses one detector to detect one fluorophore and therefore one cellular marker. However, emission... emission spectra of certain fluorophores can “spill over” into detectors that are not the primary detector...detector for that fluorophore. For example, in figure 1, the FITC signal is spilling over into the primary...the full spectrum of light to distinguish one fluorophore from another. Instead of using a single (primary...

  7. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...overcome these woes with photoactivatable and photocleavable gRNAs. Photocleavable guides are generated by...modification is 3’ phosphorothioate linkage, where a sulfur replaces a nonbridging phosphate oxygen. Other ...light. The photoactivatable counterpart to this system utilizes a gRNA which is photocaged and unable ...switches. Stabilizing gRNA modifications  Sugar phosphate backbone modifications The recognition sequence... have been employed which modify the sugar or phosphate molecules of the RNA backbone to increase stability...coupling several of them together, for example phosphorothioates with 2’-O-methyl modifications, proved more... specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune response...

  8. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... conjugated to a fluorophore, not every marker will have a corresponding fluorophore-conjugated antibody...cases, fluorophore-conjugated antibodies may exist, but only with a limited selection of fluorophores. If... tag but not a fluorophore. In a separate step, cells are incubated with a fluorophore-conjugated streptavidin...wide range of fluorophores, allowing for great flexibility when it comes to fluorophore selection for ...common method is a cell surface label, where fluorophore-conjugated antibodies directly bind to epitopes...employ beyond simply incubating cells with single-fluorophore-conjugated antibodies. These methods can include...interaction (Figure 1a). This interaction brings the fluorophore in proximity to the target marker, allowing for...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...releases energy as a photon. The photon energy is transferred to the acceptor fluorophore, a circularly permuted...range of mitochondrially targeted photoactivatable and photoswitchable FPs that are activated by UV light...subpopulations within the same cell; by photoswitching PSmOrange and photoactivating PAGFP in separate subpopulations... fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1.... In the case of pHlash, the signal from the fluorophore is pH-dependent. Signal is simultaneously acquired...e43072. doi: 10.1371/journal.pone.0043072. Photoswitchable tools for spatial and temporal control of cell...Three years ago, Brian Kuhlman’s lab designed photoswitchable dimers by fusing the SsrA peptide with the ...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...improved expression of recombinant phosphoproteins Protein phosphorylation is one of the most abundant forms...questions surrounding serine phosphorylation by adding this phosphorylated amino acid to the genetic code...the original or improved phosphoprotein synthesis reagents. One phosphoserine (SP218) insertion was moderately...plasmids required to make singly or multiply phosphorylated proteins (B40 OTS and pCRT7-GFP). To demonstrate...activating kinase 1 (MEK1) with either one or two phosphoserine residues cotranslationally inserted in their...moderately enhanced while two phosphoserine insertions (SP218/SP222) was dramatically enhanced with the...Addgene’s information page for the Rinehart phosphoprotein system, which includes detailed protocols provided...
Showing: 51 - 60 of 404 results