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We narrowed to 111 results for: lentiviral dna vectors

Showing: 101 - 111 of 111 results

  1. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...communication. Viral vectors and the nervous system With the advent of viral vector technology, new methods... at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have been widely used... The AAV genome is a single-stranded DNA molecule and AAV vectors are replication deficient (i.e. they... low immunogenicity and cellular toxicity. AAV vectors can be equipped with strong promoters for high ... is often made Cre-dependent. If Cre-dependent vectors are used in transgenic animals that express Cre...that also express Cre. Another advantage of AAV vectors is that there are well-established protocols for...life cycle. Using AAV for neuronal tracing AAV vectors are the first choice of many neuroscientists, with...

  2. Lentiviral Vector Guide

    Type
    Guide
    ...Viral Vectors Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector...Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically ranges from... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...Second-generation lentiviral plasmids greatly improved the safety of lentiviral vector production by removing...between second- and third-generation lentiviral plasmids. Lentiviral Vector Production Cloning Cloning your...

  3. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...Science Guides Viral Vectors Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral...Much like lentiviral vectors, gamma-retroviral vectors integrate at random locations throughout the genome... for lentiviral vectors, but a similar method can be applied when using gamma-retroviral vectors. Gene...of Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...Gamma-Retroviral Vectors Vector Production Integration Pseudotyping Common Uses Safety Resources Plasmid ...

  4. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...My lab's vector of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, ...Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols...always make more AAV than I think I need. Unlike lentivirus, AAV requires a higher number of viral particles...are present in an AAV prep. AAV samples are first DNaseI digested to remove any residual AAV plasmid that... account for the dilution of the sample for the DNAseI digest. Use good sterile technique when handling...Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols Find AAV ...

  5. Plan Your Experiment

    Type
    Guide
    ...viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral vectors... in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. ...delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes... CRISPR plasmids . Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...don’t discuss CRISPR screens in this guide, lentiviral vectors are a common choice for conducting genome-wide.... Both lentiviral and AAV vectors are safe to use in the lab, but AAVs exhibits the lowest immunogenicity...

  6. Guide to Using Pooled Libraries

    Type
    Guide
    ...plasmid DNA can be delivered directly to the cells of interest. With others, notably pooled lentiviral plasmid...case, next-generation sequencing of the maxiprep DNA is recommended to verify that the library is complete...Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...delivering as virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells...sequencing allows for parallel sequencing of millions of DNA fragments simultaneously in a single reaction. As...genome-wide screening with CRISPR/Cas9 . Browse our Viral Vector resources . Check out our blog posts featuring ...libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

  7. CRISPR Guide

    Type
    Guide
    ...purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA. The ease of generating...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which... two formats: as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at...homology with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence...will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...strands of the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands are...

  8. Molecular Biology Reference

    Type
    Guide
    ...plasmid or vector DNA backbone generates a recombinant DNA molecule, which can be used to study DNA fragments...recombinant DNA, in which many identical DNA fragments can be generated. The combination of a DNA fragment... cloning of a DNA fragment. Commonly used cloning vectors include Gateway entry vectors and TOPO cloning...replication, DNA unwinds and the DNA polymerase enzyme binds to and migrates down the single stranded DNA adding... to copy DNA regions of interest. In vitro DNA replication requires the 4 nucleotides, a DNA polymerase...complementary DNA and acts as a starting point for the DNA polymerase. Thus to replicate a piece of DNA in vitro...translated from RNA, which is transcribed from DNA. DNA is comprised of 4 nucleotides or bases, adenine...

  9. Plasmid Collections

    Type
    Collection
    ...Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus... view all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Meyer 114193 pc DNA FRT TO- APP695 APP CMV Alzheimer's Aleksandra Radenovic 114194 pc DNA FRT TO- APPSwed...237005 VCP-HaloTag Vector VCP Halo CMV ALS Promega Corporation 237006 HaloTag-VCP Vector VCP Halo CMV ALS...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...ALS Promega Corporation 237148 NanoBRET Assay Vector, TREM2-HaloTag TREM2 Halo CMV Alzheimer's Promega ...Corporation 237172 EIF2AK2(252-551)-NanoLuc Fusion Vector EIF2AK2 NanoLuc CMV VWM disease Promega Corporation...Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 ...

  11. Immunology Research Plasmids and Resources

    Type
    Collection
    ...Gateway® Entry vectors from the labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE...histocompatibility complex, class II, DO alpha HLA-DNA, HLA-DZA, HLADZ HLA-DOB major histocompatibility ...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building... reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope...system that can be used to construct complex lentiviruses for modification of mammalian cells with a single...
Showing: 101 - 111 of 111 results