... we would recommend using a cDNA overexpression vector rather than going through all the troubleshooting...et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences from a given... CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find...

... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and ... Gradinaru Tools for Affinity Purification These AAV encode tools for affinity purification (which can...Heintz , Nectow , Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology...Name Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven...

... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our... all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection, including...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...our control AAV that express fluorescent proteins under general promoters. These AAV can be used to compare...different serotypes. Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrh10 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10... 1 Looger 45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP Cre dependent 9 Sanes 45186 AAV-EF1a-BbChT EF1a ...

...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with the...

... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, rg* GENIE 162379 pGP-AAV-syn-FLEX-...162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f...176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE...162374 pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...

...Viral Vector Packaging Service Penn Vector Core Transfer Viral Vector Packaging Service: Penn Vector Core...provide high-quality AAV vectors to the academic research community. The Penn Vector Core will produce viral-based...Addgene's Current AAV inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics...Optogenetics Baljit Khakh Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes ...Penn Vector Core and Addgene partnership to provide AAV viral preps, includes listing of transfer status...University of Pennsylvania Vector Core (Link opens in a new window) (Penn Vector Core) and Addgene have partnered...viral-based vectors for distribution via Addgene. For a full list of Addgene's viral vectors See Addgene's...

...Viral Vector Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics AAV Optogenetic...Optogenetics AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, ...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...filters below to find ready-to-use AAV preparations of optogenetics plasmids available in Addgene's collection...20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell... 28017 AAV-CAG-hChR2-H134R-tdTomato CAG ChR2/H134R tdTomato Constitutive rg* Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2...

...Guide Blog: Plasmids 101-Viral Vector Elements Viral Vectors 101 eBook Overview of Viral Plasmids Which Type... Adeno-associated Virus (AAV) Plasmids | Guide Adeno-associated viruses (AAVs) are commonly used for gene...List of viral vector plasmids available at Addgene and other related resources. Special...Collections Viral Plasmids Viral Plasmids and Resources You May Also Like... Addgene's Viral Service Biosafety Guide...for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses...the host genome. AAVs are limited by their small packaging capacity. Adenovirus Plasmids | Guide Adenoviruses...

... Viral Vector Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of... particular AAV prep, mostly likely due to one of the following reasons: Transfer plasmids with sequence...any viral vector. Addgene performs QC measures which verify the identity of the viral vector, but not .../mL for the AAV1, AAV5, AAV8, or AAV Retrograde serotypes and 2 x 10 12 GC/mL for the AAV2 serotype, as...and limited use label licenses (LULLs). Titer For AAV preps produced through our Packaged on Request service...the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids with a cargo that may reduce titer due to toxicity...guarantees above. We will have our expert viral vector production team carefully review your cargo/serotype...

...luciferase Paul Khavari 109395 EFS-Vector Firefly EF1α AAV-CRISPR knockout plasmid. Firefly luciferase is used...transfection Sidi Chen 109396 TBG-Vector Firefly TBG AAV-CRISPR knockout plasmid. Firefly luciferase is used...Collections Luciferase Plasmids Luciferase Plasmid Collection You may also like... Fluorescent Proteins Blog...luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids from 2016...luciferase reporter. These plasmids improve over the original STARR-seq plasmids by using the bacterial ORI...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...regions Joshua Mendell 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...

...ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type ... Plasmid Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may...Guide Viral Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Chemogenetics vs. Optogenetics...Chemogenetics Plasmids Browse, sort, or search this table to find chemogenetics plasmids in our collection...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Optogenetics Chemogenetics plasmids encode genetically engineered receptors that are activated by specific...collection. For example, search for "hM4D mCherry", "KORD AAV", "LMO", "Sternson", or other terms. Looking for ...

... using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer vector and used to generate...stable cell lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of... in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP...generate AAV particles (for details, see our AAV Guide ) ∼4.5 kb packaging limit (only compatible with...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...desired vector. In many cases, targeting oligos are synthesized, annealed, and inserted into plasmids containing...particular model organism. There may not be a perfect plasmid for your specific application, and in such cases...

... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids...Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...time. Single plasmids containing both the gRNA and a Cas protein act as an all-in-one vector, but their ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited...Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus, Worm, or Other. Whether the plasmid contains...

...ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter... Plasmid Collections Optogenetics Plasmids Optogenetics Plasmid Collection You may ...Guide Microbial Opsin Plasmids Optical Switch Plasmids Background Optogenetics plasmids encode proteins that...like... Optogenetics Guide AAV Viral Service Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent...Microbial Opsin Plasmids Browse, sort, or search the table below for the optogenetics plasmids for neuronal... top Optical Switch Plasmids Browse, sort, or search the table below for plasmids that use light to control...transcription", "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication Return...

... (blue) light switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use ...Zhang showed they could package LITEs in viral vectors and genetically target them to specific cell populations...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest...offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found here.) So...

...Capsids AAV Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are plasmids which...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations...Institute for Brain Science AAV Enhancer Collection Allen Institute for Brain Science AAV Enhancer Collection ...populations using systemic AAV delivery. The collection includes the top vectors with the highest specificity...Enhancer Plasmids The Allen Institute for Brain Science Enhancer Collection contains best-in-class AAV plasmids...Addgene ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled Population Region 230551 pAAV-AiE2638m-minBG-SYFP2...window) . Figure 1: Selection, prep, and testing of AAV enhancers in mice. Enhancers were selected from the...

...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...gene promoters C. elegans vector kit - Collection of plasmids from the Fire Lab L4440 -...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...

... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant... Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids provided by the foundation...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system...lab developed new fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of ... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...

...-driven viral vectors (AAV1) - and you can also find several other constructs in plasmid form. ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...JEDI-2p viral preps here (and here!)Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue... improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins...’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a...myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc...

...T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG...synthetic biology, and viral vector plasmids. Plasmid... Plasmid Collections Depositor Plasmid Collections Depositor Collections...Addgene's repository contains 158,555 plasmids, which includes plasmid collections from depositors. To help... you find it helpful! You May Also Like: Plasmid Kits Plasmid Special Collections Pooled Libraries Antibodies...Collection Rett Syndrome Plasmid Collection and Resource Center TREAT-AD Plasmid Collection Visualization... for Brain Science AAV Enhancer Collection Allen Institute for Cell Science Plasmid Collection New England...

...types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you about AAV components... discussed below. Overview of AAV Plasmid System Common Uses of AAV AAV is commonly used in optogenetics...Parts of an AAV Transfer Plasmid Video Web References Vector Biolabs: Introduction to AAV University of...Serotypes Pseudotyping AAV Variants References AAV Components The small (4.8 kb) ssDNA AAV genome consists of... the AAV life cycle; VP1, VP2, VP3 - capsid proteins). When constructing an AAV transfer plasmid, the ...and VA) mediate AAV replication. The transfer plasmid, Rep/Cap, and the helper plasmid are transfected...to find plasmids optimized for use in AAV, or containing Staphylococcus aureus (SaCas9) . AAV is also ...

...below for CRISPR plasmids for RNA editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI Publication...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...capabilities but are small enough to be packaged in AAV particles. Want more information on the wide variety...

...delivery of a plasmid. shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources ... backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression as well...argonaute, please search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene...pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...

...adenoviral vector constructs. The system consists of two types of plasmids: shuttle (or transfer) vectors and...and adenoviral vectors. Find vectors for this system on our adenovirus plasmid page. The transgene of interest...Questions (FAQ) about Adenoviral Vectors Are adenovirus and AAV different? Yes! AAV is a small single-stranded... Ad5-based vector Most common type of adenoviral vector pAdTrack Class of transfer vectors that contain...adenovirus types, and most adenoviral vectors are based on Ad5. Ad5-based vectors use the Coxsackie-Adenovirus ...adenovirus. Overview of Adenoviral Plasmid System AdEasy™ for Adenoviral Vector Construction AdEasy™, developed...adenoviral plasmid containing the adenoviral genes necessary for virus production. The shuttle vector and the...

... NGS Plasmid Validation: SeqwellHow to Keep a Lab Notebook for Bioinformatics AnalysesAAV Vector Quality...days, we would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while...while more recently we have moved to full plasmid sequencing at our facility using Illumina MiSeq Next Generation...lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing data collection...transform the raw sequencing data into a complete plasmid sequence that would be ready for our QC team to...With our outline of individual steps to automate plasmid sequence assembly, and possible solutions for each...up with our sequencing instrument (hundreds of plasmids each week!), we already planned to use cloud computing...

...drive expression. Different AAV serotypes can be used to target AAV vectors to specific cells of interest... For tissue specificity of AAV serotypes, see our AAV guide . FLEx Vectors are also used to achieve cell-specific...Want to know more about FLEx vectors? Read our Plasmids 101: FLEx Vectors blog! Download our chemogenetics... chemogenetics plasmids available through Addgene, check out our Chemogenetics Plasmid Collection . Figure...receptors, and a wide variety of AAV-encoding chemogenetics plasmids are available. Additionally, genetically...our collection of Ready-to-use AAV Preparations of Chemogenetic Plasmids ! Table 4. Common promoters in...expression. Depending on the experiment, an AAV-encoded chemogenetic plasmid may need to be targeted to specific...

...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 158,555 plasmids . We've...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE Plasmid Collection for Solute Carrier Proteins... Find deposited plasmids for specific disease research areas. Cancer Research Plasmids and Resource Michael... Syndrome Plasmid Collection and Resource Center Do you have suggestions for other plasmids that should...

...Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have...

...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ...protocol to transfect mammalian cells with your plasmid of interest. Protocols...General Transfection You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral ..., the plasmid should also be propagated in an endonuclease negative E. coli strain such as NEB stable....is prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 ...of HEK293T cells optimized for viral production (AAVpro or Lenti-X), but any HEK293T cell line should work...