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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 387 results for: nans

Showing: 151 - 180 of 387 results

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    ...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote...-limiting step for efficient transduction by recombinant adeno-associated virus vectors." Journal of virology...10. Wu, Jianqing, et al. "Self-complementary recombinant adeno-associated viral vectors: packaging capacity...

  3. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ...imaged with stimulated emission depletion (STED) nanoscopy. Scale bar, 2 μm. Inset shows comparison with ...& Bewersdorf, J. (2016). Two-colour live-cell nanoscale imaging of intracellular targets. Nature Communications... R. Y. (1998). Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells. Science,...

  4. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post
    ...light (~400 nm), the chromophore photoconverts predominantly to the anionic form, causing increased fluorescence...multilabel imaging and dual color fluorescence nanoscopy." Nature biotechnology 26.9 (2008): 1035-1040....fluorescent proteins for far-field fluorescence nanoscopy." Biophysical journal 95.6 (2008): 2989-2997. ...

  5. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...EcoFlex a great option for those interested in recombinant protein purification and metabolic engineering...expression, the system includes a toolkit to express recombinant proteins within the mitochondrial matrix.   ...localization and transport studies in HEK cells, recombinant protein purification in bacterial cells, and ...

  6. 27 Hot Plasmids from 2016

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    Blog Post
    ...expresses the λ Red recombinase enzymes, as well as a dominant-negative mutator allele of the E. coli MMR protein...and SnoopTag/SnoopCatcher The development of recombinant DNA technology has allowed molecular biologists...wine, fuels, pharmaceutical ingredients, and recombinant proteins. In order to keep production yields ...containing long homology arms and both auxotrophic and dominant selection markers, allowing for use with both ...

  7. Addgene Launches Partnership with IPI

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    Blog Post
    ...collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first collection, a set of integrin...sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access to IPI...

  8. Troubleshooting and Optimizing a Western Blot

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    Blog Post
    ...manufacturer. You can also consider using monoclonal or recombinant antibodies, which have much lower lot-to-lot ...point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal...enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly...

  9. Addgene's Top Blog Posts from 2020

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    Blog Post
    ... their reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein expression, and more. We ...Quantification Methods to Consider by Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification discussions...

  10. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS one..., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification: a comprehensive...

  13. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current...

  16. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives...multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry...

  17. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    Blog Post
    ...fragment, single-chain variable fragment (scFv), or a nanobody is one potential remedy for this problem within... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...

  18. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...how punctuation can be wildly funny as well as financially critical. Those who work on the publishing side...the right audiences and how open access affects financial models in information publication. Take a look...

  22. The Importance of High Titer for AAV Transductions

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    Blog Post
    ...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...

  23. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...

  24. Antibodies 101: Isotypes

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    Blog Post
    ...classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of ..., but it’s also found in tissues. IgG is the predominant isotype during a secondary immune response, which...

  25. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...their first protocol is available now. It is for recombinant expression of phosphoserine-including proteins...Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https:/...

  26. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for...export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation of partnerships...

  27. Antibodies 101: Secondary Antibodies

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    Blog Post
    ...The introduction of antibody fragments, such as nanobodies, has introduced new options for increasing signal... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...

  28. Addgene: The First Twenty Years

    Type
    Blog Post
    ... we could use the same principles to provide recombinant antibodies, another plasmid-based product that... In March 2022, we launched our ready-to-use recombinant antibodies service, part of the Neuroscience ...

  29. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...not a “Fab” fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide...conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have the highest tissue penetration...
Showing: 151 - 180 of 387 results