We narrowed to 389 results for: ARIA

1. Build Your CRISPR Vocabulary

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   ... is composed of identical repeat sequences and variable spacer sequences. The array is transcribed and...

2. What's the Best Way to Elute and Store Your Plasmid DNA?

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   ...Tris-EDTA (TE), just Tris buffer, or some other variations. Does it make a difference? Short answer: yes...

3. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   ...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for...

4. CRISPR 101: Drug discovery and CRISPR

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   ... line knock-ins of patient mutations which are variants of unknown significance – mutations with unclear...

5. Hot Plasmids and Viral Preps - July 2021

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   ...in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and reduced...

6. New CRISPR Web Resources and #12DaysofCRISPR Recap

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   ...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and...

7. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

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   ...Addgene, positively affect scientific research. As Maria Castro of University of Michigan says, “Addgene ...

8. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   ...components, and challenging target genomes, such as the malaria-causing Plasmodium falciparum. Throughout these...

9. Optogenetics Guide

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   ... its variants allow for inhibition of neurons using blue-green light. Mac Variants. Mac variants have ...Inhibitory (Hyperpolarizing) ChR Variants. Alternatively, ChR variants that inhibit neurons have been created... these variants result in the hyperpolarization of neurons. Examples of anion channel variants from other...thus inhibiting function of the neuron. NpHR Variants. Variants have been engineered with enhancements such... triggered by green-yellow light. Arch Variants. Arch variants have been developed with the following ...plasmids . Glossary of Microbial Opsin Variants Opsin Type Variant Description Peak Response Spectra (nm...Phobos Blue-shifted iC++ variant 467 Aurora Red-shifted anion-selective ReaChR variant 517 Halorhodopsins Jaws...

10. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    ...consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing...

11. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    ... aureochrome1 (VfAu1), led to functional BLADE variants, too. The team tested different linkers between...

12. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    ...system could be used to deliver or measure new variables. The sky is really the limit for this emerging...

13. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    ...per cell per color to achieve the highest color variations. Adjust the plasmid concentrations or AAV titer...

14. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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    ...variety of science concepts. From inheritance and variation, to growth and development, and response to environment...

15. CRISPR Challenges: Standardization and Homology Directed Repair

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    ...Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as...

16. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    ... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire...

17. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    ...metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory ON/OFF master switches...

18. Extracurricular Activities for a Strong Science Career Path

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    ...future regulations impact your work? What design variables need consideration? Enrich your knowledge and ...

19. What Good Citizenship Can Do for Reproducibility in Science

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    ...never completely eliminate the risk that a minor variation in experimental conditions will derail a research...

20. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ...versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that were...

21. Creating accessible biology activities in schools with BioBits

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    ...Additional Resources on the Addgene Blog Find planarian tools for teaching developmental biology Use the...

22. "Build Your Plasmid": The Game - Play to Teach

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    ...Additional Resources on the Addgene Blog Find planarian tools for teaching developmental biology Learn...

23. Visualizing Translation at the Single Molecule Level

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    ...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...

24. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    ...background. In other cases, YFG or one of its mutant variants will need to be overexpressed or knocked down....

25. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    ...Nanobodies are a small (~15 kDa), single monomeric variable heavy chain antibody domain.  How do these components...

26. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...in vivo, the non-immunogenic violet-excited GFP variant Vex. To understand the impact of this gene editing...

27. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ... virus is produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables ...

28. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    ...expression vectors containing various spike protein variants are also available. Plasmids are continually added...

29. Viral Vectors 101: Viral Vector Elements

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    ..., we are here to help with resources from AAV variables that matter to adenovirus production tips. For...

30. Antibodies 101: Monoclonal Antibodies

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    ...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080...

31. 15 Years of Addgene: The Top 15 Plasmids

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    ...expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting...

32. Why and how to start studying chromatin (with Addgene's help)

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    ...that histone genes frequently have many different variant names, not all of which can be listed here. If ...

33. Multiplex Genome Editing with CRISPR-Cpf1

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    ...the gRNAs and Cas9. Co-transfections can lead to variable expression levels due to differences in copy number...

34. Top Requested Lentivirus and AAV of 2016

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    ...challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast to generate Cas9-expressing...

35. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    ...patient mutations using SDM can help determine if a variant is causal for a given disease. CRISPR has made ...

36. CRISPR 101: Non-Homologous End Joining

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    ...Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...

37. CRISPR Guide

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    ...binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9...LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter...Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' ...use, and there are several to choose from. Many variations of Cas9 exist, along with other Cas enzymes like... PAM-flexible or PAMless Cas9s. SpCas9-derived variants with non-NGG PAM sequences include: xCas9 - NG...plant genome engineering. Read more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a larger editing window...

38. Antibodies 101: Conjugation

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    ... methods for antibody conjugation can be quite variable from antibody to antibody or batch to batch. For...

39. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    .... So far, there have been thirty GNB1 missense variants identified from 58 individuals with GNB1 disorder...

40. Antibodies 101: Secondary Antibodies

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    ...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...

41. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    ...Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo and have used it to detect...

42. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...transgenes of interest. Furthermore, fluorophore variants (for example green and red fluorescent proteins...

43. RaPID Detection of RNA-protein Interactions

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    ...disease. RaPID can also be used to test how RNA variants associated with human disease affect protein binding...

44. RANbodies: Reporter Nanobody Fusions

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    ...animal derived product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies...

45. Human Germline Editing Using CRISPR

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    ...CRISPR Topic Page Read about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources...

46. Choosing the B(right)est Fluorescent Protein: Photostability

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    ...higher excitation power, the photostability of the variants is comparable (lower panel of figure 1). To avoid...

47. CRISPRainbow and Genome Visualization

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    ... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...

48. Developing Lab Management Software for Biology

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    ...-true tools We use Python, Django, Apache, and MariaDB. Using tried-and-true tools means less time getting...

49. Gendered Innovations: Why Does Sex of the Cell Matter?

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    ... requires that sex be included as a biological variable in all NIH-funded research. Janine Clayton, Director...

50. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ...Our paper shows we can easily make orthogonal variation. The first one worked in each case. Sometimes ...