We narrowed to 438 results for: ERG

1. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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   ... most significant being the usage of a strong detergent to permeabilize cells rather than to rely on nuclei...

2. What's the Best Way to Elute and Store Your Plasmid DNA?

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   ... can lead to mutations in single stranded DNA undergoing replication.   DNase on the other hand can digest...

3. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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   ...pivotal role in spontaneous swimming. References Arrenberg, Aristides B., Filippo Del Bene, and Herwig Baier...

4. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

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   ...As an undergraduate student at Northeastern University, I am required to complete a six month work experience...

5. CRISPR 101: Drug discovery and CRISPR

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   ...identified if it isn’t known. If a strong candidate emerges, downstream optimization is performed to take the...

6. Oh, The Places You Can Go: Careers in Science Communication - Product Development

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   ...Pharmacology, I taught a couple of lectures in the undergraduate pharmacology course, like one or two lectures...

7. Fluorescent Proteins 101: Fluorescent Biosensors

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   ...The sensor component is usually a protein that undergoes a conformational change in response to the molecule...

8. Addgene at the ASM Microbe 2016 Conference

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   ...well as being the Director of the Great Lakes Bioenergy Research Center and a fellow Rhodobacter enthusiast...

9. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   ...Though I attended an engineering school as an undergraduate, I never fooled myself into thinking I was an...

10. Advice for Choosing a Research Project

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    ...determine how you spend a good chuck of your mental energy and time. The research project also serves as a...

11. The Stingy Scientist: How the Baby Gel Box Was Born

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    ...environmentally conscious friends who had me thinking about energy conservation even before it was a popular topic...

12. Hot Plasmids and Viral Preps - September 2021

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    ...stronger promoter and different biochemical secondary energy sources to increase protein yields from 36 μg/mL...

13. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    ...Over the past several months, we’ve seen the emergence of several SARS-CoV-2 variants coincide with increased...

14. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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    ...function is inactivated by the separation of its synergistic domains. This paper demonstrated that the migration...

15. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    ..., Broucque F, Blouin V, Moullier P, Ayuso E, Vandenberghe LH (2017) AAV-ID: A Rapid and Robust Assay for...

16. New and Upcoming Viral Vectors - Spring 2019

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    ...example, the mDlx enhancer restricts expression to GABAergic interneurons. These vectors can also be used as...

17. Summer SciComm Series: Master of SciComm

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    ...when I grew up until I was all but finished my undergraduate degree (and I hope I’m not alone in this sentiment...

18. RUBY-Red Siliques

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    ...usage of antibiotics which may contribute to the emergence of antibiotic resistant disease. (RUBY-Red: You...

19. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    ...variables. The sky is really the limit for this emerging technology! Resources and References References...

20. Science Careers: Unruly Interests Feed Many Paths

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    ... – a career in the sciences is more like an intergalactic network. Which planet will you visit? Where ...

21. 10 Great Guest Posts We're Thankful For

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    ...blogger Tessa Cressy from the labs of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School...

22. Using Addgene's New Viral Service for Your Research

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    ...optogenetic tools coming soon). The viral preparations undergo rigorous quality control testing at Addgene meaning...

23. Plasmids 101: TOPO Cloning

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    ...digests double stranded DNA at this sequence. The energy from this breakage is stored as a covalent bond...

24. Don’t Wait To Launch Your Health Science Startup

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    ...think of?  Your mind is likely drawn to the Mark Zuckerbergs and Evan Spiegels of the world – individuals ...

25. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    ...6 days of growth, wild-type animals that have undergone SEC excision are screened based on the loss of...

26. Advice for Moving Into Sales After Your Science Postdoc

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    ... protocols, courses taken during my PhD and Undergraduate sections and brought in more focus on the list...

27. Hot Plasmids: FRET-Based Biosensors

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    ...the field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors. Perhaps...

28. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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    ...volunteers are extremely diverse. Some of them are undergraduates or PhD students who are just starting their...

29. "Build Your Plasmid": The Game - Play to Teach

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    ...lentivirus-based ex-vivo gene therapy’ strategies to undergraduates, however you may use it and modify it for non-commercial...

30. Scientists Map the SARS-CoV-2-Human Interaction Network

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    ...SARS-CoV-2 plasmids (Note: The Nsp6 construct is undergoing QC and will be available shortly. Sign up for...

31. Celebrate National Book Month with Addgene

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    ...literally changing my life. Though I have an undergraduate degree in English, I had lapsed. Baker’s novel...

32. New and Upcoming Viral Vectors - September 2019

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    ...The Dlx element directs expression in forebrain GABAergic interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry...

33. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    ... to answer the question of whether firing of serotonergic neurons (measured using a red calcium sensor...

34. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    ...have been developed, such as the Forster resonance energy transfer (FRET)-based executioner caspase reporters...

35. Summer SciComm Series: A PhD in Science Communication

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    ...the intel back to my shyer classmates. For my undergraduate degree, I majored in human anatomy, but I also...

36. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    ...Alzheimer’s disease mutation in basal forebrain cholinergic neurons derived from iPSCs. From Rohn et al. ...

37. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    ... Martin, W. D., Terry, F. E., Roberts, B. J., Rosenberg, A. S., & De Groot, A. S. (2022). In silico Immunogenicity...

38. Components of CRISPR/Cas9

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    ...center of the protein. Upon target binding, Cas9 undergoes a conformational change that positions the nuclease...

39. Addgene Welcomes John Doench to the Advisory Board

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    ... here! Doench majored in history during his undergraduate work at Hamilton College. When asked about what...

40. Advice for Scientists Starting a Lab

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    ...differently or the same as your adviser did? Tom Ellis merges the influence from his two PhD advisers who were...

41. Finding and Joining Your Dream Lab

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    ...abilities. You have intense periods of focus and energy for your work. You won't always be in the zone,...

42. Sweating the Small Stuff: Details in the Lab

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    ...Addgene’s blog A Guide to Getting Started in Undergrad Research Early Career Researcher Toolbox  8 Tactics...

43. GPCRs: How Do They Work and How Do We Study Them?

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    ...protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on ...

44. Plasmids 101: Inducible Promoters

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    ...lactose or lactose analog IPTG, the lac repressor undergoes a conformational change that removes it from lacO...

45. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    ...Figure 1: Illustration of SARS-CoV-2 from Philippa Steinberg for the IGI.   What SARS-CoV-2 plasmids from...

46. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    ...transfer and was discovered in 1946 by Joshua Lederberg and Edward Tatum when they observed genetic recombination...

47. 15 Years of Addgene: The Top 15 Plasmids

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    ...pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab and contains an envelope protein for producing...

48. AAV Q&A with Tim Miles

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    ... AAV-BR1. We recommend you keep an eye out for emerging new capsids in this area.   Q8: On dense labeling...

49. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    ...plot should indicate where the two populations diverge. The smear plot! The dreaded smear - fear not!...

50. Viral Vectors 101: Viral Applications

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    ...experiments? Let’s unpack the myriad number of emerging viral applications and give you some recommendations...