We narrowed to 400 results for: pho;

1. New Norepinephrine Indicators: nLightG and nLightR

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   ...experiments, and nLightG was successfully trialed in two-photon in vivo experiments. nLight indicators are more...

2. Future of Research Conference - Remarkable Opening Session

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   ...The keynote speaker, Dr. Henry Bourne (shown in photo), has been speaking out and writing about issues...

3. Fostering communication in remote collaborations

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   ...Having virtual face-to-face meetings as opposed to phone calls most closely replicates how you’d interact...

4. Embracing Serendipity: A Crucial Element in the PhD Journey

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   ...notebook at all times (or use the notes app on your phone) to capture fleeting thoughts and connections that...

5. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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   ...required.)  DailyCalcs Science Calculator app for iPhone Reagent and Data Repositories These links can ...

6. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

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   ...- no pipettes were harmed in the making of this photo!  Here, I was also given a mixture of support and...

7. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

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   ...we’d love to see you show it off! To share your photo, tag @Addgene and #happydepositor on Twitter. All...

8. Addgene at the ASM Microbe 2016 Conference

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   ...had plenty of wonderfully nerdy art for sale, and photos of the Agar Art competition entries were displayed...

9. Choosing a Good Mentor for Scientists

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   ... lab experiences and will be willing to talk by phone. Don’t use email to get information if you can help...

10. History of CRISPR Cas - A tale of survival and evolution

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    ... A. J Bacteriol. 1987 Dec; 169 (12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James...

11. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    ...scFvs) (Inbar et al., 1972) are fused to mature fluorophores, and can bind and light up epitope tagged proteins...

12. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    ... Any longer and the cells may start to show morphological abnormalities due to the excessive amount of...

13. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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    ...protocols, ABRC staff are available via e-mail and phone to answer questions and provide support. Growing...

14. In Vivo Biotinylation of Bacterial Fusion Proteins

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    ...biotinylated protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050-084; 0.2 μg/...

15. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    ...progresses.  In From Academic Solos to Industrial Symphonies, an article well worth reading on this topic,...

16. Summer SciComm Series: Master of SciComm

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    ... A scicomm group project on the Leith river. Photo courtesy of Leonardo Ramos.  I enrolled into...

17. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    ...antibody that has your reporter (in this case a fluorophore) directly attached to it. (B) Another solution...

18. Plasmids 101: TOPO Cloning

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    ...digest, or sequencing. Pro tips Do not add 5’ phosphates to your PCR primers; you need that free hydroxyl...

19. New and Upcoming Viral Vectors - December 2019

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    ...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons...

20. The Strength of Story Telling

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    ...Beijing, talking to people about taking beautiful photographs of their studies, reporting on amazing women ...

21. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    ... into XP genes and other genes involved in photosensitivity in melanocytes with the goal of understanding...

22. Mentoring for Scientists: I Have a Mentor, What Now?

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    ... prefer to communicate between meetings (email, phone, etc.)? Make agendas for your meetings, take informal...

23. Improving Transduction Efficiency with Exosome AAVs

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    ...intravitreal injection to transduce bipolar cells and photoreceptors (Wassmer et al., 2017), and exo-AAV8 expressing...

24. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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    ...Plasmids expressing fluorescent proteins and a new two-photon laser scanning microscope will allow Tsakanova ...

25. Creating accessible biology activities in schools with BioBits

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    ... way into high school classrooms, like gel electrophoresis to separate DNA or transforming cells with ...

26. "Build Your Plasmid": The Game - Play to Teach

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    ...many of them focused more on social media or the phones hiding under their desks than listening to me. ...

27. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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    ...different parts of the poster. Conferences, such as Photonics Online Meetup, hosted their poster session on ...

28. 25 Work From Home Tips for Scientists

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    ...team lunches, a virtual soup lunch, and sharing photos of our pets. #SocialDistancing may send a wrong...

29. Science Career Options

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    ...Documentary Director Parasitology Botanist Nature Photographer/Artist Ecologist     Computer and writing...

30. R Bodies: Membrane-Rupturing Microscopic Tools

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    ...Defensive extrusive ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are...

31. Addgene Welcomes John Doench to the Advisory Board

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    ...Doench explained: “I took a microbiology class my sophomore year and it was the first time that I was in a...

32. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...individual fluorescent protein (brightness, photostability, and so on; see the previous blog post for ...

33. Plasmids 101: Mammalian Vectors

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    ... and we will be covering this ever-versatile fluorophore in a later post, so stay tuned! Transient transfection...

34. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    ...physical methods in the lab. Chemicals like calcium phosphate and diethylaminoehtyl (DEAE)-dextra neutralize...

35. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    ...this by changing the voltage for the particular fluorophore prior to recording data. Increasing the voltage...

36. Clear and Accessible Writing

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    ...chose this alt text: "The left panel is a profile photo of an iodixanol gradient after ultracentrifugation...

37. GPCRs: How Do They Work and How Do We Study Them?

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    ...ligands, including hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades...

38. A Deep Dive into BioBuilder

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    ...the Importance of Assembly Standards Synthetic Photobiology: Optogentics for E. coli Browse All Synthetic...

39. 3 Challenges in Plant Synthetic Biology

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    ...better understand developmental, metabolic, and photosynthetic processes. Often the tools developed for plant...

40. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...which is necessary for effective STED), and be photostable over a broad pH range. The four biometric probes...

41. Visualizing Protein Turnover In Situ

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    ...Which is best for me? Learn how small molecule fluorophores can be used for cell labeling Check out other...

42. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...their own research. Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been recognized ...

43. Summer SciComm: Preprints

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    ... CERN — it's a new age of accelerating science! Photo courtesy of Henry Mühlpfordt, under a CC BY-SA license...

44. Summer SciComm Series: Cognitive Load

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    ... the slide deck (or reminding them to put their phones away), or maybe I was assuming something was intuitive...

45. Transferable Skills Guide: Public Speaking

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    ...Public speaking is not just one of the most common phobias–it’s a key part of being a scientist and an incredibly...

46. Addgene: The First Twenty Years

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    ...popular movie in theaters, the Razr was the hottest phone on the market, and two new websites, Facebook and...

47. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...expression of transgenes of interest. Furthermore, fluorophore variants (for example green and red fluorescent...

48. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ...al disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations...

49. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    ...FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning...

50. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    ...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...