...vapors. Workflow Timeline Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody incubation Day 4...with 96-well plates 1–10 µL single channel pipette 2–20 µL single channel pipette 20–200 µL single channel...serial dilutions of the purified antigen as follows: 2 ng/µL : Add 100 µL of 20 ng/µL stock into 900 µL PBS...microfuge tube and vortex. 1 ng/µL : Add 450 µL of 2 ng/µL stock into 450 uL PBS in a microfuge tube and...37 °C for 30 min , or overnight at 4 °C . Section 2: Block the plate Prepare the wash buffer (0.05% Tween...incubate on a microplate shaker set to 400 rpm for 2 h at room temperature or overnight at 4 °C . Section...

.... Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed... switches for pathogen detection In 2014, as postdoctoral fellows with Jim Collins and Peng Yin at the...

...out for help so you can get the support you need. 2) Prepare an agenda for every meeting Supervisors are...the needs of other people. Some students and postdoctoral fellows are hesitant about expressing their ...

...Bachelor's degree Generally a short-term position (1-2 years) Often a gap-year position taken to transition...teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc) Getting more scientific...scientific and professional training after earning a doctorate Considered a "stepping stone" position to become...

...- Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression...pp. 7913–7923 Hoi et al. : Chemistry & Biology, October 2013, Vol. 20 No. 10, pp.1296-304 Kogure et al....

...) or the American Chemical Society (ACS) are just 2 examples of organizations that hold large, annual ... students today is that many graduate and post-doctoral programs lack any formal or mandatory professional...

...15.4%) 46 (59%) of these students were in the last 2 years of their degree. Students filled in a pre-program...linkedln.       Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical School, where...

...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen.... R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer, J., Winchester...