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Showing: 101 - 120 of 393 results

  2. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...ladders, the 100 bp ladder works well for both agarose and native polyacrylamide gels. Get Tips on Verifying... to Analyze Your Restriction Digests Make Your Agarose Gel Other Resources Read about Gel Stains on ...

  3. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...impressive science-themed holiday decorations yet again. This year, we had two different categories: one...his Christmas tree! It is that time of the year again: miniature #Christmas tree goes up at home!This ...

  4. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...Court’s decision to ban gene patents in a case against Myriad, an organization called Free the Data launched...of 304 open access publishers. By the time the magazine went to press, no less than 157 of those journals...

  5. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...Senior Director of Biology, is helping us fight against FOP in two ways. First, Addgene is distributing...plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston...

  8. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ... antibodies drive an effective immune response against a pathogen. The Fc domain creates a link between...Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor targets. Nat Med. 2000 Apr;6(4):443-6. doi...

  9. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...partial loss of an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging...packaging. To prevent ITR loss, it’s best to propagate AAV constructs in recombination-deficient bacterial...

  10. RUBY-Red Siliques

    Type
    Blog Post
    ...applications. But some techniques require you to select against the presence of a transgene instead, which can’...are currently used in these situations to select against CRISPR positive plants, but screening these can...

  11. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...THE RESULTS DISCUSSED BELOW Biologists are going gaga over the newest gene-editing protein - a DNA-cleaving...prokaryotes, and some of these proteins protect against invading DNA. In 2014, Swarts et al. characterized...

  12. Plasmid Cloning by PCR

    Type
    Blog Post
    ...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

  13. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...mutations and truncations that can make vector propagation and sequencing a headache. How do I maintain ...integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth culture...

  14. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...lost for an age) fight in the Napoleonic Wars and against something even more sinister. 1000 pages long. ...reads, some are hard. Some I’ll never think about again, some I loved so much I’ll force them on everyone...

  16. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...experiment by simply running it unchanged over and over again, and the same goes for your workflow. Next time ...mistake, instead of silently vowing never to do that again (I’ve been there!), think of ways you could change...

  18. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...achieve my purpose? Should I go over this slide again? Say, 'Oh, I forgot to mention X, so let's quickly... freezes and then crashes. You’re muted on Zoom again.  These things don’t just happen to you or me (though...

  19. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...for every assay. Antibodies that are generated against a linear antigen tend to work very well for applications...compare the staining patterns of multiple antibodies against the same target; tagged protein strategies that...
Showing: 101 - 120 of 393 results