We narrowed to 398 results for: AGA
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TypeBlog Post...scientists delivered some spectacular photos once again this year! All winners receive an Addgene care package...(@ChiTownBio) and one of our favorite twitter #agarart accounts, Petri dish Picasso (@PetriPicasso) came...
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Congrats to the 2019 Deck The Lab Winners
TypeBlog Post...winners of the 2019 contest. Agar art For the second year in a row, agar art is one of the winning decorations... -
DIY DNA Ladders from Penn State University
TypeBlog Post...ladders, the 100 bp ladder works well for both agarose and native polyacrylamide gels. Get Tips on Verifying... to Analyze Your Restriction Digests Make Your Agarose Gel Other Resources Read about Gel Stains on ... -
Congratulations, Deck The Lab winners!
TypeBlog Post...impressive science-themed holiday decorations yet again. This year, we had two different categories: one...his Christmas tree! It is that time of the year again: miniature #Christmas tree goes up at home!This ... -
Top 10 Open Science Developments of 2013
TypeBlog Post...Court’s decision to ban gene patents in a case against Myriad, an organization called Free the Data launched...of 304 open access publishers. By the time the magazine went to press, no less than 157 of those journals... -
Antibodies 101: Fc Effector Functions
TypeBlog Post... antibodies drive an effective immune response against a pathogen. The Fc domain creates a link between...Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor targets. Nat Med. 2000 Apr;6(4):443-6. doi... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post...plasmid and the insert. By using the “Simulate agarose gel” option with the desktop version of Snapgene...more enzymes. Click here to learn how to run an agarose gel We’ve worked with Snapgene to update our... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog Post... fraction of readers cover the same ground again and again as they don’t save the insights they glean ... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post...Senior Director of Biology, is helping us fight against FOP in two ways. First, Addgene is distributing...plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...partial loss of an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging...packaging. To prevent ITR loss, it’s best to propagate AAV constructs in recombination-deficient bacterial... -
RUBY-Red Siliques
TypeBlog Post...applications. But some techniques require you to select against the presence of a transgene instead, which can’...are currently used in these situations to select against CRISPR positive plants, but screening these can... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...THE RESULTS DISCUSSED BELOW Biologists are going gaga over the newest gene-editing protein - a DNA-cleaving...prokaryotes, and some of these proteins protect against invading DNA. In 2014, Swarts et al. characterized... -
Plasmid Cloning by PCR
TypeBlog Post...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post...mutations and truncations that can make vector propagation and sequencing a headache. How do I maintain ...integrity and prevent unwanted mutations? During propagation, mutants rapidly replicate in a growth culture... -
Celebrate National Book Month with Addgene
TypeBlog Post...lost for an age) fight in the Napoleonic Wars and against something even more sinister. 1000 pages long. ...reads, some are hard. Some I’ll never think about again, some I loved so much I’ll force them on everyone... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...F, Clouaire T, Leung JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM Genes Dev. ...small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...experiment by simply running it unchanged over and over again, and the same goes for your workflow. Next time ...mistake, instead of silently vowing never to do that again (I’ve been there!), think of ways you could change... -
Plasmids 101: Yeast Vectors
TypeBlog Post...each one requires different elements for vector propagation. This post, along with a future companion post...yeast plasmid) allows for 50+ copies to stably propagate per cell. The copy number of these vectors can... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...achieve my purpose? Should I go over this slide again? Say, 'Oh, I forgot to mention X, so let's quickly... freezes and then crashes. You’re muted on Zoom again. These things don’t just happen to you or me (though... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post... Fred Hutch website, social channels and Hutch magazine. Getting pulled in all of these different directions...anything good. And I will never write anything good again.’ ” For Susan, one of the most satisfying parts ...