Plasmids 101: Cre-lox
Type
Blog Post
... independently of any other accessory proteins or co-factors, thus allowing for broad applications in ...
We narrowed to 184 results for: CO
-
-
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...control over when Cas9 activity is blocked. Hence, co-opting optogenetic techniques allows greater precision... LOV2 from A. Sativa AcrIIA4 anti-CRISPR protein, co-expressed with Cas9 or dCas9 Repression of gene editing... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...present on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid ...Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875... -
Human Germline Editing Using CRISPR
TypeBlog Post...caveat of mosaicism. To circumvent this problem, they co-injected CRISPR machinery with sperm into M phase... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...detect protein-protein interactions (yeast-two hybrid, Co-IP, BioID), and that’s just to name a few. But what... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...for TRIM-Away delivery. For small cell populations, co-microinjection of TRIM21 protein and the target antibody... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...ZFN and TALEN, can used to engineer knockin mice. Co-injection of a targeting vector containing homologous... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...organizations like Just-a-Start, the Northeastern co-op program, and with other intern positions. We have... -
Antibodies 101: Affinity Reagents
TypeBlog Post...assembling protein nanostructures. Diabodies readily co-crystalize, a major advantage over antibodies (Kwon... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...structural component of bones and teeth. It is an enzyme co-factor, a crosslinking agent, a signaling ion, and... -
To Each HIS Own
TypeBlog Post...present in most production systems and can bind and co-elute with the protein of interest (Bornhorst 2000... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...engineered to be electrically excitable. The mutants are co-expressed with a photoconvertible fluorescent protein... -
Adenovirus Guide
TypeGuide...into the adenoviral backbone plasmid. One can also co-transform standard BJ5183 with supercoiled pAdEasy...series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest.... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...AAV belongs to the family Parvoviridae and requires co-infection of helper viruses like adenovirus for productive... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...Masterclass in science communication. The National Co-Ordinating Centre for Public Engagement (NCCPE) provides... -
CRISPR in the Clinic
TypeBlog Post...the cataracts phenotype could be rescued by zygote co-injection of Cas9 and a single-guide RNA targeting... -
6 Tips for Grant Writing
TypeBlog Post...contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No matter what facet... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid DNA and lead to degradation... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...three Nobel Prize winners, one NIH Director and a co-founder of BGI-China presenting, it’s no wonder it... -
Genome-wide Screening Using CRISPR
TypeBlog Post...’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of wild-type Cas9 and a gRNA generates a...