... 8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a ...genes in mammalian cells include: SunTag system — co-expression of epitope-tagged dCas9 and antibody-activator...two-component system (Figure 9C) SAM activators — co-expression of dCas9-VP64 with a modified scaffold...non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome...dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen...other Cas enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex...