We narrowed to 186 results for: Coli

1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   ...University of Oregon created mTFP1 by transforming E. coli with a gene library of approximately 484 variants...

2. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

   Type

   Blog Post

   ...called BLADE (blue light-inducible AraC dimers in E. coli) that turns expression on and off with blue light...

3. 10 Great Guest Posts We're Thankful For

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   Blog Post

   ...proteins. She also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...

4. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

   Type

   Blog Post

   ... my days (and often my nights :D) engineering E. coli to produce biofuels in Pamela Silver’s Lab (plasmid...

5. How to Deposit Your Plasmids with Addgene

   Type

   Blog Post

   ...distribute your bacterial strains if they: are E. coli that can be used under BSL1 conditions were generated...you to produce large amounts of your insert in E. coli, then this is a Bacterial Expression vector. If ...

6. When Fidelity Matters: A frank discussion about ligase fidelity

   Type

   Blog Post

   ...thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase." Journal of bacteriology 173.16 (1991...Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate." Molecular...

7. Lentivirus Production

   Type

   Protocol

   ...also be propagated in an endonuclease negative E. coli strain such as NEB stable. Make a mixture of a total...

8. Plasmids 101: NGS Quality Control for Pooled Libraries

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   Blog Post

   ... Library amplification seems straightforward: E. coli bacteria are transformed with the library DNA, they...

9. History of CRISPR Cas - A tale of survival and evolution

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   Blog Post

   ...CRISPR sequences were initially discovered in E. coli in 1987 (1), the concept that these clustered repeat...

10. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post

    ...you're currently a synthetic biologist engineering E. coli metabolism, maybe you’ll be able to apply your knowledge...

11. A Deep Dive into BioBuilder

    Type

    Blog Post

    ...Standards Synthetic Photobiology: Optogentics for E. coli Browse All Synthetic Biology Blog Posts Resources...

12. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    ...Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other blog posts from our Plasmids 101 series...

13. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    ...not work. Check the plates! Streak on them an E. coli  strain known to be sensitive to that antibiotic...

14. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

    ...visualized in vivo using ultrasound in Escherichia coli and Salmonella Typhimurium. This work demonstrates...

15. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    ...robust system of homologous recombination found in E. coli, SLIC is a cheap, standardized, and rapid multi-...

16. Hot Plasmids - February 2022

    Type

    Blog Post

    ...20, 30, 40, 50, 60, 80 and 100 kD proteins in E. coli, either individually (~20,000 lanes/50 mLs) or through...

17. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    ...construct plasmids for use in model organisms such as E. coli and yeast. Because this approach uses standardized...

18. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    ...function. RNAi is commonly achieved by feeding E. coli expressing dsRNA to C. elegans. These dsRNAs target...

19. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    ... are linked to the regulatory networks within E. coli host cells. Image from Nielson and Voigt...

20. Viral Production

    Type

    Collection

    ...propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids are typically prepared using...