Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 443 results for: ENA

Showing: 101 - 120 of 443 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...nucleases and TALENs fused to epigenetic modifiers enabled epigenetic modifications at a user-specified locus...mammalian cells. Co-expression markers EGFP and PuroR enable sorting and selection of transduced cells. Grant...Gersbach CA (2017) CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory...https://doi.org/10.1093/nar/gkw159 Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler...

  2. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...products and use temperature to denature and renature the dsDNA. Upon renaturation, you will generate DNA duplexes...cells are growing so slowly, there isn’t much maintenance involved. Also it was really fun to find individual...

  3. Deck the Lab 2022!

    Type
    Blog Post
    ...                     A post shared by Xi Chen (@seleena78) The lab has officially welcomed the Christmas...giant paper mache Krampus performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities ...                     A post shared by Xi Chen (@seleena78)   Here’s how to enter: Post your picture or...

  4. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens in naïve T cells. More broadly,...lentiviral gRNA expression vector (pRDA_526) that (1) enables efficient knockout (KO) of genes in immune cells...also includes pLX_305_Ovalbumin, a plasmid that enables introduction of the model antigen, Ovalbumin, into...

  5. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...university partners quickly assessed how to more readily enable research. Often “normal” timelines that could be...resource from the Structural Genomics Consortium enables science to determine the three dimensional structures... proteins.  The RESOLUTE Consortium collection enables research on solute carriers (SLCs) for medical ...

  6. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...ways in which we promote our mission and work to enable researchers around the world. We take this responsibility...initiatives as opposed to fundraising.   Aside from enabling us to make all decisions with the goal of  benefiting... well. We work every day to do a better job of enabling more scientists in labs all over the world to ...

  8. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing (Chen et al., 2023). AAV-BI30, developed...S., Meltzer, J. C., Buzhdygan, T., Cheng, M. J., Sena-Esteves, M., Bennett, R. E., Sullivan, T. P., Razmpour...by engineering capsid–receptor interactions that enable crossing of the blood–brain barrier. PLoS Biology...carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral...Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...Tool    mCyRFP1: Novel fluorescent protein that enables imaging of two biosensors simultaneously   Researchers...between a protein and a tag, such as an NLS or NES, enables control over localization. Inserting PhoCl between... to the many plant studies that are sure to be enabled by this toolkit. For examples of the toolkit in...layer of regulation to genome engineering that enables new applications, such as reversing the effects...

  11. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...sharing, computational reproducibility, and tools to enable scientific reproducibility. This workshop was well...one at a scientific conference could potentially enable reproducibility practices being adopted by a wider...the group, lessons learned, and to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional...

  12. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...Fab fragments can be used as a block in similar scenarios. By applying unconjugated Fab fragments that are...site for conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have the highest...which antibody format to use for your specific scenario.    References and resources References Bates...

  13. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...frequently and recent advances in AAV technology have enabled us to generate new and refined forms of capsids...This characteristic makes AAV vectors especially amenable to correcting disease-associated mutations. AAV...deeper understanding of disease pathogenesis will enable robust therapeutic outcomes. In contrast to other...

  14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...can be calibrated to these different conditions, enabling the use of a given timer in many experimental ...protein expression dynamics in a synthetic circuit (Saxena et al.) In a general sense, FP timers can be used... 19478145. PubMed Central PMCID: PMC2712795. 6. Saxena, Pratik, et al. "A programmable synthetic lineage-control...

  15. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...Using these reagents and a series of heating (denaturing) and cooling (annealing) steps Taq polymerase...specifics of the 3 basic steps of a PCR reaction:  Denaturation- To amplify DNA, the two strands of the template... Steps in PCR reaction   This process of denaturation, annealing and extension is repeated 25-35 times...

  16. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ... Recent improvements in computer modeling have enabled researchers to begin discovering and designing ...signaling pathway. Its major appeal is that it enables the simple construction of vectors to study the... PMID: 24651010. 3. Ledford, Heidi. "The ras renaissance." Nature 520.7547 (2015): 278-280. PubMed PMID...

  17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...considered when using this method:     Cells must be amenable to transfection or viral transduction     Appropriate...studies in experimental systems that may not be amenable to plasmid based methods. One of the most common... "Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro...

  19. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...Principal Investigator meeting of the NSF EDGE (Enabling Discovery Through Genomic Tools) Program and I...the NSF program directors, this program should “enable the ability to study life’s innovations from anywhere...has created an automated tank for feeding and maintenance that had the audience gasping. Animal husbandry...

  20. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... provide a modern and fully supported API that enables development of custom integrations with other tools.... As illustrated in this video the integration enables RSpace users to search for protocols in protocols.io.... These integrations utilize an interface that enables users to conveniently select and deposit individual...
Showing: 101 - 120 of 443 results