Hot Plasmids - October 2020
Type
Blog Post
...expressed in the neuron, the FingRs bind to the endogenous synaptic proteins allowing them to localize to...
We narrowed to 145 results for: ENDOG
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Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...pseudotyped with a mix of VSV-G and the baboon endogenous retrovirus Rless glycoprotein (BaEVRless) had... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...your protein of interest. TIR1 interacts with endogenous Skp1 and Cul3 proteins to form a SCF E3 ubiquitin... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...biomolecules that are not proteins) or would perturb the endogenous state that you want to measure (overexpressing... -
Plasmids 101: FLEx Vectors
TypeBlog Post...mutations and truncations in the absence of the endogenous gene. Spatio-temporal expression of optogenetic... -
Of Myc and Men
TypeBlog Post...impossible if you wanted to distinguish between endogenous and exogenous versions of the same target. In... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...CAPTURE CAPTURE 2.0 Biotinylation enzyme BirA Endogenous biotin ligases Expression system Stable cell... -
Hot Plasmids: Summer 2025
TypeBlog Post...) for easy visualization. A cleavage site for endogenous BACE1 protease allows VAMP2-At to remain on the... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...Western blotting and PCR, depending on the level of endogenous expression. We highly recommend the use of an... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging in Human Cells Check Out the Michael... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post... level observed will be dependent on both the endogenous promoter and the 3’ UTR of the locus of interest... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...from a template molecule, which is usually an endogenously available sister chromatid, though exogenous... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...then tested the RESCUE system’s ability to edit endogenous transcripts finding up to 42% editing efficiency... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post... this using Cre-Lox mouse models in which the endogenous MECP2 gene was disrupted and silenced by a Lox-Stop... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...efficiency is correlated with codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...Agrobacterium lacking the GUS gene since plants can have endogenous GUS-like activity (Hu et al., 1990). We commonly... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system." Proceedings of the National... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post...The Promoter Region – Let's Go! Plasmids for Endogenous Gene Tagging in Human Cells Resources on addgene.org... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...new effector (Abudayyeh et al., 2016). In the endogenous CRISPR/Cas9 system, Cas9 uses both a tracrRNA... -
CRISPR Activation: A Practical Guide
TypeBlog Post...light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11:198–200 . https...