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We narrowed to 395 results for: ICL

Showing: 101 - 120 of 395 results

  1. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...expression or localization in cells, or even single-particle tracking of the bound complex. There are so many...EBFP2(1-10) can be used with GFP(11)). Some of the articles linked below only provide one fragment of the ...Protein Tags Browse the table below, and click the Article link to find all the plasmids from the associated...associated paper:   Color Base FP Description Article PI Blue EBFP2 EBFP2(1-10) and Capri(1-10) for ...

  2. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ... will highlight four tools for finding journal articles related to your actual interests while also staying...publication date, a relevance score, and type of article. Now, search results display a bar graph of the...PubMed entry are great places to look for more articles on your topic of interest.  Best uses Accessing... including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There...

  3. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...suppressors to the accelerator and brake pedal of a vehicle, respectively. In the case of a mutation that turns...oncogene), this is analogous to the accelerator of a vehicle being stuck down. Similarly, a mutation that turns...the same result – uncontrolled movement of the vehicle or, in the case of cancer, uncontrolled cell proliferation...non-cancerous cells, such as those within hair follicles and lining the digestive system, causing discomfort...

  4. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...lentivirus, AAV requires a higher number of viral particles for efficient gene transfer. I figure it’s better...alone. If yield is important, or if the viral particles of the AAV serotype you’re working with are primarily...ethanol and dry ice bath to release the virus particles from the cells. Cell lysates are then treated ...quantifies the number of genome-containing viral particles that are present in an AAV prep. AAV samples are...

  5. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... polyomavirus virus-like particles (MPyV VLPs ). This artificial nanoparticle can protect proteins from...

  6. Viral Vector Guides and Plasmids

    Type
    Collection
    ...more viruses. In this way, viruses are excellent vehicles to deliver genetic cargo into cells. To create...

  7. Antibody Plasmid Collection

    Type
    Collection
    ... express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy- and light-chain...

  9. TALEN Guide

    Type
    Collection
    ...days. In an open access Nucleic Acids Research article that came out in the spring of 2011, the groups...

  10. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells...’s lab developed a lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins...binding protein for packaging into a lentivirus-like particle. Read the paper in Nucleic Acids Research Find...

  11. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral...genomic screens Using lentivirus as the delivery vehicle, Neville Sanjana, also of the Broad Institute, ...M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs: Using CRISPR/Cas to...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter so be... expression. The plasmids associated with this article can be obtained through Addgene: Rapamycin-inducible...course of gene activation using this system in the article cited below. Scientists can obtain dCas9-trCIB1...trCIB1, CRY2PHR-p65, and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and...TOP2 constructs; see Supplementary Note 3 in the article for more info) are available. Furthermore, there...

  13. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here’s what you’ll find in this article: CRISPR-based editing in organoid Biosensors for...

  15. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here's what you'll find in this article: An improved cell-free system to examine biosynthetic...

  16. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...types of biological motion (whether endocytosis, vesicle trafficking, or muscle contractions) are produced...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...

  17. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...a brief overview see Addgene’s AAV guide. AAV particles are relatively small (20 nm) in size which makes...different AAV vectors into a brain region, the AAV particles will infect neurons at different ratios, making...and may be a form of transcytosis where the AAV particle is taken up on one side of the cell and released...cell. While there is always a risk that viral particles will diffuse long distances within the extracellular...

  19. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...too large to be effectively packaged into AAV particles for delivery into mammalian cells, especially ...mRNA to facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA...SEND for gene transfer with pseudotyped viral particles, expanding options for CRISPR or other gene delivery...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...stock, followed by resuspension of the collected particles in a smaller volume. Always remove packaging cell...reagents typically enhance the adsorption of a virus particle to a target cell by reducing the repulsive electrostatic...an accurate count of the number of infectious particles in a given viral stock will give you a sense of...
Showing: 101 - 120 of 395 results