We narrowed to 395 results for: ICL

...more viruses. In this way, viruses are excellent vehicles to deliver genetic cargo into cells. To create...

...components necessary to produce infectious viral particles have been split into multiple plasmids for safety...

... express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy- and light-chain...

...Institute for Cell Science has produced the first publicly available collection (Link opens in a new window...

...days. In an open access Nucleic Acids Research article that came out in the spring of 2011, the groups...

...information that will be contained in the viral particle (i.e., the AAV vector genome), flanked by two ...

...ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells...’s lab developed a lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins...binding protein for packaging into a lentivirus-like particle. Read the paper in Nucleic Acids Research Find...

...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral...genomic screens Using lentivirus as the delivery vehicle, Neville Sanjana, also of the Broad Institute, ...M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs: Using CRISPR/Cas to...

...of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter so be... expression. The plasmids associated with this article can be obtained through Addgene: Rapamycin-inducible...course of gene activation using this system in the article cited below. Scientists can obtain dCas9-trCIB1...trCIB1, CRY2PHR-p65, and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and...TOP2 constructs; see Supplementary Note 3 in the article for more info) are available. Furthermore, there...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here’s what you’ll find in this article: CRISPR-based editing in organoid Biosensors for...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here's what you'll find in this article: An improved cell-free system to examine biosynthetic...

...the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down. ...100°C. They published their finding in a bioRxiv article and in PNAS. Two other thermostable Cas9 enzymes...

...types of biological motion (whether endocytosis, vesicle trafficking, or muscle contractions) are produced...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...

...a brief overview see Addgene’s AAV guide. AAV particles are relatively small (20 nm) in size which makes...different AAV vectors into a brain region, the AAV particles will infect neurons at different ratios, making...and may be a form of transcytosis where the AAV particle is taken up on one side of the cell and released...cell. While there is always a risk that viral particles will diffuse long distances within the extracellular...

...more viral particles. Eventually the cell lyses releasing the newly formed viral particles that can infect...are packaged into replication-incompetent phage particles with assistance from a ‘helper’ phage prior to...

...too large to be effectively packaged into AAV particles for delivery into mammalian cells, especially ...mRNA to facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA...SEND for gene transfer with pseudotyped viral particles, expanding options for CRISPR or other gene delivery...

...to update our post. From Mice to Men When this article was originally written, the main focus was on editing...technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to the liver...disease approaches targeting cholesterol, these particles were an obvious delivery choice. In sum, CRISPR...

...stock, followed by resuspension of the collected particles in a smaller volume. Always remove packaging cell...reagents typically enhance the adsorption of a virus particle to a target cell by reducing the repulsive electrostatic...an accurate count of the number of infectious particles in a given viral stock will give you a sense of...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... the Biggiephage clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1...

...components necessary to produce an infectious viral particle (a virion) are divided among multiple plasmids...