...too large to be effectively packaged into AAV particles for delivery into mammalian cells, especially ...mRNA to facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA...SEND for gene transfer with pseudotyped viral particles, expanding options for CRISPR or other gene delivery...

...to update our post. From Mice to Men When this article was originally written, the main focus was on editing...technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to the liver...disease approaches targeting cholesterol, these particles were an obvious delivery choice. In sum, CRISPR...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... the Biggiephage clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1...

...stock, followed by resuspension of the collected particles in a smaller volume. Always remove packaging cell...reagents typically enhance the adsorption of a virus particle to a target cell by reducing the repulsive electrostatic...an accurate count of the number of infectious particles in a given viral stock will give you a sense of...

... modified in the golgi and then transported in vesicles via the secretory pathway to their subcellular...cycle, proteins may be transported through the vesicles to the lysosome where they are finally degraded... hope you find the resources discussed in this article useful for your next localization experiment and...

...dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle cargoes to FP... et al. "Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by ...

...examples: experiment or analysis. In the rest of this article, we'll explain the thinking behind the tool, its...do this internally, but they are not available publicly. Furthermore, we did not find any tools which ...the How To Guide. Conclusion CrispyCrunch is now publicly-available for high-throughput design and analysis...

...highlight some of the reagents that have been made publicly available through Addgene.  What makes CRISPR... Individual gRNAs are designed in silico using publicly available gRNA design software and synthesized...derivatives of Cas9. Several CRISPR libraries are publicly available through Addgene, with more being added...

...genomic, or DNA fragments from agarose).    In this article, we will go through the basics of four methods ...milk-like appearance. To make DNA stick to the glass particles in glass milk, gel slices are first placed in ...stick to the glass. After binding DNA, the glass particles are pelleted by centrifugation and washed before...

...1,2), and cell types used for packaging viral particles (6), can influence vector performance. Open science...citable DOIs through DataCite before being made publicly available through the Data Hub. These minimum ...of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent its occurrence...

...checked out Gal's blog, you should! He's got great articles and insight on the newest tools and techniques...fluorescent microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide Note: A. ...Max Juchheim contributed to the writing of this article. ...

...preps in the repository through our Hot Plasmids articles. Today, it's time for another edition of Hot Plasmids...and targeting components to reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for...   Figure 2: Modular features of DIRECTED particles where the fusion and targeting components are ...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...