We narrowed to 396 results for: ICL
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TypeCollection...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
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Synthetic Biology - Networks and Gene Regulation
TypeCollection...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available... -
CRISPR Plasmids - RNA Editing
TypeCollection...capabilities but are small enough to be packaged in AAV particles. Want more information on the wide variety of ... -
Ginkgo Bioworks COVID-19 Collection
TypeCollection..., we have linked to collections of open-access articles, protocols, and other resource collections related... -
AAV Viral Preps
TypeCollection...distributed as 100 µL solutions of purified viral particles that are suitable for in vivo use. Minimum titers... -
Fluorescent Protein Guide: In Vivo Imaging
TypeCollection...mCardinal 604/659 18 All mCardinal plasmids from article mNeptune 600/650 13.4 pcDNA3-mNeptune-N E2-Crimson... -
What's New in CRISPR - December 2019
TypeBlog Post...ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells...’s lab developed a lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins...binding protein for packaging into a lentivirus-like particle. Read the paper in Nucleic Acids Research Find... -
Zinc Finger Consortium: OPEN Reagents
TypeCollection...acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral...genomic screens Using lentivirus as the delivery vehicle, Neville Sanjana, also of the Broad Institute, ...M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs: Using CRISPR/Cas to... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter so be... expression. The plasmids associated with this article can be obtained through Addgene: Rapamycin-inducible...course of gene activation using this system in the article cited below. Scientists can obtain dCas9-trCIB1...trCIB1, CRY2PHR-p65, and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and...TOP2 constructs; see Supplementary Note 3 in the article for more info) are available. Furthermore, there... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here’s what you’ll find in this article: CRISPR-based editing in organoid Biosensors for... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here's what you'll find in this article: An improved cell-free system to examine biosynthetic... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down. ...100°C. They published their finding in a bioRxiv article and in PNAS. Two other thermostable Cas9 enzymes... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...types of biological motion (whether endocytosis, vesicle trafficking, or muscle contractions) are produced...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ... -
Synthetic Biology - Overview
TypeCollection...USA SynBio initiative Recent Synthetic Biology Articles - Peccoud lab social media tracker Synthetic Biology... -
Using AAV for Neuronal Tracing
TypeBlog Post...a brief overview see Addgene’s AAV guide. AAV particles are relatively small (20 nm) in size which makes...different AAV vectors into a brain region, the AAV particles will infect neurons at different ratios, making...and may be a form of transcytosis where the AAV particle is taken up on one side of the cell and released...cell. While there is always a risk that viral particles will diffuse long distances within the extracellular... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...more viral particles. Eventually the cell lyses releasing the newly formed viral particles that can infect...are packaged into replication-incompetent phage particles with assistance from a ‘helper’ phage prior to... -
What's New in CRISPR - November 2021
TypeBlog Post...too large to be effectively packaged into AAV particles for delivery into mammalian cells, especially ...mRNA to facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA...SEND for gene transfer with pseudotyped viral particles, expanding options for CRISPR or other gene delivery... -
CRISPR in the Clinic
TypeBlog Post...to update our post. From Mice to Men When this article was originally written, the main focus was on editing...technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to the liver...disease approaches targeting cholesterol, these particles were an obvious delivery choice. In sum, CRISPR... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... the Biggiephage clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1...