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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 920 results for: IRE;

Showing: 101 - 120 of 920 results

  1. Adenovirus Guide

    Type
    Guide
    ...different purposes. The pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP ...recombinant adenoviral vector production that contain IRES-GFP. Pseudotyping The production of viral vectors...got their name, but they are not related. AAVs require the presence of adenoviral genes E1, E4, E2a and... available inside of the adenoviral capsid and directly correlates to the size of the DNA removed from... of genetic cargo. Production of HCAdV vectors requires using an additional adenoviral helper virus (HV...system, recombination between both plasmids occurs directly in the HEK293 packaging cell line, engineered ...of the genome adjacent to the left ITR that is required for viral genome packaging. In helper viruses,...

  2. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of...

  4. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...CRISPR Activate and Interfere technologies also indirectly modify local chromatin state. Want more information...

  5. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... is an A->G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it...

  9. Plasmid Collections

    Type
    Collection
    ... are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...

  10. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...wiki entries describing enzyme activity, PAM requirements, experimental considerations, and more, created...

  13. AAV Packaged on Request

    Type
    Collection
    ...needs of many researchers, scientists sometimes require preps beyond our current in-stock capabilities....

  14. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...emission of a photon, the acceptor molecule is not required to be fluorescent. A variety of organic dyes ...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein abundance, size, and ...

  16. Open Enzyme Collection

    Type
    Collection
    ... new functions through genetic engineering and directed evolution. More information about the collection...

  18. Lentivirus Plasmids

    Type
    Collection
    ...lentivirus: these viruses are based on HIV-1, which may require additional lab biosafety procedures. Additionally...

  19. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... fluorescent protein downstream, separated by an IRES element. You did a lot of work! But let’s take a...-thi-1 or thi1 Mutation in thiamine metabolism Requires exogenous thiamine for growth ara disruption...leuB β-isopropyl malate dehydrogenase inactivated Requires exogenous leucine source for growth proAB mutation...mutation in proline biosynthesis pathway Requires exogenous proline source for growth rpsL Mutation ...This strain is endA+, so column-based DNA preps require an additional wash step. F- glnV44 recA13 mcrB ...
Showing: 101 - 120 of 920 results