We narrowed to 108 results for: K-Ras protein

...cancer.     References 1. Russell, David W., and Roll K. Hirata. "Human gene targeting by viral vectors." ...devised methods to deliver CRISPR components as ribonucleoproteins (RNPs) that can be supplied separately from.... PubMed Central PMCID: PMC4752446. 5. Asuri, Prashanth, et al. "Directed evolution of adeno-associated...28646206. PubMed Central PMCID: PMC5482879. 7. Bak, Rasmus O., and Matthew H. Porteus. "CRISPR-mediated integration...

...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ..., or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...applicability of base editing through protein engineering and protein delivery. Nature Communications . 8... by anti-CRISPR proteins. Nature , 526 (7571), 136–139. PMID: 26416740 Bravo, J. P. K., Liu, M., Hibshman...see Cas3 for more details), while type V-K systems use a type V-K Cas effector, Cas12k. No matter the system...

...., Fujimoto, A., Mimura, K., English, J. G., Liu, J., Inoue, K. I., Kumata, K., Seki, C., … Minamimoto...transmembrane proteins that bind a specific ligand. Ligand binding then activates G-proteins to modulate...Na + , K + influx Neuronal activation Björefeldt et al., 2024 LMO11 GeNL_SS ChRmine CFz Na + , K + influx...10.1073/pnas.0700293104 PMID: 17360345 Berglund, K., Clissold, K., Li, H. E., Wen, L., Park, S. Y., Gleixner..., H., Urban, D. J., White, K. L., Rittiner, J. E., Crowley, N. A., Pleil, K. E., Mazzone, C. M., Mosier...DREADDs), like RASSLs, are based on engineered G-protein coupled receptors, but unlike RASSLs, show insensitivity...Chemogenetic Receptors: RASSLs The first chemogenetic receptors were based on G-protein coupled receptors (...

...release proteins from cellular membranes using light. light-induced protein dissociation using protein A fragments...photoreceptors to create protein systems controlled by light. These "photoswitchable" proteins offer exquisite ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...Inhibits protein function by reversibly sequestering targets into large, optically assembled protein architectures...transcription using engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage...pair to the target protein, and the other to the membrane where the target protein is to be sequestered...associate and cage a protein in the dark, while dissociate and activate the protein in the light ~400 (...

... pol in trans Precursor protein containing reverse transcriptase and integrase components. Envelope plasmid... vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein with broad tropism used to pseudotype...infectivity VSV-G (vesicular stomatitis virus G protein) is the most common envelope gene, due to a wide... Cichutek, K. (1997). Pseudotyping of murine leukemia virus with the envelope glycoproteins of HIV generates...RNA packaging signal; recognized by nucleocapsid proteins and essential for efficient viral packaging. cPPT...Packaging plasmid gag in trans Precursor structural protein of the retroviral particle containing matrix, capsid...viral vectors (or viruses) using viral envelope proteins from different species. Used to alter infectivity...

...fluorescence complementation experiments to analyze protein-protein interactions in plants. MoClo-YTK Yeast Expression..., synthetic terminators, protein purification tags and fluorescence proteins for use in E. coli for a ...plasmids including backbones, fluorescent proteins, protein tags, and signal peptides, for use in a range...transcription units for protein secretion in yeasts S. cerevisiae and P. pastoris (a.k.a. K. phaffii ). POMBOX...MoClo-YTK toolkit for protein expression and secretion in P. pastoris (a.k.a. K. phaffii ). GoldenPiCS...example, a promoter, a 5' UTR, a signal peptide, a protein-coding gene, and a terminator, as in Figure 1)....optimize secretion and cell surface display of proteins of interest. Yeast Secrete and Detect Yeast Expression...

...the instructions to make every protein within an organism. Before proteins can be translated, the DNA is...RNA (mRNA), which is then translated into proteins. Proteins are made up of amino acids. Each amino acid... from two individual isolates, the K-12 strain and the B strain. K-12 has led to the common lab strains...driven by that promoter. Fluorescent Protein Plasmids , Luciferase Plasmids Viral Plasmids These plasmids...discoveries led to the conclusion that genes encode proteins and reside on chromosomes, which are composed ...in the central dogma of molecular biology, that proteins are translated from RNA, which is transcribed ...translating the information encoded by DNA and RNA into proteins. DNA is comprised of four nucleotide bases: adenine...

...the Rev protein binds. Psi (Ѱ) in cis RNA packaging signal; recognized by nucleocapsid proteins and essential..., G., Lee, H. K., Bae, T., Park, S., Kim, Y. J., Lee, S., Park, J., Bae, S., & Hur, J. K. (2021). CReVIS-Seq... pol in trans Precursor protein containing reverse transcriptase and integrase components. rev in trans... vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein with broad tropism used to pseudotype...infectivity) VSV-G (vesicular stomatitis virus G protein) is the most common envelope gene in all systems...third-generation systems do not require the HIV protein Tat to activate the promoter on the transfer plasmid...Packaging plasmid gag in trans Precursor structural protein of the lentiviral particle containing matrix, capsid...