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We narrowed to 469 results for: LARGE

Showing: 101 - 120 of 469 results

  1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...site in the genome you are trying to target play a large role in Cas9 activity and efficiency (Sander and... structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They continued to...

  2. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...sequence, or if you want to amplify sequences from a large genome organisms like mouse or human. There are ...difficulties in amplifying a template, particularly from a large genome, you can recheck your primer design or optimize...

  6. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ..., gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases...frequency of both indels at each locus, as well as large deletions. They detected fragment deletion with ...

  7. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... to choose from that sequence preferences must largely be ignored. Created with BioRender.com. ...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets...

  8. Finding Your Science Policy Path

    Type
    Blog Post
    ...new way through the integration of science with a large set of other societal pressures including economic...p” policy is non-governmental work happening at large organizations who have to develop policies for their...policy to combat sexual harassment in academia or a large company who decides how they are going pursue regulatory...what better place to do that than at the nation’s largest integrated healthcare system? Defining your why...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...this latest work, the team designed and screened a large library of iGluSnFR3 mutants, identifying the two... making it ideal for video recordings of large populations of synapses and deep-brain fiber photometry...edits — single-base changes, small deletions, and large insertions up to 12 kb — with efficiencies between...somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making...

  10. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...previously-reported technique for precisely integrating large DNA sequences into a genome (Anzalone et al., 2022...deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nature Biotechnology... (2024). Efficient site-specific integration of large genes in mammalian cells via continuously evolved...

  11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...input to an RSM drives expression of a distinct large serine recombinase that recognizes its own attB ...high copy, ColE1 origin of replication), contains large serine recombinase genes downstream of inducible...faster or more efficiently than others. Multiple large serine recombinases have been described in the literature...

  13. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...the advent of CRISPR/Cas9 technology, inserting large tags into a precise location in the genome is still...fact. Later, when we transfect the cells, these large regions of homology enable us to effectively utilize...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...affinity reagents is their size. Antibodies are large enough that their bulk can be a barrier at times...difficult to source, may not be readily available for a large number of targets, and can require specific conjugates...

  15. To Each HIS Own

    Type
    Blog Post
    ...Moks 1987). These tags, however, tended to be very large and often required the incorporation of a cleavage...M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale affinity purification of human insulin-like...

  16. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...genome edits, but it is effective at wiping out large regions of DNA and has anti-viral application potential...facts – PAM requirement: AAG or TTC  Best for: very large deletions.   Cas12a – The staggered cutter  Originally...

  17. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...strategy designed by synthetic biologists to perform large scale, one-tube cloning reactions. In this blog,...this sounds really neat — but when is this type of large-scale assembly useful, you may ask? You might be...put, it assembles individual DNA components into larger units or circuits with functional relevance following...MoClo enables individual parts to be assembled into larger functional units which are transcribed together...translational output, etc., which can support a larger cellular engineering goal, several of which are...

  18. CRISPR 101 eBook...en español

    Type
    Blog Post
    ... 101: Una herramienta de edición  Addgene has a large international audience, including working with distributors...

  19. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...heavy machinery. But not all engineering is at a large scale — some is done on much smaller equipment on...generation. To evaluate improved properties across large mutant libraries, high-throughput screening techniques... Innovation (IPI) provides information on their large-scale recombinant antibody production platform. ...depth is antibody directed evolution. This is a larger process of gradually changing and selecting the...

  20. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ... some of the limitations of viruses. TEs have a large payload, up to 100 kB with Sleeping Beauty and piggyBac...payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, ...transposons to allow for the targeted delivery of large DNA cargos (>100 kB) to specific locations in the...
Showing: 101 - 120 of 469 results