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In Category

We narrowed to 256 results for: OVA

Showing: 101 - 120 of 256 results

  2. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...support from the Brain Research Through Advancing Innovative Neurotechnologies (BRAIN) Initiative Cell Census...Atlas will foster collaboration, progress, and innovation by allowing scientists to find and utilize reliable...

  3. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ... can result in the retention of introns or the removal of exons. Disrupting promoter or transcription ...) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large genomic fragment. (D) Knock-in...

  4. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, ...

  5. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...postdoc positions, including AstraZeneca, Pfizer, Novartis, Biogen, Amgen, New England Biolabs, Regeneron...pre-approved project. Other companies, such as Novartis, have specific web sites for postdoc listings,...

  7. Control AAV Preps

    Type
    Collection
    ...produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory...

  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...incubation can be skipped by pre-binding to 1° Monovalent Monoclonal Only anti- rabbit and -mouse versions...Maleimide-labeling occurs when a maleimide group forms a covalent bond with a sulfhydryl group (–SH), such as that...

  9. An Introduction to Adenovirus

    Type
    Blog Post
    ...likely for adenoviral vectors – namely due to the removal of certain adenoviral genes that apparently contribute...36996871. Tatsis, N., Fitzgerald, J. C., Reyes-Sandoval, A., Harris-McCoy, K. C., Hensley, S. E., Zhou...

  10. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...have been found to help improve label density (Mikhaylova, et al. 2015).  Despite these limitations, ExM.... https://doi.org/10.1038/s41587-022-01546-1  Mikhaylova M, Cloin BMC, Finan K, et al (2015) Resolving...

  11. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...problem and then explain why it’s significant and innovative. For example, "while it is possible to grow liver... best person to do this research? Do you have innovative tools? A novel dataset? Unique expertise? A novel...

  14. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...Optimization References Athey J, Alexaki A, Osipova E, Rostovtsev A, Santana-Quintero LV, Katneni U...Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org Check out Addgene'...

  15. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...

  16. Hot Plasmids - August 2020

    Type
    Blog Post
    ...derived either from p63(1-29) and Lck. Image from Chertkova et al., 2020. In their BioRxiv post, the authors...mammalian cell structure markers plasmids at Addgene! Chertkova, AO, et al., bioRxiv. 2020. https://doi.org/10.1101...

  17. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...achieve a high titer (reviewed in Joglekar and Sandoval, 2017). This high titer is one of the reasons ...https://doi.org/10.3390/v12091016 Joglekar AV, Sandoval S (2017) Pseudotyped Lentiviral Vectors: One Vector...

  18. Ras Pathway

    Type
    Collection
    ...inactive (GDP) state, regulated by the addition and removal of a phosphate group. Under normal physiological...

  19. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...inhibited. Recently, we at the Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research...a science writer at the Institute for Protein Innovation. She writes about all things IPI, from the scientists...

  20. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...are not easily reversible. Late last year, the Akhmanova Lab published a new optogenetic tool for localized...Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova, A. (2022). Opto-katanin, an optogenetic tool ...
Showing: 101 - 120 of 256 results