... process works. Equipment Autoclave Water bath Pipetman Reagents 37 g pre-mixed powder consisting of: ...10 - 15 mL of agar. Autoclavable flasks Sterile pipettes Ice bucket to hold antibiotic Antibiotic at 1000...pouring. Measure your desired amount of agar with a pipete for the first plate to get a good idea of what ...

...stab or glycerol stock . Using a sterile loop, pipette tip or toothpick, touch the bacteria growing within...plate, to create streak #2. Using a third sterile pipette tip, toothpick, or sterilized loop, drag through...

...restriction digest. Equipment Electrophoresis chamber Pipetman Pipet tips Reagents Liquid DNA aliquot of your plasmid...

...autoclave. Only mechanical pipetting should be done in the laboratory. Don’t mouth pipette! There may be some ...

... formulation buffer and sample and make sure to pipet back and forth a few times to mix the iodixanol ...buffer. Use a P1000 to the bottom of the filter and pipette up/down and wash off the walls of the filter to...

... the tube and use a sterile loop, toothpick or pipette tip to scrape some of the frozen bacteria off of...

...needed because several microliters can be lost in pipetting. For example, if you want to load 1.0 μg in 10μL...

... LB in a 2 L Erlenmeyer flask. Using a sterile pipette tip or toothpick, select a single colony from your...