We narrowed to 556 results for: SEC

...phage-assisted evolution, generating xCas9 2.0-2.6. This second round of evolution included continuous outflow ...GAT, and CAA PAMs, while xCas9 3.6 displayed the second best PAM range. Characterizing xCas9 in mammalian...efficiency fits the previous observation that NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq ...know how xCas9 is working for you in the comments section! References Hu, Johnny H., et al. "Evolved Cas9...

...genome engineering? How does CRISPR work? This section was essentially background slides getting everyone... What can CRISPR do? I spent more time on this section of the presentation because, in my opinion, it ...fewer examples and think of a way to make this section more interactive. If you can think of a good analogy...use CRISPR? How shouldn’t we use it? This last section was arguably the most important, and it was a little...

...that using Fab fragment secondaries, rather than traditional full IgG secondaries, can improve signal strength...and there’s just not much to be done about that.  Second, expansion introduces distortions. The goal in ...expansion microscopy using fluorescent Fab fragment secondary antibodies. Methodsx 9:101796. https://doi.org...

...Waldron While IHC protocols typically call for thin sections of tissue, the Wu Lab is working to create a three-dimensional...expression in the adult mouse brain using thick-section and whole-mount labeling techniques. The Trimmer...of Anti-PSD-95 [K28/43R] under the Application section of the Addgene antibody page, and learn more about... Anti-PSD-95 [K28/43R] (70 µm mounted sagittal section). Credit: Zhuhao Wu, Weill Cornell Medicine.  ...

... entirety of each day doing the experiment. The second way, it will take you 5 days to complete the experiment...advice from above, you should probably take the second option. Even if the full experiment fails, you’...need to do. In most cases, I would recommend the second option for the experiment above, but it’s also ...them. That’s why, as I’ll discuss in the planning section below, it’s important to dedicate specific amounts...

...annealing and polymerization steps could occur. Secondly, it does not work well if the ends of the fragments...fragments have stable single stranded DNA secondary structure, such as a hairpin or a stem loop (as might...vector where no other methods were available. A second example of using Gibson Assembly along with CRISPR...controlled by the presence or absence of dNTPs. Secondly, in Gibson assembly a ligase is added to repair...

... by del Valle Rodriguez et al.   LexA/lexAop A second binary system is LexA/lexAop, developed by Lai ...labs has refined the Q system and introduced the second generation QF2 activators. These activators solve...Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection of VP16, the trans-activator of herpes simplex...2843425.Takara Bio. Tet-On and Tet-Off systems: Second generation. https://www.takarabio.com/products/...

... technology has been used to optimize protein secretion and expression in the biotech workhorse yeast ...library of common parts to get you started quickly (Fonseca et al., 2019). Recently, the toolkit was used to...Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol. 6, no....Crossref, https://doi.org/10.1016/j.xplc.2020.100135. Fonseca, João Pedro, et al. “A Toolkit for Rapid Modular...

...industry, non-profit, and government sectors.  Within these sectors, you could start your own company, ...career paths in academia as well as the non-profit sector are transferrable to industry such as technology...regardless of H-1B visa number availability. In this section we will discuss a few permanent residence options...

...inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida...plasmids Selective curbing of unwanted RNA editing (SECURE) base editors Keith Joung’s lab recently found ...efficiency. Read the paper in Nature Find the SECURE base editors All-in-one AAV delivery of Nme2Cas9...

...breaking month and to find other posts that deserve a second look. 5 Great Apps for Lab Life This very useful...mention any apps you’ve found useful in the comments section to make this post even more useful to your colleagues... attention to detail when writing the post. The second post, “Running For Rare Disease, Running for FOP...

... toolkit SECURE adenine base editor Earlier this year, Keith Joung’s lab developed SECURE cytosine base...compared to rat APOBEC1. Now, they’ve engineered SECURE adenine base editor variants using a protein-truncation...

...This is the second in a 5 part series on Management for scientists. This second installment in the Management...providing feedback pretty quickly.   This is the second in a 5 part series on Introduction to Managing ...

...proceeds through the various sections, the trainee speaks first for each section, preventing the meeting from... - let us know your strategies in the comments section!   References 1. Yearly Planning Meetings: Individualized...

...education experience (co-op) is what has brought me, a second year bioengineering student, to Addgene. I am currently... completing their co-op at Addgene. This is her second contribution to the Addgene blog.      More resources... Tips for Writing a Good Cover Letter 7 Tips to Secure a STEAM Internship This Summer  ...

...collections. Addgene's "How to cite this plasmid" section Anytime you use a plasmid from the repository, ... that you can put straight into your reference section. Why do this? Not only does citing plasmids from...have come through our doors in our “Hot Plasmids” section. We’ve also added the Plasmid of the Day feature...

..., who let me know in March that I successfully secured an internship at Addgene! I was eager to accept...reached out to Amy with exciting news. I managed to secure a virtual summer program, teaching me the basics...workspaces, as well as complete the lab safety and cybersecurity training before stepping foot into the lab itself...

...primarily as protein purification tools (and can have secondary functions as detection aids.) When a single protein...performed after primary purification and requires a second clean up step to remove the cleaved tag from solution...called polyhistidine tags, typically include six consecutive His residues (or more). Histidine bonds with ...

... in and they asked me, 'How do you feel about dissecting a cat?' And I’m like, 'Well, I’m here so let’...to pour a gel [for] electrophoresis or doing a dissection video. I did that in January. This involves going...s a gel electrophoresis tray in there. Or the dissection specimens.” And since the eScience warehouse ...

...kb. ITRs are inherently unstable due to their secondary structure, palindromic nature, and high GC content...typically fails to sequence through ITRs due to their secondary structure and high GC content, but some companies...sites, and then excise the sequence between the second pair. The final result is a permanently flipped...