We narrowed to 869 results for: SOM

...kids through some fun activities. If your school doesn’t have a Math & Science Night (or some equivalent...the teachers and sharing some slides on Twitter, where I was able to get some great feedback from teachers...Absolutely not. But seeing some students really get it, and trying to answer some of their great questions...contribute to my community. This past year, I spent some time thinking about how I could combine all of these...equivalent), here are some quick tips to get things going: If your PTA has a general meeting and a means to request... if activities are run by parents who can answer some of the surprisingly technical questions elementary... who think of themselves as “scientists.” We had some accountants running our math tables!  Be realistic...

...below.  Kids and adults alike enjoyed some friendly -or in some cases, serious-  competition with our .... No, we’re not all BFFs. Yes, sometimes teams disagree. Yes, sometimes work is a bummer when you’d rather...there any have some fun together! Laura’s Top 10 Event Planning Tips! 1. Take suggestions Some of Addgene... is a higher likelihood that something will go awry. Don’t plan something that will involve a huge group...important parts of team buidling - they relieved some of the pressure of start up life and maintained ...manicured lawn of University Park at MIT.  I’m sure at some companies, employees wouldn’t be caught dead coming...us to work hard? Whatever it is, if you throw in some passion for our mission to share reagents with scientists...

...give someone a card they are more likely to remember you if you follow up and if you give someone your...is always visible. Some people learn names by seeing them. It is OK to ask someone to see their name tag... to someone and introducing yourself. Put your phone away! This is a natural time to meet someone since...up to someone much more senior than you, but you can safely seek out scientists your own age. Some will... you are on a deep level. Right away you have something in common. Namely, the scientific question you...know when you will meet a potential collaborator Some of these people you meet will review your papers... on a professional and personal level.  Here are some tips to get out of the rut of hanging out with only...

...the session, if someone comes up to my poster, I usually greet them and say something like “Let me know...Reach out if you’d like to meet someone and set aside a time to meet. Some conferences may also have a dedicated...with the policies before posting something on social media. If someone is presenting unpublished data they...include your university and your advisor’s name for some context and maybe common ground. The people you ...introduction. During the conference When you meet someone new at a conference, one of the first things you...several times at the conference, you might notice some trends. Were there parts of your elevator pitch ...arriving at the conference, plan out your agenda. Some conferences even have apps you can use to build ...

...translational control in cellular physiology. Ribosome profiling Ribosome profiling, a biochemical method developed...mRNA bound-ribosomes in a cell. When cellular mRNAs are digested with ribonucleases, ribosomes protect the... translation While ribosome profiling co-opts the physical location of the ribosome, TRICK (Translating... instead takes advantage of the ribosome’s movement. When the ribosome moves along an mRNA, it displaces...translation inhibitor that stalls ribosomes after initiation, to calculate a ribosome translocation rate of ~3.5... especially during development or stress. With ribosome profiling, researchers have been able to study...extensive sample processing to avoid disrupting ribosome-mRNA complexes. Recent work has adapted this technique...

...books for ABC. Some are easy reads, some are hard. Some I’ll never think about again, some I loved so much... or good - they're just exist and sometimes we benefit and sometimes we don't."     - Jennifer Tsang, ...most of the group really disliked, some that everyone loved, and some that ran the spectrum. I recently...Addgene. Since it’s National Book Month, we’ve asked some Addgenies to share their favorite books - both scientific...series that features a young female protagonist, some clever magic, and a satisfying conclusion? In this...age) fight in the Napoleonic Wars and against something even more sinister. 1000 pages long. Incredibly...selfish level, I wanted to be challenged to read some books outside of my comfort zone. I’d been in a ...

...delivered and expressed in multiple somatic cells via various methods. Some of the more common delivery methods... epithelial cells from urine are some of the easily isolated somatic cells that have been reprogrammed...discuss the two major processes by which an adult somatic cell is converted to a different cell type for ...conversion happens via: ReprogrammingA differentiated somatic cell reverts back to a pluripotent state, which...Transdifferentiation In this process, differentiated adult somatic cells are converted to cells of a different lineage...stages: Dedifferentiation - Conversion of adult somatic cells into the pluripotent state. Redifferentiation...KLF4, and C-MYC - that induce a differentiated somatic cell to revert back to a pluripotent stage (iPSC...

...cytoplasmic contents. Autophagosomes that have fused with the vacuole or a lysosome are trickier to identify...localization or accumulation of pre-autophagosomal structures, autophagosomes and autophagic bodies in yeast...vacuole or lysosome, since both Atg8p and LC3 are degraded or released once an autophagosome fuses with... targets cytosol and organelles to the vacuole/lysosome for degradation and recycling. The key to Rosella...radio-labeled short-lived proteins primarily via proteasome degradation. Autophagy is then induced and quantified... autophagic structures based on morphology. Autophagosomes are relatively easy to identify: double-membraned...Atg8p (“p” stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for...

...the voice-over in videos), I sent some directions and (rules) to some volunteers, who filmed with their...setup to run some remote interviews, so the interviewee could see the interviewer. We’ve got some really cool...projects lined up, some that started production during the middle of lockdown, and some that will spin up... videos for free. We’re a small operation, so sometimes it can be a big challenge. But ask any filmmaker...It’s really nice to have a scientist on-screen, somebody who really knows the protocol and can guide viewers...viewers through. I know cameras, but we needed someone else. Design: Jennifer thankfully volunteered, ...for longer than anticipated, we had to think of something else.   Figure 2: Ironing boards. Good ...

... However, some bicistronic vectors take advantage of an element called an Internal Ribosome Entry Site...eukaryotic cells, and, although some scientists report close to 100% cleavage with some of these peptides, no consensus...the genes expressed from the same cassette is sometimes advantageous, particularly when only a portion...multicistronic vectors work? Scientists have "borrowed" some tricks discovered in positive single-stranded RNA... can see that the IRES element acts as another ribosome recruitment site, thereby resulting in co-expression...have been discovered - many from viruses, but also some from cellular mRNAs. What they all have in common...found in bicistronic vectors; however, they do have some disadvantages. These elements are quite large (500...

...but some purification kits make it easier than others to prevent quality issues. For example, some kits...one you are most familiar with. And, hey — if something is working for you, then there is no reason to...an old standby to fall back to? We have outlined some important considerations to help you choose the ...need to recover and the level of quality required. Some applications require very little DNA and you can...source of waste in laboratories. To help combat this, some manufacturers have designed eco-friendly DNA purification...bottles or recycled cardboard packaging, there are some less obvious improvements that reduce the environmental...Separately Yes, buffers and columns sold separately Some, waste tubes sold separately No Other ...

...different. Some of these changes you may enjoy immediately and some of them may just take some getting used... counterpoints to some of the causes of culture shock above, but after polling some Addgene colleagues...settings: The sense of being the first to discover something. The thrill of having an experiment yield clear...if I was asked that question today, because I sometimes wish for a stretch of time that doesn’t involve...mentally challenging than lab work at times.” Sometimes a desk job is defined as sitting down at a computer... between tubes, look through microscopes, and sometimes you even wear a lab coat! These are the things...is that I’ve never been bored. There is always something to do and there is no thumb twiddling. But you...

...1952 was noted to integrate into the E. coli chromosome in some cases. This terminology held until the 1960s...-factors could integrate into the chromosome. Thus the term “episome” was eventually dropped and we’ve...Latin for 'it'), as “a generic term for any extrachromsomal hereditary determinant”. His proposal, however...however, was basically ignored. A separate term, “episome”, defined as “a non-essential genetic element which...exist either autonomously or integrated into the chromosome” was proposed a few years later by Élie Jacob...name for these elements. At the time, the use of episome seemed appropriate, especially since the Fertility...1960s when scientists began to study other extrachromosomal particles, particularly Resistance or R-factors...

... can see some of Joanne’s comments on the AMA process below, but we also wanted to share some thoughts..., but for many of us, scientist is something we are, not something we do. We have to find a way to keep...decided to do an AMA in the first place and give you some reasons why you should consider using Reddit to ...try to keep discussions on-topic and, at least sometimes, civil. r/Science in particular hosts a variety... topics as our first foray into Reddit. Here are some reasons why: 1. Reddit has a large and active community...community of people interested in science. You can see some of the conversations going on within the community...Management Software for Biology) on Reddit and got some substantial traffic that we thought it would be ...

... (FISH) and chromosome capture suffer from low resolution and can’t be used in vivo. Some researchers ...two different pairs of chromosomes using gRNAs specific to sequences on chromosomes 9 and 13. They next ...CRISPRainbow was used to track the localization of 6 chromosome-specific loci in the U20S cancer cell line./ ... color represents a gRNA targeting a specific chromosome. This image represents the composite of fluorescent...puncta observed. By labeling two genes of a given chromosome simultaneously, they could also monitor the spatial...distances from 2-75 Mbp. Labeling multiple, intrachromosomal loci Building on the work of Chen et al., ...turned their attention to mapping pairs of intrachromosomal loci. The technique successfully resolved ...

...vesicles to more acidic lysosomes. Though a number of biosensors existed that met some of the criteria necessary...physiology, TRIM21 recruits ubiquitin and the proteasome to destroy antibody-bound pathogens. Clift et...rapid, targeted degradation that is dependent on proteasome function. TRIM-away compared favorably to drug...overexpression does not aberrantly activate the proteasome. Clift et al. tested multiple methods for TRIM-Away...to the culture plate within 4 hr of treatment. In some primary cell lines, endogenous TRIM21 is sufficient... et al. Cell. 2017. PubMed PMID: 29153837. Endosomal pH sensors for STED nanoscopy Article contributed...contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment The first Addgene plasmid deposit...

...procedure can and will fail. Some step failures require re-attempting the same step, some require going back two...In our second example we’ve added some simple but critical logic. Some steps fail “hard”, they reset the...choice than inheritance. Some containers are permanent, like a tray or a freezer. Some are disposable, like...through the cracks. In this post we’ll highlight some of the lessons we’ve learned over the years with... two steps, some require starting over entirely, and our software needs to handle these cases. The following...example to the right (# this will make you sad),  someone has put in a request for a particular plasmid and...are no explicit instructions on what to do when something fails. While we’re sure our techs could figure...

...Importantly, the panel offered some great solutions to these problems and some that stuck out to me were: ...providing means to answer some of these questions and provided ways to solve some of the structural problems...enterprise going forward, in this post I’ll point out some of the highlights and takeaways I got from the symposium... many graduate students/post-docs?” You can find some of the more detailed questions the organizers wanted...nationality, gender, race, etc), or their career outcomes. Some of the more specific contours of what we need to...fantastic job of articulating many of these problems and some solutions. The structural problems seem to boil ... publishing in the current academic environment. Some great tips from this panel included:  Write Often...

...becomes an overnight success. Below I have detailed some tips to improve your grant writing and hopefully...research and write your application, then give it to someone else to read and / or give yourself a bit of a ...mentor (your supervisor/ a previous supervisor / someone else in the department) to read over your application...these findings will be worth knowing? This can sometimes be tricky, as most of our research builds on ideas...in it?) so make sure that comes across. Check out some in-depth grant writing tips at the Human Frontier...conversing with other scientists that we forget that some of the terminology we use routinely isn’t common...commercial organizations, or universities may have someone senior from accounts or HR on the judging panel...

...identified by various enzymes and sent to the proteasome for degradation. The tags we will discuss here...can yield intermediate protein abundance. Finally, some degron tags can do more than just degrade! Read ...will review the 4 most common types of degrons and some of the nuances to each one.   SMASh Small molecule-assisted...cleavage sequence. The tag is then shuttled to the proteasome due to the presence of the degron, and the protein...domain shuttles the entire tagged protein to the proteasome for degradation. In addition to having the coolest...protein is not amenable to being tagged, e.g. tagging some proteins can affect their function, the SMASh tag...system also requires less genetic engineering than some of the other systems reviewed here. The only required...