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In Category

We narrowed to 1,036 results for: STI

Showing: 101 - 120 of 1036 results

  1. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...desired restriction sites and add them to your existing vector. It is a couple days of work that will ...the overhangs generated by restriction digest of existing sites in the original vector. Designing oligos...bases to complement the overhangs generated when digesting the vector with EcoRI and SalI (see diagram )....

  2. Water Bath Protocol

    Type
    Protocol
    ...Equipment Water Bath 10% bleach or 70% ethanol Distilled water Disinfectant Thermometer Water bath weights...the edge of the slide. Fill the water bath with distilled water, not tap water, to prevent salts from accumulating...water in it from previous uses. However, you will still need to regularly clean out the water bath even ...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid Collection FRET Biosensors...-pmCherryC1 Actin Filaments Cortactin mCherry Christien Merrifield 36201 pLifeAct-mTurquoise2 Actin Filaments...Protein Collection . Return to top Do you have suggestions for other plasmids that should be added to this...

  4. Fluorescence Titering Assay

    Type
    Protocol
    ...percentage of fluorescent cells, these cells can still be used in downstream applications. Safety Warnings...ensure that you are in compliance with your institution’s biosafety regulations. Workflow Timeline Day...with multiple integration events leading to underestimation of the true titer. Calculate the transduction...

  5. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...partnership to provide AAV viral preps, includes listing of transfer status for viral preps....Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes may be available) Based...Wilson Please contact [email protected] with any questions. Return to top...

  6. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...genome-containing virions. Phenol red is added to clearly distinguish the steps. This method will help enrich the prep...ultracentrifugation. The steps are clearly distinguishable thanks to the use of phenol red. Image adapted...cartoon indicating the position of the needle for harvesting of the purified AAV using option #1. Right image...

  7. Neuroscience

    Type
    Collection
    ...Reactivity Recommended Applications PI Do you have suggestions for other antibodies that should be added to ... represent the official views of the National Institutes of Health....

  8. Depositor Collections

    Type
    Collection
    ...- Davidson Allen Institute for Brain Science AAV Enhancer Collection Allen Institute for Cell Science ...

  9. Pouring LB Agar Plates

    Type
    Protocol
    ... bath Pipetman Reagents 37 g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein...mL of agar and on which you can individually distinguish a maximum of ~100 colonies Pro-Tip If you will...After overnight drying, we place the plates in a plastic bag with an absorbent material to reduce condensation...

  10. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...mammalian cells or zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One...mammalian cells or zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One...

  11. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...please let us know using the Cloning Grade DNA Suggestion Form . Please note that this does not guarantee...us of which plasmids would be most helpful to requesting scientists. Return to top...

  12. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...phytochrome photoreceptors (BphPs). Spectrally distinct permanently fluorescent iRFP variants have been...photoactivation) 3.0 pPAiRFP2-N1 Do you have suggestions for other plasmids that should be added to this...

  13. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...on how it was originally used. Here are a few questions to consider when choosing a gRNA: Does a given...Insert Vector Type PI Publication Do you have suggestions for other plasmids that should be added to this...

  14. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... and Cascade on one single plasmid, though many still separate the individual Cas components. Figure 1...Last reviewed on: January 30, 2025 Do you have suggestions for other plasmids that should be added to this...

  15. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing...cloning to insert the desired guide RNA sequence by digestion of the backbone with BbsI followed by a quick ...

  16. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs and tagging of genes of...Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs in yeast...

  17. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...viral vector production is a common step after requesting plasmids from Addgene, and by providing this ...storage. Biosafety Guide Documentation for your institutional biosafety office and your lab, information on...

  18. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...lentiCas9-Blast 1 and then selected with 1 μg/mL blasticidin for 9 days. Single cells were then plated in ...transduced (MOI = 37) and selected with 1 μg/mL blasticidin for 9 days. Single cells from a stable cell pool...then expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed...

  19. CRISPR Plasmids - C. elegans

    Type
    Collection
    ... BbsI, BsaI Ingestion by C. elegans none, need Cas9 plasmid Ristow Do you have suggestions for other plasmids...

  20. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... weeks after the approval of the MTA by your institution. Our final step of the QC process, viral genome...communicated by email will supersede the general estimated turnaround time above. After starting viral production...
Showing: 101 - 120 of 1036 results