We narrowed to 172 results for: TOX;

1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   ...As nucleases may have off-target effects and/or toxicity, it would be advantageous to instead use a preexisting...

2. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   ...desired product while avoiding the accumulation of toxic intermediates or the shuttling of the intermediates...

3. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   ...labeling approach, is an alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein...

4. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   ...results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing...

5. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   ...substitutions are slightly more efficient and less toxic to mammalian cells (Allen, et al., Frontiers in ...

6. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   ...Slaymaker et al. showed that these mutants are not toxic to HEK cells and that similar mutations can improve...

7. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   ... using the rTetR system, but Cre-DOG lacks the toxicity that can be seen with high levels of transcription...

8. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   ...stop it from being used. One thing is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow ...

9. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

   Type

   Blog Post

   ... the plasmid will produce anything dangerous or toxic to humans in bacteria, select “yes” in the drop-...

10. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    ... long-term transgene expression and negligible toxicity. However, their production is more complex and...

11. Rabies and Neuronal Tracing

    Type

    Blog Post

    ...First, these high levels of viral replication are toxic. Second, infecting subsequently higher and higher...

12. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    ...added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use it under a ...

13. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    ... efficiency in cells that are sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells...

14. Bacterial Expression Systems

    Type

    Collection

    ...Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and visualization inside living...

15. CRISPR Pooled gRNA Libraries

    Type

    Collection

    ...2 plasmid) Knockout Human Moffat 3rd 4 70,948 Toxoplasma Knockout 80636 Knockout T. gondii Lourido N/A...

16. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    ...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...

17. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    ... for a single insert), mostly due to the use of toxic ccdB to prevent any non-recombinant vectors from...

18. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    ...acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split very soon. There...

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    ...genome, removing the risk of oncogenicity or genotoxicity seen with other vectors (Lukashev et al., 2016...

20. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    ...performed 12–18 hours post-transfection to help remove toxic transfection reagents and keep the cells happy. ...