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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 888 results for: TRI

Showing: 101 - 120 of 888 results

  1. Neurodegeneration Research Collection

    Type
    Collection
    ...caused by abnormal expansion of a repeating CAG triplet series within the huntingtin (HTT) gene. An individual... the disease, a small portion of cases can be attributed to known genetic factors such as defects in LRRK2...

  2. CRISPR References and Information

    Type
    Collection
    ...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...Goldstein Nematode: Injection and selection for Cas9-triggered homologous recombination Cas9-sgRNA target construct...

  3. Optogenetics AAV Preps

    Type
    Collection
    ... is typically used in neuroscience to control electrical potentials in neurons. See our Optogenetics Plasmids...pAAV-Ef1a-fDIO-ChrimsonR-tdTomato EF1a ChrimsonR tdTomato Flp dependent 1, 9 Patricia Jensen 174007 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST...

  5. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...was first described in 1966 by Austrian physician, Andreas Rett, who tried to raise awareness and published...binds to methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson & Bird...in which too much of the MECP2 protein is as detrimental as too little, resulting in MECP2 duplication...advance their respective gene therapies to clinical trials in 2021. Figure 1: Schematic of the human...

  6. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...for most purposes, but what do you do when you’ve tried a common strain and don’t get the desired level ...inactivated native restriction/methylation system. This means the strain can neither restrict nor methylate...expression of T7 RNAP from the genome; does not restrict methylated DNA fhuA2 lacZ::T7 gene1 [lon] ompT...adding the inducer. Growth Media: Changing media is tricky, because there can be a trade-off between growth...about your favorite protein expression tips and tricks! Leave a comment below or browse Addgene’s collection...

  7. Plan Your Experiment

    Type
    Collection
    ... containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning ...Some prime and base edits Can be paired with restriction enzymes for further customization Usually paired...

  8. Biosensor AAV Preps

    Type
    Collection
    ...Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Patriarchi Oxytocin (OT...

  9. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Vector Core produced viral-based vectors for distribution via Addgene. For a full list of Addgene's viral... The following vectors are now available for distribution at Addgene: Penn ID Addgene ID Name Function...

  10. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ...Collection is a quick guide to a smaller set of tried-and-true winners, containing enhancers with the ...Enhancer Collection!"{% endraw %}{% end_module_attribute %}{% module_attribute "child_css" is_json="true" %}...%}{% raw %}{}{% endraw %}{% end_module_attribute %}{% module_attribute "css" is_json="true" %}{% raw %...padding-right":"172px"}{% endraw %}{% end_module_attribute %}{% module_attribute "definition_id" is_json="true...%}{% raw %}null{% endraw %}{% end_module_attribute %}{% module_attribute "field_types" is_json="true" ...style":"group"}{% endraw %}{% end_module_attribute %}{% module_attribute "label" is_json="true" %}{% raw... raw %}null{% endraw %}{% end_module_attribute %}{% module_attribute "link" is_json="true" %}{% raw %}...

  12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...flow strips that contain streptavidin which can bind to the biotinylated targets. They also tried to confirm...lateral flow strips. Since the probes are tagged with streptavidin, the lateral flow strip first captures...This post was contributed by Shravanti Suresh from Iowa State University. Since its appearance, SARS-... this virus has become an utmost priority for countries around the world and to do so, the WHO recommends...testing, tracking, and social distancing. With countries like South Korea, one of the earlier epicenters...be captured by an antibody further along on the strip. Highlights: Of the 83 known COVID-positive samples...viruses. Unlike Cas12a, Cas13a does not require strict sequence preferences at the target site, while ...

  13. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...’s catalog — from the recently developed to the tried-and-true classics. Check out our Biosensors Collection...biosensors new to Addgene this year. Feel the love with MTRIA sensors One of the latest additions to Addgene’s...TRansmembrane region-to-IntrAcellular loop linkers” (MTRIA for short), where a fluorescent protein is inserted... ligand (Ino et al. 2022). The oxytocin sensor MTRIAOT enabled real-time observations of oxytocin dynamics...earlier tools. They also produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters...ligands - almost A to Z! - from ATP to urotensin. The MTRIA sensors will be useful for investigating neuronal...sensors for these and other targets. Figure 1: MTRIA biosensors are generated by inserting a circularly-permuted...

  14. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...get them below a given threshold even when you’ve tried everything listed here. For some applications, this...CRISPR to (hopefully) be used in human clinical trials. An off-target rate of 5% is far too high when ...

  15. Zinc Finger Consortium Reagents

    Type
    Collection
    ...engineering and expressing zinc finger proteins for distribution to academic and nonprofit laboratories for research...

  17. Antibodies

    Type
    Collection
    ... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate...

  18. KLF Research Plasmids

    Type
    Collection
    ... plasmids containing KLF, we encourage you to contribute to this community resource. Learn about the benefits...

  19. Depositor Collections

    Type
    Collection
    ...Collections Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection Disease ...
Showing: 101 - 120 of 888 results