We narrowed to 125 results for: actin

1. Your Lentiviral Plasmid FAQs Answered

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   ... follows: Transfer vectors contain minimal cis-acting HIV components: LTRs, PPT, RRE, and psi packaging...

2. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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   ...can check colonies either by PCR, by directly extracting and sequencing the plasmid, or both! We strongly...

3. Hot Plasmids Spring 2024

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   ...CRISPRi screen to identify additional host factors impacting prime editing and found the protein La (a small...

4. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ...7 mutations found in the REC2, REC3, and PAM interacting domains and allows for expanded PAM recognition...

5. An Inside Look into Addgene's Dev Team

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   ...sharing knowledge allows for life to happen without impacting the overall work. Each of our sprints is themed...

6. Transferable Skills Guide: Career Planning Resources

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   ...information, but not a job! This is good etiquette when contacting someone professionally that you do not know very...

7. Career Insights: Technical Support Specialist

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   ...and more. Technical support roles also involve interacting with other departments such as research and manufacturing...

8. Supporting Reproducibility with a Connected ELN

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   ...one foot on the other side of the pond through interacting with colleagues in Edinburgh. You can follow ...

9. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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   ...fusion and a modified gRNA that is capable of interacting with an additional RNA-binding transcriptional...

10. Tips for Getting a Faculty Position

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    ...focus on you and your ideas. Slides will be     distracting. What is the big question? This should be...

11. Plasmids 101: Expressing shRNAs for RNA Interference

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    ...RNA (shRNA) Short interfering RNA (siRNA) Piwi-interacting RNA (piRNA) miRNA and piRNA can be found endogenously...

12. Transferable Skills Guide: Time Management

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    ...internet and all it’s enticements are far too distracting. (As a side note, I’d recommend the book, “Bored...

13. A Control for All Seasons

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    ...secondary antibody, then that secondary antibody is reacting non-specifically to one of the other primary antibodies...

14. Is this the right place for me? 8 tactics for choosing a lab

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    ... also be a sign that the lab head has trouble attracting scientists. To get a better idea of why the lab...

15. A History of Genome Engineering in Popular Culture

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    ...storyline involves the resurrection of dinosaurs by extracting the DNA from fossilized amber and supplementing...

16. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    ...analysed by mass spectrometry to identify the interacting proteins. Similarly, in ChIP-seq assays, chromatin...

17. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    ...emission of labeled agonists and antagonists, impacting the interpretation of experimental results. It's...

18. How to Deposit Your Plasmids with Addgene

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    ...greater than 75 plasmids, please let us know by contacting [email protected]. These deposits are considered...

19. A Needle in a Base-Stack: Cas9 Structural Biology

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    ...DNA, the CTD is also sometimes called the PAM-interacting (PI) domain. The guide RNA is bound primarily...

20. Antibody Validation for Flow Cytometry

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    ...the intracellular compartment may contain cross-reacting antigens not present on the cell surface. The ...