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Showing: 101 - 120 of 1033 results

  1. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

  2. Microbiology Resources

    Type
    Collection
    ...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

  3. AAV Packaged on Request

    Type
    Collection
    ...implementation letter DNA amplification Viral vector production Density centrifugation purification Addgene’s comprehensive...preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures such...order ships. Shipping time will depend on your location. Share Your Results Once you start getting results...high-quality viral vector preps at Addgene, including purification, titration, and quality control measures. We...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ... Return to Top Other Tet Applications Explore some other popular applications of tetracycline systems ...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated... Tet-On 3G transactivator Tet-On 3G rtTA Elena Cattaneo 104543 PB-EF1a-TetOn3G PiggyBac vector expressing...-tag from TRE3G promoter for Tandem-Affinity Purification. Yannick Doyon 20321 TetO-FUW-OSKM Tet-On inducible...

  5. Genetic Code Expansion

    Type
    Collection
    ...a ncAA into a protein of interest, four key modifications to the standard translation machinery are required...with a different amino acid. To achieve these modifications, two plasmids are generally used. Once these...amino acids (ncAA) in a protein of interest. Applications By making small changes in selected amino acids...code expansion without buildup of prematurely truncated protein. Ryan Mehl 197656 BL21(DE3) ΔserC serC...gene replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses archaeal MjTyrRS...genes replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses archaeal MjTyrRS...Davis L. & Chin J. W. (2012). Designer proteins: applications of genetic code expansion in cell biology . ...

  6. Plant Plasmids and Resources

    Type
    Collection
    ...contains yeast two-hybrid and bacterium-to-plant translocation vectors. Partially compatible with GoldenBraid...multi-purpose toolkit that enables targeted, specific modification of monocot and dicot genomes using TALENs and...Successfully validated in tomato, tobacco, Medicago truncatula , wheat, and barley. Return to top Gene Silencing...organelles, membranes and cytoskeletal elements in M. truncatula . Return to top Other Plant Resources Addgene...Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature...

  7. TALEN Plasmids and Kits

    Type
    Collection
    ...Golden Gate TALEN kit. The GoldyTALEN scaffold is truncated at both the N and C terminus and induces mutation...pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed...in vitro mRNA synthesis from the T7 promoter. Truncations were introduced to the N- and C-terminus of the...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...expression in mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller...

  8. Rett Syndrome

    Type
    Collection
    ... disruptions, missense mutations located in the MBD, and truncations prior to the NID are more severe ...even within patients with the same mutation, the location and type of MECP2 mutation can be a strong predictor...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...DNA) Mutation (protein) Sex Source of Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link...

  9. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top ...top Plant ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

  10. Cancer Research Plasmids and Resources

    Type
    Collection
    ...defined by their location, their pathway dependencies are extremely important for classification and treatment...network for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential ... described in the initial Target Accelerator publications, focused on somatic variants found in lung adenocarcinoma...

  11. Luciferase Plasmid Collection

    Type
    Collection
    ...bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission...advantages and disadvantages depending on the application, and different luciferases require different ... course experiments, but is too dim for some applications. NanoLuc® is small (19 kDa), very bright, and... available, making it ideal for a variety of applications. To learn more and plan your experiment, read...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored version...

  12. Retrograde AAV viral preps

    Type
    Collection
    ...the functional catagories listed below. Narrow down the items available within a category by using the ...experiments can be found on the material pages for viral catalog items. Browse Available Retrograde AAV See our ... Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  13. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...cited in publications. All pricing includes DNA amplification, viral vector production, purification, and ...Look up the IDs of particular Penn Vector Core catalog items. Virus-associated DNA Each viral vector service...

  14. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...top Plant ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

  15. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its usefulness...developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh promoter with EF1alpha...Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B., Park, K. U.,... alters photoreceptor and bipolar cell fate specification, yet differs from deleting both genes . Development...

  16. AAV for Neuronal Tracing

    Type
    Collection
    ...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  17. Viral Vector Guides and Plasmids

    Type
    Collection
    ...strong immunogenic response, useful for clinical applications as vaccines. Selecting a Viral Vector Type Each...used to transduce cells of interest. Specific modifications to these plasmids, such as different promoters...been carefully engineered for safety and are replication-incompetent. Specific genes have been mutated...mutated or deleted to prevent uncontrolled viral replication, and the required genes are divided in the multi-plasmid...

  18. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Histones 109119 CTNNB1-mEGFP AICSDP-47 mEGFP Beta-catenin Adherens junctions 107579 RAB5A-mEGFP AICSDP-39...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...the Allen Institute for Cell Science Cell Catalog (Link opens in a new window) . Protocols The Allen Institute...: 28814507 (Link opens in a new window) More publications from the Allen Institute for Cell Science (Link...

  19. Promega Plasmid Collection

    Type
    Collection
    ...purpose-driven nonprofit, Addgene offers expert support, educational resources, and efficient material transfer solutions...Promega technologies that support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT...useful for both intracellular and extracellular applications where you want minimal disruption of endogenous...developed for special functions that vary from purification, visualization, and functional studies. You ...

  20. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...copGFP from a truncated EF1 promoter pCDH-EF1S-Nluc 73033 Expresses Nluc from a truncated EF1 promoter ...pCDH-EF1s 72484 Express gene of interest from a truncated EF-1 promoter pCDH-EF1-Luc2-P2A-copGFP 72485 Expresses...pCDH-EF1s-copGFP 73034 Expressed copGFP from a truncated EF1 promoter pCDH-CMV-Nluc-P2A-copGFp-T2A-Puro...
Showing: 101 - 120 of 1033 results