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We narrowed to 306 results for: construct

Showing: 101 - 120 of 306 results
  1. Hot Plasmids - February 2022

    Type
    Blog Post
    ... Figure 1: (a) Anti-FLAG frankenbody (FB) construction from the complementarity determining region ...and HA tags, you may not even need to clone new constructs to label your favorite protein! While the target...
  2. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...experiments. Manufacturers rarely provide the construction plans or CAD (computer aided design) drawings... that allow for on-going creativity with the construction plans. Appropriate open-source licenses for ...
  3. Plasmids 101: Cre-lox

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    Blog Post
    ...Cre-lox technology, transgenic mice have been constructed that express Cre under a variety of ubiquitous... many loxP-containing alleles have also been constructed. Additionally, Cre-containing adenovirus (Ad-...
  4. Neurodegeneration Research Collection

    Type
    Collection
    ...structure and function with siRNA-resistance SigmaR1 constructs . (Sawyer et al. Dev Cell. 2024) Image exocytosis...Initiative Collection . Use the 3xFlag-eGFP-Flag-SETX construct for subcellular localization studies with GFP,...Cell. 2017) Study amyloid deposition with APOE2 constructs . (Hudry et al. Sci Transl Med. 2013) Express... neurons with inducible lentiviral expression constructs: pLVX-UbC-rtTA-Ngn2:2A:EGFP (Schafer et al. Nat...
  5. Hot Plasmids: Spring 2025

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    Blog Post
    ...transposon RNA homology both help direct the fusion construct to the new genomic locus. Image courtesy of Christopher...2025).More than a dozen of these enhancer-AAV constructs are now available as in-stock viral preps using... Look for more of these interneuron-targeting constructs and other serotypes available later this year...
  6. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    Blog Post
    ...file. What's available at Addgene? In total, we constructed and tested eight two-input RSMs and three three-input...three-input RSMs. The plasmids used to construct these RSMs are listed in the table below and can be requested...S10A pNR64 pNR166 Fig. S10B pNR64 pNR187 To construct your own two-input or three-input GRSMs, we recommend...
  7. Brzezinski Lab CRISPR Collection

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    Collection
    ...stranded break constructs. Plasmids 171098 and 175570 allow conversion to a dual guide construct. Plasmids ...
  8. TALEN Guide

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    Collection
    ...small, repeat-laden DNA regions into a single construct would be technically challenging. Thanks to efforts... of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian...6051):1843-6. Pubmed PMID: 21960622 . Efficient construction of sequence-specific TAL effectors for modulating...
  9. Important Considerations When Using AAVs

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    Blog Post
    ...AAV vectors to deliver chemogenetic/optogenetic constructs into our cells of interest. Although they have...time until expression, it is possible to use AAV constructs with self-complementing structure (scAAV) (7)... would result in cell death. With several new constructs becoming available, AAVs could be much better...
  10. Malate Dehydrogenase CUREs Community Collection

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    Collection
    ...assay, and easy to purify using histidine-tagged constructs and routine protein and molecular biology techniques... window) Acknowledgments The MCC and plasmid construction was funded by NSF IUSE 1726932 and NSF RCN-UBE...
  11. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...interest. These plasmids will be most useful for constructing intermolecular FRET probes, where the donor ...may be able to boost FRET efficiency. Before constructing your own FRET probe, try searching PubMed for...
  12. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...library delivers 2 randomly paired guides per construct and allows a reduced scale in screens without ...serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1...
  13. Hot Plasmids - October 2020

    Type
    Blog Post
    ...Figure 1:Schematic diagram of different FingR constructs and experimental results. Image from Bensussen...Bensussen et al., 2020. The FingR constructs target the synaptic scaffolding proteins PSD95 and gephyrin...
  14. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post
    ...have deposited the best performing sensors and constructs encoding corresponding portions of the Zika virus...virus research community. Find the Zika Sensor Constructs at Addgene As we report, we have embedded this...
  15. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...the plasmid name: This is the name given to the construct by the depositing lab. There may be other plasmids... Principal Investigator (PI) in whose lab the construct was made. The depositor name links to a page listing...
Showing: 101 - 120 of 306 results