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We narrowed to 310 results for: construct

Showing: 101 - 120 of 310 results

  1. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...experimental work, spend some time outlining the construction of the plasmid and all the steps you will have...used for cloning of more than 10,000 DNA custom constructs. His work was acknowledged in hundreds of publications...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...Cre-lox technology, transgenic mice have been constructed that express Cre under a variety of ubiquitous... many loxP-containing alleles have also been constructed. Additionally, Cre-containing adenovirus (Ad-...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...transposon RNA homology both help direct the fusion construct to the new genomic locus. Image courtesy of Christopher...2025).More than a dozen of these enhancer-AAV constructs are now available as in-stock viral preps using... Look for more of these interneuron-targeting constructs and other serotypes available later this year...

  4. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...file. What's available at Addgene? In total, we constructed and tested eight two-input RSMs and three three-input...three-input RSMs. The plasmids used to construct these RSMs are listed in the table below and can be requested...S10A pNR64 pNR166 Fig. S10B pNR64 pNR187 To construct your own two-input or three-input GRSMs, we recommend...

  6. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems. ...and Genomic Tools Reporters and Tags Library Construction Integration Recombination Shuttle Vectors Plasmids...

  7. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... can be used to turn shRNA constructs on or off. In floxed-shRNA constructs, Cre can excise the shRNA ...lox-STOP-lox shRNA constructs, Cre expression promotes shRNA expression. Gene Switch: These constructs contain two...regulate your gene of interest. Depending on the construct, gene expression may be activated or repressed...

  8. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...of each CRISPR/Cas9 construct (10 μg total). Add 0.5 μg of GFP expression construct. Electroporate cells...does not contain markers for selection 1 . Other constructs may be utilized, such as pX458 (Addgene plasmid...puromycin as selectable markers, respectively, or constructs in which multiple sgRNAs may be expressed from...yield. Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE...conditions for each cell line with a reporter construct to ensure robust plasmid delivery before attempting... that received high levels of the CRISPR/Cas9 constructs. Plate sorted cells individually into 96-well...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    ...AAV vectors to deliver chemogenetic/optogenetic constructs into our cells of interest. Although they have...time until expression, it is possible to use AAV constructs with self-complementing structure (scAAV) (7)... would result in cell death. With several new constructs becoming available, AAVs could be much better...

  10. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...interest. These plasmids will be most useful for constructing intermolecular FRET probes, where the donor ...may be able to boost FRET efficiency. Before constructing your own FRET probe, try searching PubMed for...

  11. Hot Plasmids - October 2020

    Type
    Blog Post
    ...Figure 1:Schematic diagram of different FingR constructs and experimental results. Image from Bensussen...Bensussen et al., 2020. The FingR constructs target the synaptic scaffolding proteins PSD95 and gephyrin...

  12. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...library delivers 2 randomly paired guides per construct and allows a reduced scale in screens without ...serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1...

  13. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...have deposited the best performing sensors and constructs encoding corresponding portions of the Zika virus...virus research community. Find the Zika Sensor Constructs at Addgene As we report, we have embedded this...

  14. Cancer Research Plasmids and Resources

    Type
    Collection
    ...including mutant, knockdown, and overexpression constructs. Each collection page listed below organizes ...Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene expression vectors for in...

  17. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...the plasmid name: This is the name given to the construct by the depositing lab. There may be other plasmids... Principal Investigator (PI) in whose lab the construct was made. The depositor name links to a page listing...

  18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription Reporter:...at Addgene. Cell marking/selection: Expression constructs like plasmids often include GFP as a marker to...

  19. Plant Plasmids and Resources

    Type
    Collection
    ...vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre Marillonnet and Johannes Stuttmann...containing several cassettes and generate multi-construct transgenic plants - Jan Lohmann. MoClo Plant Parts... for hierarchical assembly of plant multigene constructs - Johannes Stuttmann. MoChlo: Modular Cloning...

  20. TALEN Guide

    Type
    Collection
    ...small, repeat-laden DNA regions into a single construct would be technically challenging. Thanks to efforts... of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian...6051):1843-6. Pubmed PMID: 21960622 . Efficient construction of sequence-specific TAL effectors for modulating...
Showing: 101 - 120 of 310 results