...transposon RNA homology both help direct the fusion construct to the new genomic locus. Image courtesy of Christopher...2025).More than a dozen of these enhancer-AAV constructs are now available as in-stock viral preps using... Look for more of these interneuron-targeting constructs and other serotypes available later this year...

...file. What's available at Addgene? In total, we constructed and tested eight two-input RSMs and three three-input...three-input RSMs. The plasmids used to construct these RSMs are listed in the table below and can be requested...S10A pNR64 pNR166 Fig. S10B pNR64 pNR187 To construct your own two-input or three-input GRSMs, we recommend...

...AID* tagged CRISPR knock-in repair constructs. These repair constructs can be used to integrate the tagged...

...Worm Plasmids and Resources Plasmid Tools Worm Constructs Kits and Collections Other Resources The nematode...characterized which allow researchers to express desired constructs in precise cell types and/or developmental timeframes...plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples of useful...elegans . Synthetic Biology Synthetic biology constructs - Browse Addgene's collection of synthetic biology...

...AAV vectors to deliver chemogenetic/optogenetic constructs into our cells of interest. Although they have...time until expression, it is possible to use AAV constructs with self-complementing structure (scAAV) (7)... would result in cell death. With several new constructs becoming available, AAVs could be much better...

...Figure 1:Schematic diagram of different FingR constructs and experimental results. Image from Bensussen...Bensussen et al., 2020. The FingR constructs target the synaptic scaffolding proteins PSD95 and gephyrin...

...interest. These plasmids will be most useful for constructing intermolecular FRET probes, where the donor ...may be able to boost FRET efficiency. Before constructing your own FRET probe, try searching PubMed for...

...library delivers 2 randomly paired guides per construct and allows a reduced scale in screens without ...serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1...

...have deposited the best performing sensors and constructs encoding corresponding portions of the Zika virus...virus research community. Find the Zika Sensor Constructs at Addgene As we report, we have embedded this...

... free distribution services for their popular constructs, why not let us know? Fill out our referral form...

...related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems. ...and Genomic Tools Reporters and Tags Library Construction Integration Recombination Shuttle Vectors Plasmids...

...activator. While it requires a relatively simple construct, it exhibits modest levels of gene activation,...than SAM. One drawback of this method is its construction: it relies on antibody chains, which are relatively...

...the plasmid name: This is the name given to the construct by the depositing lab. There may be other plasmids... Principal Investigator (PI) in whose lab the construct was made. The depositor name links to a page listing...

... Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription Reporter:...at Addgene. Cell marking/selection: Expression constructs like plasmids often include GFP as a marker to...

...of each CRISPR/Cas9 construct (10 μg total). Add 0.5 μg of GFP expression construct. Electroporate cells...does not contain markers for selection 1 . Other constructs may be utilized, such as pX458 (Addgene plasmid...puromycin as selectable markers, respectively, or constructs in which multiple sgRNAs may be expressed from...yield. Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE...conditions for each cell line with a reporter construct to ensure robust plasmid delivery before attempting... that received high levels of the CRISPR/Cas9 constructs. Plate sorted cells individually into 96-well...

...experiments. You may have to design several different constructs to find one that could be used for the purpose...monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular ...

...including mutant, knockdown, and overexpression constructs. Each collection page listed below organizes ...Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene expression vectors for in...

...vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre Marillonnet and Johannes Stuttmann...containing several cassettes and generate multi-construct transgenic plants - Jan Lohmann. MoClo Plant Parts... for hierarchical assembly of plant multigene constructs - Johannes Stuttmann. MoChlo: Modular Cloning...

...Highlights Empty Backbones Expression Constructs Reporter Constructs Luciferase is the enzyme responsible...addition, Addgene has many luciferase expression constructs available that are driven by a strong constitutive...transcription Pete Stecha Return to Top Expression Constructs Luciferase driven by a strong constitutive promoter...in plants Daniel Voytas Return to Top Reporter Constructs Already know what gene or regulatory element ...reporter for? Browse or use the search box to find a construct that contains your regulatory element of interest...

... our inventory of chemogenetic constructs, including DREADD constructs....