We narrowed to 210 results for: cycs

1. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ...copies of the target DNA. Conventional thermal cycling is then performed to amplify the target using PCR...

2. A Quick Guide to a Career in Software Product Management

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   Blog Post

   ... the users, gather more feedback, and start the cycle over again to refine the solution, or to come up...

3. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   ...Cas9 expression is activated using 4-OH-T and doxycycline. A gRNA that targets your desired gene (whose...

4. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

   ... their study plans at a point in the research lifecycle when suggestions can be incorporated into the ...

5. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...OptoNBs. OptoNB coated beads were then imaged while cycling between dark and blue light which revealed a blue...

6. 7 Tips to Secure a STEAM Internship This Summer

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   Blog Post

   .... In order to gain a competitive advantage this cycle, consider the advice below:  1. Eligibility check...

7. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...toward this goal for years as members of the ENCyclopedia of DNA Elements Project (ENCODE), an NIH-funded...

8. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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   Blog Post

   ...Productize, (B) Roll out to market, and (C) Break even cycles. Work hard. Work fast. Be flexible. Be quick at...

9. All in a Twist: dsRNA

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   Blog Post

   ...acting on specific substrates and in distinct cell cycles. When RNA:DNA hybrids levels are allowed to reach...

10. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    ...CAV 1. Usually, caveolin 1 promotes the cell life cycle progression and enhances tumor growth, so it would...

11. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...1988) Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of...

12. RaPID Detection of RNA-protein Interactions

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    Blog Post

    ...untranslated regions (UTRs) to aid in the viral life cycle. Ramanathan et al. used RaPID to see what host proteins...

13. Choosing the B(right)est Fluorescent Protein: Photostability

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    Blog Post

    ...light reduces the number of excitation/emission cycles per unit of time, decreasing the likelihood that...

14. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post

    ...organelles to the vacuole/lysosome for degradation and recycling. The key to Rosella’s autophagy-sensing abilities...

15. CRISPRainbow and Genome Visualization

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    Blog Post

    ... to track telomere dynamics throughout the cell cycle. To optimize the signal to noise ratio of the system...

16. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    Blog Post

    ...upon activation. p-mcherry-C1-iC++_CA - A slow cycling (open for minutes) step-function artificial anion...

17. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    ...several times — some researchers report up to ten cycles! Just remember that a bit of sample is lost in ...

18. Viral Vectors 101: Virus Safety

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    ...meaning it can’t replicate and continue the infection cycle once inside the host. While the virus can still ...

19. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post

    ...You can clone your gene of interest into this tetracycline inducible, 3rd generation lentiviral vector ...

20. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ... are scraped, and lysed by multiple freeze-thaw cycles. The resultant lysate is your primary low titer...