...neighboring sequence context. Sci Signal 13. https://doi.org/10.1126/scisignal.aax9730  Additional resources on...90746-4 Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell...

...is continuously detected via a fluorescent signal. The signal increases proportionally to the amount of...concentration of the starting template DNA the fluorescent signal throughout the reaction is compared to a standard...

...identification of signaling pathways with potential to confer anticancer drug resistance." Science signaling 7.357...Carlsbad, CA) that represents 180 genes from the Ras signaling pathway. Its major appeal is that it enables the...

...decreasing the fluorescent signal from the first FP and increasing the signal from the second FP. Several...Addgene has many of these constructs and they are designated mGFP, mVenus, etc. A word of caution is needed...

...proteins, promoters, enhancers, polyadenylation signals, inducible gene-expression sequences, and more....promoter (to drive high-level expression) and a polyA signal (to terminate transcription) and the four constructs... containing multiple identical polyadenylation signals or promoters Gibson et al., 2009 MXS-Chaining...

...immunodetection tools is their strong signal strength. This signal strength is due in large part to their... be labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody (...

...fluorophore-linked antibodies are used to visualize RNA. Low signal-to-noise ratios with such techniques can be problematic...fluorophore and subsequently leading to a positive signal. The same effect can be achieved for a fluorophore...than separate them (Fig. 1b). One can also make “signal-off” biosensors in which the aptamers fluoresce...

...found that adding a triple nuclear localization signal or including fusions with Histone 2A or 2B can ...biotinylation via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by the desired nanobody...NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be exported to the periplasm...

...new improvements. The new variant has increased signal-to-noise ratio and better ability to record subthreshold...they are negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that... SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023 under a...

...Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those regulated by one-eyed pinhead—contribute... study also showed that in the absence of the signaling pathway regulated by one-eyed pinhead, incorrect...transform zygotes into adults and explain how signaling pathways control this process. Additional zebrafish...

... Fluorescent Protein Fusions Observing a bright signal for the first time after cloning an FP fusion with...illustrated the quantitative increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed... opposed to standard FPs. The increase in signal is not due to a brighter FP, but rather because of the...

...components can be electroporated into the oviduct of pregnant females, a technique called Genome editing via...injection into either an oocyte or into the oviduct of pregnant females (Yoon et al., 2018, Mizuno et al., 2018... it gets exciting – the cell sends out an “SOS” signal and cellular repair mechanisms rush in to repair...

...provides a high contrast image in which a bright signal overlays a perfectly black background. In addition...to more rapid photobleaching and a reduction in signal intensity. 2-Photon microscopes restrict excitation...light-reducing pinhole. By collecting more photons, a higher signal to noise ratio can be achieved (6). Super-resolution...

...the associated fluorochrome, emits a fluorescent signal that is captured by detectors. The strength of ... bind to a primary antibody thus amplifying the signal.       Intracellular protein targets, measured... FITC and that from PE. During compensation the signal arising from a specific fluorochrome is removed...

...you‘ll need, including sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014...protein degradation on chimeric antigen receptor signaling (Kim et al., 2024). The EMMA Toolkit is another...et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor Laboratory,...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...

...plasmid. The arrangement of the Nuclear Localization Signal (NLS) relative to the hCas9 coding region was optimized...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...