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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 921 results for: ire;

Showing: 101 - 120 of 921 results

  1. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...Simply cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP plasmid and 954 ng CMV-gvpNJKFGWV plasmid into...booster cassette), their expression heterogeneity required single cell screening to isolate a clonal cell...producing ultrasound contrast, and gene silencing required addition of chromatic-opening agents to boost ...contrast. It turned out that Anabaena mARGs did not require a booster cassette, either, allowing us to reduce...marked with Emerald GFP. These plasmids can be used directly in transient transfection in rtTA+ cell lines ...

  2. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of...

  4. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...CRISPR Activate and Interfere technologies also indirectly modify local chromatin state. Want more information...

  5. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... is an A->G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it...

  9. Plasmid Collections

    Type
    Collection
    ... are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...

  10. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...wiki entries describing enzyme activity, PAM requirements, experimental considerations, and more, created...

  13. AAV Packaged on Request

    Type
    Collection
    ...needs of many researchers, scientists sometimes require preps beyond our current in-stock capabilities....

  14. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...emission of a photon, the acceptor molecule is not required to be fluorescent. A variety of organic dyes ...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein abundance, size, and ...

  16. Open Enzyme Collection

    Type
    Collection
    ... new functions through genetic engineering and directed evolution. More information about the collection...

  18. Lentivirus Plasmids

    Type
    Collection
    ...lentivirus: these viruses are based on HIV-1, which may require additional lab biosafety procedures. Additionally...

  19. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... fluorescent protein downstream, separated by an IRES element. You did a lot of work! But let’s take a...-thi-1 or thi1 Mutation in thiamine metabolism Requires exogenous thiamine for growth ara disruption...leuB β-isopropyl malate dehydrogenase inactivated Requires exogenous leucine source for growth proAB mutation...mutation in proline biosynthesis pathway Requires exogenous proline source for growth rpsL Mutation ...This strain is endA+, so column-based DNA preps require an additional wash step. F- glnV44 recA13 mcrB ...
Showing: 101 - 120 of 921 results