We narrowed to 158 results for: itch

1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   ...https://doi.org/10.1038/nbt.2842 Wen Y, Liao G, Pritchard T, Zhao T-T, Connelly JP, Pruett-Miller SM, Blanc...

2. GPCRs: How Do They Work and How Do We Study Them?

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   Blog Post

   ...Heterotrimeric G proteins are important molecular switches in signal transduction pathways and consist of...

3. A Deep Dive into BioBuilder

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   Blog Post

   ...exhibit projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub teams’ ...

4. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...their favorite selection cassette. They could also switch from FLAG to GFP or a different epitope. Addgene...

5. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   Blog Post

   ...do a little bit of work for one client and then switch to doing something else. You’re never really integrated...

6. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

   Type

   Blog Post

   ...lets you fire neurons with the flick of a light switch. Neurons are not typically persuaded to fire when...

7. Choosing the B(right)est Fluorescent Protein: Photostability

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   Blog Post

   ...Shaner et al, 2008; Bindels et al, 2017) and photoswitching (Kremers et al, 2009), which usually are undesired...

8. CRISPRainbow and Genome Visualization

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   Blog Post

   ...but a newly expressed standard length gRNA would “switch” the system and induce genome editing. Such a system...

9. Control AAV Preps

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   Collection

   ...Fluorophore/Tag Green Red None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity...

10. Ras Pathway

    Type

    Collection

    ...binding protein) family. GTPases serve as molecular switches that transmit signals within the cell. GTPases...

11. Making CRISPR Plasmids Using Fragmid

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    Blog Post

    ...Because of its modular cloning approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters...

12. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ...methods lack spatiotemporal control or an “on/off switch.” This makes them great tools for providing a global...

13. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post

    ...J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...

14. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    ...-inducible degrons (AID). Further, the Yamamoto PITCh system plasmids provides an alternative to HDR as...

15. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    Blog Post

    ...store for us. If you’re thinking about making the switch from academia to entrepreneurship, share your thoughts...

16. Academic vs. Industry Postdocs

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    Blog Post

    ...after doing a brief academic postdoc. Why did I switch? Well, my long-term goal was to enter industry ...

17. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...potential platform that could be realized via simple switching of the relevant gRNAs and donor DNA Adenoviral...

18. Degrading DNA with Cascade-Cas3

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    Blog Post

    ...The Other Cas(s) CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems INTEGRATE: Bacterial Genome...

19. The Scientific Conference Poster Session: Tips for Success

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    Blog Post

    ...poster. Ask the presenter to give the “elevator pitch,” of his/her project – kindly let the presenter ...

20. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post

    ...through Non-Homologous End Joining Learn How the FLEx Switch Can Be Used to Turn Genes On and Off Resources...